Divalent egg yolk antibodies of type I and novel duck hepatitis as well as preparation method and application thereof

A technology of egg yolk antibody and duck hepatitis virus, which is applied in the preparation of new type duck hepatitis bivalent egg yolk antibody, egg yolk antibody preparation, type I field, can solve the problems of improper extraction method, extraction drug residue, lower potency, etc., and achieve therapeutic effect Obvious effects, high antibody purity, and small side effects

Active Publication Date: 2014-04-09
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at various technical defects existing in the prior art, one of the technical problems to be solved by the present invention is to provide a method for extracting egg yolk antibody, so as to solve the existing problem of improper extraction method and lack of antibody purification process, which leads to the extraction process In the process, a large amount of antibody is lost, the titer is greatly reduced, a large amount of miscellaneous proteins and fats are not removed, and there is a problem of residues of extracted drugs

Method used

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  • Divalent egg yolk antibodies of type I and novel duck hepatitis as well as preparation method and application thereof
  • Divalent egg yolk antibodies of type I and novel duck hepatitis as well as preparation method and application thereof
  • Divalent egg yolk antibodies of type I and novel duck hepatitis as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of Type I and New Type Duck Hepatitis Bivalent Egg Yolk Antibody

[0051] 1. Virus strains for production

[0052] The serum type Ⅰ duck hepatitis virus SD strain and the new type duck hepatitis virus JS strain used for vaccine preparation were isolated, identified, kept and supplied by Harbin Pharmaceutical Group Biological Vaccine Co., Ltd.

[0053] Serum Type Ⅰ Duck Hepatitis Virus SD Strain (DHAV-SD) is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and the microbial preservation number is: CGMCC No.8560.

[0054] The new duck hepatitis virus JS strain (DHAV-JS) is preserved in the common microorganisms of the China Microbiological Culture Collection Management Committee, and the microorganism preservation number is: CGMCC No.6852.

[0055] 2. Preparation of vaccine (immunogen)

[0056] 2.1 Preparation of stock solution of serum type Ⅰ duck hepatitis virus SD strain

[0057] Take serum t...

Embodiment 2

[0122] Example 2 Prevention and treatment effect of type Ⅰ and new duck hepatitis bivalent yolk antibody on type Ⅰ and new duck hepatitis

[0123] 1. Neutralization test

[0124] According to the current "Chinese Veterinary Pharmacopoeia" 2010 edition appendix neutralization test method fixed virus diluted serum method, after neutralizing the type I and new type duck hepatitis bivalent egg yolk antibody (prepared in Example 1) with the seed virus for inspection and serial dilution, respectively, The allantoic cavity was inoculated with 11-day-old duck embryos, observed continuously for 7 days, and measured. Results: The neutralizing titers of type Ⅰ and new duck hepatitis virus antibodies were both ≥1:512.

[0125] 2. Antivirus protection test

[0126] 200 3-day-old susceptible ducklings were selected for each of the 3 batches of antibodies, and randomly divided into groups 1 and 2, with 100 ducklings in each group. At the same time, 40 ducklings in the same batch were selec...

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Abstract

The invention discloses divalent egg yolk antibodies of type I and novel duck hepatitis as well as a preparation method and an application thereof. The preparation method comprises the steps of high-immunity egg collection, high-immunity egg sterilization, fat removal with sodium alginate, precipitation with ammonium sulfate, ultrafiltration membrane concentration and purification of the antibodies, bacteria removal by inactivation and the like. The research results show that the divalent egg yolk antibodies of type I and novel duck hepatitis, which are obtained through grease removal by adopting sodium alginate and precipitation by adopting ammonium sulfate, have high purity, high titers, small side effects and obvious treatment effects and can be used for simultaneously controlling type I and novel duck hepatitis viruses safely and conveniently.

Description

technical field [0001] The invention relates to a preparation method of egg yolk antibody, in particular to a preparation method of type I and new type duck hepatitis bivalent egg yolk antibody and its product and application. The invention belongs to the technical field of biological products for waterfowl. Background technique [0002] Duck hepatitis A (Duck Hepatitis A, DHA) is caused by duck hepatitis A virus (Duck Hepatitis A Virus, DHAV), an acute, highly fatal, contagious infectious disease of ducklings with liver as the main pathological change. The disease mainly affects ducklings aged 1 to 3 weeks. It spreads rapidly and the onset is severe. The main clinical symptoms are convulsions, convulsions and opisthotonos, with liver swelling and hemorrhage as the main pathological changes. Most of the sick ducks died within 1 hour after the onset of symptoms, and the mortality rate of 10-day-old ducklings after infection could reach more than 90%. This disease has become ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02C07K1/36C07K1/34C07K1/30C07K16/10A61K39/42A61P31/14
Inventor 宋扬王彬柴华藏玉婷于洪涛闫冰王昊陈海娟田野戴金玲
Owner 哈药集团生物疫苗有限公司
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