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Sterile collagen dressing and preparation method thereof

A collagen and sticking technology, applied in the field of sterile collagen dressing and its preparation, can solve problems such as application limitation of liquid collagen products, and achieve the effects of shortening purification time, increasing contact area and maintaining biological activity

Active Publication Date: 2015-06-24
GUANGZHOU TRAUER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid-enzyme mixing method is currently a commonly used extraction method in production. The efficacy and safety of collagen products have been further proved in clinical applications. At present, enterprises are able to produce collagen-related products on a large scale, but most of the products can only reach the microbial limit level. Even if a few can reach the sterile level, they can only be provided in the form of dry products for clinical applications, resulting in The clinical application of liquid collagen products is limited

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  • Sterile collagen dressing and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Degrease and remove impurities from fresh animal tendon tissues, then clean, slice and disinfect;

[0030] 2) Use a pulverizer to crush the disinfected tendon tissue;

[0031] 3) Dissolve the crushed tendon tissue with acid enzyme compound method for 12 to 72 hours, that is, add 100 times the volume of 0.5mol / L glacial acetic acid and 400mg / L pepsin to the tendon tissue to dissolve 12 ~72h, the collagen solution with triple helix structure can be extracted;

[0032] 4) Centrifuge the extracted collagen solution to remove the precipitate, and collect the supernatant to obtain the original collagen solution;

[0033] 5) Adjust the concentration and viscosity of the original collagen solution, the concentration is adjusted to 2.5mg / ml (the concentration range can be set to 0.5~3.0mg / ml), after purification and concentration by ultrafiltration, high purity and high concentration collagen solution can be obtained ;

[0034] 6) The collagen solution is vacuum freeze-dried and then...

Embodiment 2

[0040] 1) Degrease and remove impurities from fresh animal tendon tissues, then clean, slice and disinfect;

[0041] 2) Use a pulverizer to crush the disinfected tendon tissue;

[0042] 3) The pulverized tendon tissue is dissolved for 50h by the acid enzyme compound method, that is, 80 times the volume of 0.6mol / L glacial acetic acid and 420mg / L pepsin are added to the tendon tissue to dissolve for 50h, namely Can extract collagen solution with triple helix structure;

[0043] 4) Centrifuge the extracted collagen solution to remove the precipitate, and collect the supernatant to obtain the original collagen solution;

[0044] 5) Adjust the concentration and viscosity of the original collagen solution, the concentration is adjusted to 2.5mg / ml (the concentration range can be set to 0.5~3.0mg / ml), after purification and concentration by ultrafiltration, high purity and high concentration collagen solution can be obtained ;

[0045] 6) The collagen solution is vacuum freeze-dried and the...

Embodiment 3

[0051] 1) Degrease and remove impurities from fresh animal tendon tissues, then clean, slice and disinfect;

[0052] 2) Use a pulverizer to crush the disinfected tendon tissue;

[0053] 3) Dissolve the crushed tendon tissue with acid enzyme compound method for 12 hours, that is, add 120 times the volume of 0.4 mol / L glacial acetic acid and 390 mg / L pepsin to the tendon tissue to dissolve for 12 hours, namely Can extract collagen solution with triple helix structure;

[0054] 4) Centrifuge the extracted collagen solution to remove the precipitate, and collect the supernatant to obtain the original collagen solution;

[0055] 5) Adjust the concentration and viscosity of the original collagen solution, the concentration is adjusted to 2.5mg / ml (the concentration range can be set to 0.5~3.0mg / ml), after purification and concentration by ultrafiltration, high purity and high concentration collagen solution can be obtained ;

[0056] 6) The collagen solution is vacuum freeze-dried and then ...

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Abstract

The invention discloses a sterile collagen dressing and a preparation method thereof. The preparation method of the sterile collagen dressing comprises the following steps: degreasing an animal tendon, slicing, disinfecting, smashing, performing acid soluble enzymolysis, centrifuging and removing the precipitate to obtain a collagen stock solution; adjusting the concentration and the viscosity of the collagen stock solution, performing ultrafiltration and purification, and condensing to obtain the collagen solution with high purity and high concentration; performing freeze drying, smashing and performing irradiation sterilization, dosing and filling into an aluminum foil bag containing a non-woven. The activity of the collagen is kept, and the degeneration of the collagen due to the irradiation or the cross-linking reaction can be avoided; the traditional dialysis purification is replaced by the ultrafiltration and purification so that the purifying time is shortened; the traditional semiautomatic operation is replaced by an automatic system, so that the hand pollution is avoided, and the cleanliness, purity and biological activity of the product are guaranteed.

Description

Technical field [0001] The present invention involves a sterile gum -ray dressing and preparation method. Background technique [0002] Collagen is the most important water insoluble fibrin in extracellular, and is a skeleton that constitutes extracellular matrix.The collagen forms a semi -crystal fiber in the extracellular matrix, providing cells with anti -tension and elasticity, and plays a role in cell migration and development.Collagen exists in various animals.The collagen in the tendon, cartilage and bone in the vertebrate is very rich, almost half of the total protein weight.The collagen is twisted into spiral -shaped fiber -shaped extracellular proteins from the three peptide chains, accounting for more than 30% of the whole body protein, and has unique physiological activity.Collagen is rich in amino acids such as glycine, proline, hydroxyticine for the human body. It is the most important part of the extracellular matrix. It has strong biological activity and biologica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/42A61K8/65A61Q19/00
Inventor 李奕恒陈淡嫦
Owner GUANGZHOU TRAUER BIOTECH
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