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Chicken spleen organ hydrolysate and preparation method and application thereof

A hydrolyzate and spleen technology, applied in the field of chicken spleen hydrolyzate, can solve the problems of long production cycle, low added value, single function, etc., and achieve the effect of shortened production cycle, high added value and simple production process

Inactive Publication Date: 2016-06-15
山东省健牧生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the problems existing in the prior art, the purpose of the present invention is to provide a chicken spleen hydrolyzate prepared by enzymatic method, which solves the problems of low added value, single function, long production cycle, cumbersome steps and high cost of the traditional method.

Method used

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  • Chicken spleen organ hydrolysate and preparation method and application thereof
  • Chicken spleen organ hydrolysate and preparation method and application thereof
  • Chicken spleen organ hydrolysate and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] A preparation method for chicken spleen hydrolyzate, comprising the following steps:

[0030] (1) Clean the chicken spleen with the outer fat, epidermis and subcutaneous fascia removed with purified water, add normal saline at a weight ratio of 1:1, and then add Gur gum and gelatin (ancient The mass ratio of gelatin and gelatin is 1:3), pour it into a colloid mill, shear and grind at 3900r / min for 30s, and obtain a homogenate;

[0031] (2) Take the homogenate into the reactor, add purified water at a volume ratio of 1:1 and mix evenly to obtain a mixed solution, add 4000U compound protease to each 16ml of the homogenate, adjust the pH value of the mixed solution to 6.2, and store it at 50°C Under hydrolysis for 3.5 hours, the hydrolyzate was obtained;

[0032] (3) Centrifuge the hydrolyzate at 3000r / min for 10 minutes, pass the supernatant through a 10KDa hollow fiber ultrafiltration column to remove the complex protease, and obtain an extract, add potassium sorbate ac...

Embodiment 2

[0034] A preparation method for chicken spleen hydrolyzate, comprising the following steps:

[0035] (1) Clean the chicken spleen with the outer fat, epidermis and subcutaneous fascia removed with purified water, add normal saline at a weight ratio of 1:1, and then add Gur gum and gelatin (ancient The mass ratio of gelatin and gelatin is 1:3), pour it into a colloid mill, shear and grind at 3900r / min for 30s, and obtain a homogenate;

[0036] (2) Take the homogenate into the reactor, add purified water at a ratio of 1:1 and mix evenly to obtain a mixed solution, add 3000U of papain to each 16ml of the homogenate, adjust the pH of the mixed solution to 6.2, and hydrolyze at 50°C for 4 hour, collect the hydrolyzate;

[0037] (3) Centrifuge the hydrolyzate at 4000r / min for 5 minutes, pass the supernatant through a 10KDa hollow fiber ultrafiltration column to remove papain, and obtain an extract, add potassium sorbate accounting for 0.01% by weight of the extract to obtain chicke...

Embodiment 3

[0039] A preparation method for chicken spleen hydrolyzate, comprising the following steps:

[0040] (1) Clean the chicken spleen with the outer fat, epidermis and subcutaneous fascia removed with purified water, add normal saline at a weight ratio of 1:1, and then add Gur gum and gelatin (ancient The mass ratio of gelatin and gelatin is 1:3), pour it into a colloid mill, shear and grind at 3900r / min for 30s, and obtain a homogenate;

[0041] (2) Take the homogenate into the reactor, add purified water at a ratio of 1:1 and mix evenly to obtain a mixed solution. According to the ratio of adding papain 4000U and compound protease 2000U to each 16ml homogenate, first add papain to adjust the mixed solution The pH value is 6.0, hydrolyze at 50°C for 1 hour, then add complex protease, adjust the pH value of the mixture to 6.6, continue hydrolysis for 2 hours, and collect the hydrolyzate;

[0042] (3) Centrifuge the hydrolyzate at 4000r / min for 7 minutes, pass the supernatant thro...

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Abstract

The invention relates to chicken spleen organ hydrolysate obtained through enzymatic hydrolysis and a preparation method and application thereof and belongs to the biotechnology field. The chicken spleen organ hydrolysate is prepared through the following steps that firstly, surface fat, epidermis and chicken organs of subcutaneous fascia are removed, cleaning is performed through purified water, physiological saline is added, gul glue and gelatin are added, shearing and grinding are performed, and homogenate is obtained; the homogenate is placed in a reactor, protease is added for hydrolysis, and hydrolysate is obtained; finally, the hydrolysate is centrifuged, protease is removed, an extracted solution is obtained, potassium sorbate is added, and the chicken spleen organ hydrolysate is obtained. Compared with traditional feed, the protein digestibility of the prepared product is remarkably improved, the prepared product is free of bitter taste and has the functions of improving cellular immunity and humoral immunity, additional value is high, the production period is short, and the chicken spleen organ hydrolysate is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a chicken spleen hydrolyzate obtained by enzymatic hydrolysis, its preparation method and application. technical background [0002] China is a big poultry farming country, with poultry meat output reaching 17.51 ​​million tons in 2014 (National Bureau of Statistics). The chicken spleen is the immune organ of the chicken, accounting for about 0.2% of the chicken's body weight, with an annual output of 35,000 tons. Chicken spleen is generally processed together with chicken intestines and pancreas as leftovers at a low price. The traditional preparation method is to heat and pressurize the leftovers of chicken spleen to make animal-derived feed, which is fed to animals and supplemented with crude protein and trace elements. However, the feed product obtained by the traditional method has single function and low added value, which is not conducive to the development and u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/26A23K10/14A23K20/147
Inventor 黄迪海秦卓明张再辉刘霞徐秀荣盛晓丹
Owner 山东省健牧生物药业有限公司
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