Method for extracting Chinese yam polypeptide

Abstract

The invention provides a method for extracting Chinese yam polypeptide, and belongs to the field of biotechnology, wherein the method particularly comprises the steps: 1) peeling fresh Chinese yam, crushing, grinding into pulp, adding water, heating to 45-55 DEG C, and carrying out ultrasonic extraction to obtain an extract liquid; 2) after the extract liquid in the step 1) is denaturated, heating, adding a compound enzyme A and a compound enzyme B, and carrying out enzymatic hydrolysis; 3) carrying out inactivated treatment on the enzymatic hydrolysate of the step 2), standing, and separatingand purifying to obtain a supernatant; and 4) carrying out molecular weight classification of the supernatant of the step 3), debitterizing, concentrating and drying to obtain the Chinese yam polypeptide. The method has the beneficial effects that the method adopts an interlaced interval treatment method of dual-frequency composite ultrasound and microwave radiation to promote the denaturation ofChinese yam proteins, and greatly improves the hydrolysis efficiency and the yield of the Chinese yam polypeptide; acetic acid can eliminate the bitterness of the Chinese yam polypeptide, improves palatability and edibility, and expand an application range; the Chinese yam polypeptide has great application potential in inhibiting insuloma cells, preventing and treating diabetes, relieving glucosetolerance and lowering blood sugar.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting yam polypeptide. Background technique

[0002] Yam, also known as yam, native potato, yam, Huai yam, Huaishan, and white yam, is a herbal medicine recorded in "Chinese Materia Medica", and its medicinal source is the dried rhizome of yam, a plant of the family Dioscoreae. As a traditional Chinese medicinal material for both medicine and food, yam has different origin characteristics due to the influence of regional climate characteristics, geological characteristics, growth habits and other factors. Yam is mainly produced in Boai, Wuzhi, Wenxian and other places in Henan, and it is also cultivated in Shanxi, Shaanxi, Shandong, Hebei, Zhejiang, Hunan, Sichuan, Yunnan, Guizhou, Guangxi and other places. Several major production areas mainly in Guangxi, Hebei, Henan and other places constitute the main yam cultivation areas in China.

[0003] Yam is...

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