Electrical chemiluminescence immunoassay based on gold magnetic particles

A technology of luminescent immunity and gold magnetic particles, which is applied in the field of medical testing, can solve the problems of lack of versatility, poor controllability of the reaction, and cumbersome steps, and achieve the effects of low labeling cost, easy operation, and simplified experimental steps
CN102721804AInactive Publication Date: 2012-10-10XIAN GOLDMAG NANOBIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIAN GOLDMAG NANOBIOTECH
Publication Date
2012-10-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides an electrical chemiluminescence immunoassay method based on gold magnetic particles, which realizes the quantitative detection on the content of antigen / antibody in human serum, blood plasma, cell culture supernatant or other related biological liquid so as to solve the problems of high cost, complicated steps, poor reaction controllability, no generality and the like in the prior art. The method mainly comprises the steps of 1) preparing immunomagnetic beads by gold magnetic particles; 2) labeling the antigen / antibody by Ru(bpy) <2+>3; 3) establishing a standard curve for quantitatively detecting the antigen / antibody; and 4) detecting a sample to be detected. A novel electrical chemiluminescence immunoassay technology is adopted, and the electrical chemiluminescence immunoassay method based on gold magnetic particles has the advantages that the operation is simple and convenient, the valence of antibody can not be affected in the process that the antibody is labeled by Ru(bpy) <2+>3, the labeling cost is low, and the universality is realized.
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Description

technical field

[0001] The invention belongs to the field of medical inspection, and in particular relates to electrochemiluminescence immunoassay technology. Background technique

[0002] The concentration of a certain antigen / antibody in the human body may be directly related to its health status, physiological function, and physiological cycle, so quantitative detection of the concentration of antigen / antibody in the human body is of great significance to the human body. For example, the concentration of PSA in human serum is of great significance in the diagnosis, prognosis and curative effect detection of prostate cancer. At present, the detection techniques for antigens / antibodies in human serum, plasma, cell culture supernatant or other related biological fluids mainly include: Radioimmunoassay (RIA), Enzyme linked Immunosorbent Assay (ELISA), Fluorescence Immunoassay (FIA), Chemiluminescence Immunoassay (CLIA), Immunochromatography (Lateral Flow Immunoassay, LFIA) a...

Claims

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