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Heme protein functional magnetic surface enhanced Raman active substrate and application thereof

A surface-enhanced Raman, heme protein technology, applied in Raman scattering, preparation of test samples, material excitation analysis, etc., can solve problems such as loss and protein function defects

Inactive Publication Date: 2015-01-07
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The toxicity mechanism of some small molecules is that these small molecules can bind to the heme protein in the organism, thus causing the defect or even loss of the function of these proteins

Method used

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  • Heme protein functional magnetic surface enhanced Raman active substrate and application thereof
  • Heme protein functional magnetic surface enhanced Raman active substrate and application thereof
  • Heme protein functional magnetic surface enhanced Raman active substrate and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Heme protein functionalized magnetic surface-enhanced Raman active substrate applied to cyanide detection

[0031] 1) Stir vigorously in a nitrogen atmosphere containing 0.001M FeCl 2 4H 2 O and 0.002M FeCl 3 ·6H 2 O 100mL mixed solution for 30min, then add 15mL concentrated ammonia water (25%, w / w) dropwise, continue stirring for 30min, heat at 80°C for 30min, wash with ethanol and distilled water for 3 times respectively, and obtain Fe 3 o 4 Nanoparticles.

[0032] 2) In order to improve its dispersibility, disperse 100mg of magnetic nanoparticles obtained in the above steps into 200mL of sodium citrate solution with a concentration of 0.3M, stir mechanically at 80°C for 1 hour, then separate with a magnet, and then use ethanol and Rinse with pure water 3 times and dry naturally.

[0033] 3) 50mg Fe after the above-mentioned sodium citrate pretreatment 3 o 4 Nanoparticles were dispersed in 120mL ethanol, 40mLH 2 O and 3mL ammonia water mixed soluti...

Embodiment 2

[0040] Example 2: Heme protein functionalized magnetic surface-enhanced Raman active substrate applied to nitrite detection

[0041] Same as the steps in Example 1, the difference is:

[0042] In step 7), the magnetic silver nanoparticles covalently adsorbed by myoglobin are dispersed into sodium nitrite solutions of different concentrations (1ppb, 100ppb, 10ppm).

[0043] Step 8) Add 20 μL of sodium dithionite (10 mg / mL) to each sample during the measurement. In this step, sodium dithionite can reduce the oxidized myoglobin to the reduced form, and only the reduced form of myoglobin absorbs nitrous acid Capable of producing 1375cm after salt -1 Raman characteristic peak at (other examples do not need to add sodium dithionite).

Embodiment 3

[0044] Example 3: The dual-component detection of cyanide and hydrogen peroxide applied to the heme protein functionalized magnetic surface-enhanced Raman active substrate

[0045] As in the steps of Example 1, the difference is:

[0046] In step 7), the magnetic silver nanoparticles covalently adsorbed by myoglobin were dispersed into a mixed solution of 10 μM cyanide and 10 μM hydrogen peroxide (volume ratio 1:1).

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Abstract

The invention relates to a heme protein functional magnetic surface enhanced Raman active substrate and an application thereof to the high-sensitivity detection of toxic micro-molecules and the rapid removal of the toxicity of the toxic micro-molecules, and belongs to the technical field of monitoring and detecting toxic ingredients in environment and food. The heme protein functional magnetic surface enhanced Raman active substrate is prepared by the following steps: synthesis, preparation and coating of a silver nano magnetic material, covalent adsorption of heme protein and spectrum detection of surface enhanced Raman scattering (SERS) and the like. The heme protein functional magnetic surface enhanced Raman active substrate and the application thereof have the beneficial effects that through the absorption of some toxic micro-molecules, the displacement of a heme protein resonance Raman peak can be caused, and the displacement has the molecular specificity, therefore, the micro-molecules can be subjected to multi-component detection by the utilization of an SERS spectrum; with the addition of the aggregation of magnetic nano materials under the magnetic field conditions, the concentration of molecules to be detected and the SERS enhancement capacity can be improved. The silver nano magnetic material in the heme protein functional magnetic surface enhanced Raman active substrate has a great application potential in the fields of food safety, environment monitoring and protection and the like.

Description

technical field [0001] The invention belongs to the technical field of monitoring and detection of toxic components in the environment and food, and in particular relates to a heme protein functionalized magnetic surface-enhanced Raman active substrate and its high-sensitivity detection of toxic small molecules and its rapid removal of toxicity application. Background technique [0002] Environmental and food safety issues are closely related to people's daily life. The toxicity of some small molecules (cyanide, nitrite, nitric oxide, carbon monoxide, etc.) commonly found in the environment and food has potential threats to people's health. Cyanide is widely found in food and plants in nature, as well as in car exhaust and cigarette smoke in the environment. Cyanide ions have a strong complexing ability for heavy metal ions, and can inhibit the activity of metalloenzymes, thereby inhibiting the electron transport chain in cellular respiration. Nitrite is often used as a co...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/34
Inventor 韩晓霞陈雷王旭赵冰
Owner JILIN UNIV
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