Method for preparing adipose derived stem cell bioactive peptide freeze-dried powder

Abstract

The invention provides a method for preparing adipose derived stem cell bioactive peptide freeze-dried powder. The preparation method includes the following steps that S1, autologous fat tissue is extracted; S2, primary-generation fat stem cells are prepared from the autologous fat tissue; S3, fifth-generation fat stem cells are prepared from the primary-generation fat stem cells, and cell culture supernatant fluid is obtained; S4, the cell culture supernatant fluid is centrifuged, a supernatant solution is collected, and the supernatant solution is filtered; S5, after ultrafiltration and concentration are carried out, a freeze-drying protective additive is added for freeze drying, and the adipose derived stem cell bioactive peptide freeze-dried powder is obtained. Through a series of operating steps, an active factor mixture in adipose derived stem cell culture supernatant is preserved in the form of freeze-dried powder, obtained factors are completely derived from autografts, use is safe and reliable, and the possibility of allergy and infection of exogenous pathogens in the using process is eliminated.

Description

technical field [0001] The present invention relates to a preparation method of autologous adipose stem cell bioactive peptide freeze-dried powder, which belongs to the field of biotechnology, in particular to a preparation method of autologous bioactive peptide, which can effectively prepare high-quality autologous stem cell-derived biological active peptide. Background technique [0002] In recent years, with the development of science and technology and the improvement of living standards, people's demand for beauty and anti-aging has exploded. The beauty and anti-aging means and methods that were born thereupon also came into being. Including the once famous sheep placenta, human placenta and stem cell anti-aging and so on. Although sheep placenta and placenta have a good user experience, they are derived from heterogeneous and heterogeneous sources, which involve ethical issues and risk control issues. They need to be used with caution and are also very expensive. ...

AI Technical Summary

Problems solved by technology

In addition, although the stem cell bioactive peptides and stem cell seeds from allogeneic sources have been screened, there is still a risk of infection

There are also some products that are directly obtained by directly lysing stem cells or other active cells. The protein composition is complex. Although the concentration is high, there are many ineffective proteins, which will also cause metabolic stress on the human body.

For example, the patent with the notification number CN106381284A uses allogeneic umbilical cord-derived stem cells to collect proteins and polypeptides by ultrasonic crushing. A large number of proteins obtained by this method are intracellular proteins. Although the concentration is high, most of them do not have the ability to regulate or regulate skin cells The function of the repaired factors, and the umbilical cord stem cells obtained by large-scale allogeneic still have the risk of infection

[0007] In addition, in addition to the risk of infection caused by the use of allogeneic stem cells, the use of a large amount of heterogeneous serum is also an important risk factor. Allergies of the applicable population to fetal bovine protein will directly lead to a decrease in user experience. For example, the patent with publication number CN102465148 A uses fetal bovine serum As the main component of the medium, fetal bovine serum has complex components and will directly enter the product, causing allergy risks

Images