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Injection for correcting skin wrinkles and preparation method thereof

An injection and fold technology, applied in the fields of implantable medical devices and skin nutrition, can solve the problem of slow increase of antioxidant effect, and achieve the effect of solving skin folds and relaxation, low viscosity and promoting synthesis.

Active Publication Date: 2014-12-10
IMEIK TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that L-carnosine has a good antioxidant effect at about 10mmol / L, and then with the concentration increasing, the antioxidant effect increases slowly

Method used

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  • Injection for correcting skin wrinkles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Intradermal injection formula: sodium hyaluronate (4g / L), L-carnosine (2g / L), glycamino acid (50mg / L), alanine (50mg / L), proline (100mg / L) , Vitamin B 2 (1 mg / L).

[0038] Prepare 1 L of sample according to the above formula: Dissolve sodium hyaluronate, L-carnosine and amino acids in 900 mL of water for injection to obtain solution A; prepare water-soluble vitamins (or their analogs) into 0.5% concentrated solution (mass fraction) Then add it to solution A to obtain solution B; adjust the pH value of solution B to 7.2±0.2 with 2M hydrochloric acid, and adjust the osmotic pressure to 70±10mOsm / L with sodium chloride. Then, transfer solution B to a 1L volumetric flask, and dilute to 1L with water for injection. The obtained B solution was filtered and sterilized through a 0.2 μm needle filter membrane (manufacturer: PALL) in a sterile environment, and a sterile transparent liquid sample was obtained.

[0039] Safety of the sample: The cytotoxicity test is carried out ...

Embodiment 2

[0042] The sample prepared in Example 1 was diluted 4 times with DMEM medium (manufacturer: Gibco) containing 10% neonatal bovine serum (manufacturer: hyclone) and used to culture mouse fibroblast L929 as the experimental group; normal saline It was also used to culture L929 cells after diluting 4 times with DMEM medium as a control group. After 48 hours, the supernatant culture medium was taken, and the content of collagen was measured by the Woessner method. The results are shown in Table 1. It can be seen that the amount of collagen synthesis in the experimental group increased by nearly 3 times compared with the control group.

[0043] Test items control group test group P value Collagen 100±8% 373±10% <0.001

Embodiment 3

[0045] Firstly, a mouse aging model was made, and the specific modeling method was as follows: 9-month-old BABL / c female mice were subcutaneously injected with D-galactose at the back of the neck, once a day, for 4 consecutive weeks. The initial dose is 70mg / (kg) for 7 days; followed by 140mg / (kg) for 7 days; the final injection is 180mg / (kg) for 14 days. After the modeling was completed, physiological saline and the sample prepared in Example 1 were respectively injected on the left and right sides of the spine of the D-galactose-induced aging mouse model. Samples or saline were injected every other week, and the sample volume injected at each site was 50 μL, with 3 points on each side. A total of three injections were performed, and the experimental animals were sacrificed one week after the last sample injection, and the skin tissue at the injection site was removed and the subcutaneous fat was discarded. Collagen content in the skin of the injection site was measured usin...

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Abstract

The invention relates to an injection (intracutaneous injection) for correcting skin wrinkles and a preparation method thereof. The injection is prepared from the following components by mass: 3-6g / L of high molecular polysaccharide, 50-200mg / L of amino acid, 2-10mg / L of water-soluble vitamin and 2-5g / L of L-carnosine. A sample prepared according to the formula is capable of supplying extracellular matrix to skin and promoting synthesis of spontaneous collagen of skin. The injection has the function of a traditional filling material for supplying lost extracellular matrix, is capable of promoting synthesis of collagen and solving the problem of wrinkled and loose skin caused be senility. Furthermore, compared with a conventional filling material, the injection is lower in viscosity and is more suitable for intracutaneous injection. According to the injection prepared by the method, the problem of sodium hyaluronate and collagen degradation of skin in the aging process can be solved by means of the intracutaneous injection, and an anti-wrinkle and skin-firming effect can be achieved.

Description

technical field [0001] The invention relates to an injection for correcting skin folds (intradermal injection) and a preparation method thereof. The formula of the product includes high molecular polysaccharides, amino acids, water-soluble vitamins and L-carnosine. The sample prepared according to this formula can replenish the extracellular matrix to the skin and promote the synthesis of the skin's own collagen. The invention relates to the fields of implantable medical devices, skin nutrition and the like. Background technique [0002] The skin of the human body will age due to the combined action of internal and external factors. As aging occurs, changes occur inside the skin, for example, reduction of extracellular matrix; degradation of collagen. These are the root causes of skin wrinkles and sagging. Therefore, to solve problems such as skin shrinkage and sagging, it is necessary to start from the root cause, such as supplementing the extracellular matrix and collag...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22
Inventor 简军陈雄伟张政朴李睿智
Owner IMEIK TECH DEV CO LTD
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