Synthesizing method of L-carnosine

Abstract

The invention discloses a production method of carnosine, which is characterized by comprising the following steps of: synthesizing N-acetyl cyanide-L-histidine through ammonolysis reaction by using L-histidine and ethyl cyanoacetate as raw materials and sodium alcoholate as a catalyst; and then obtaining L-carnosine through the intermediate by using stronger ammonia water and alcohol as a solvent in a high-pressure reaction kettle and using a Pd-Rh/C composite catalyst for catalytic hydrogenation under the protection of nitrogen, wherein the overall yield of two-step reaction achieves 72 percent. The invention has simple process, easy operation, low cost, and the like and is very suitable for industrialized production.

Description

technical field The invention relates to the fields of food additives and medicines, in particular to a production method of L-carnosine. technical background Carnosine is a natural dipeptide present in animal muscle, which can inhibit the fat oxidation reaction caused by iron, copper, myoglobin and lipoxygenase. Since it comes from animal muscle, its antioxidant effect is very important in food preservation has wide application. In addition, carnosine also shows various physiological effects, such as anti-neurodegeneration, anti-epileptic effect, anti-narcotic drug addiction effect, anti-protein cross-linking effect, anti-arteriosclerosis and anti-ulcer effect. Develop application value. The existing method for preparing carnosine mainly contains following several kinds: 1. Extraction from animal muscle Carnosine is directly extracted from carnosine meat of mammals such as beef and pork, but the production of carnosine is limited due to the low content in animal muscle...

AI Technical Summary

Problems solved by technology

But this method is easy to generate peptides, the product quality is not easy to control, and toxic sulfides are easy to cause serious environmental pollution

Skoblik et al. and Zhang Guolin et al. used Boc-β-alanine or N-benzyloxycarbonyl-β-alanine as raw materials to react with succinyl hydroxylimide respectively to obtain the corresponding N-hydroxysuccinyl hydroxylimide ester , and then react with L-histidine to obtain a dipeptide, and remove the protective group to obtain L-carnosine. This method has cumbersome steps and high production costs, and is also difficult to be suitable for industrial production.