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Method for extracting Chinese yam polypeptide

A yam polypeptide and extraction method technology are applied in the directions of pharmaceutical formulations, food ingredients containing natural extracts, drug combinations, etc., to achieve the effects of eliminating bitterness, improving the degree of hydrolysis, and being environmentally friendly.

Inactive Publication Date: 2019-09-06
海盐县凌特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no well-developed compounds extracted from yams for the prevention or treatment of diabetes

Method used

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  • Method for extracting Chinese yam polypeptide

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Experimental program
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Effect test

Embodiment 1

[0050] A method for extracting yam polypeptide, specifically comprising:

[0051] 1) Fresh yam is peeled, crushed, and refined into a slurry, adding 5 times the weight of water, heated to 45°C, and ultrasonically extracted for 15 minutes to obtain the extract; fresh yam is ground into a slurry and then extracted with ultrasonic waves, which can effectively separate the crude oil in the yam. Protein, crude fiber and starch greatly increase the degree of dispersion of the above substrates and prepare for further enzymatic hydrolysis;

[0052] 2) The extract in step 1) is denatured and heated to 65°C, and compound enzyme A and compound enzyme B are added for enzymatic hydrolysis; the yam slurry is enzymatically hydrolyzed with multiple compound enzymes, which can maximize the coordinated gain of various enzymes Its enzymatic hydrolysis activity, efficiency and speed far exceed that of a single active enzyme, which significantly shortens the enzymatic hydrolysis time, accelerates ...

Embodiment 2

[0078] This embodiment provides item [1]: a method for extracting yam polypeptide, which specifically includes:

[0079] 1) Fresh yam is peeled, crushed and refined into a slurry, adding 10 times the weight of water, heated to 52°C, and ultrasonically extracted for 25 minutes to obtain an extract; after fresh yam is refined into a slurry, it can be extracted by ultrasonic wave, which can effectively separate crude oil in yam. Protein, crude fiber and starch greatly increase the degree of dispersion of the above substrates and prepare for further enzymatic hydrolysis;

[0080] 2) The extract in step 1) is denatured and heated to 70°C, and compound enzyme A and compound enzyme B are added for enzymatic hydrolysis; the yam slurry is enzymatically hydrolyzed with multiple compound enzymes, which can maximize the coordinated gain of various enzymes Its enzymatic hydrolysis activity, efficiency and speed far exceed that of a single active enzyme, which significantly shortens the enz...

Embodiment 3

[0114] Example 3 is basically the same as Example 2, except that no denaturation operation is performed in Example 3.

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Abstract

The invention provides a method for extracting Chinese yam polypeptide, and belongs to the field of biotechnology, wherein the method particularly comprises the steps: 1) peeling fresh Chinese yam, crushing, grinding into pulp, adding water, heating to 45-55 DEG C, and carrying out ultrasonic extraction to obtain an extract liquid; 2) after the extract liquid in the step 1) is denaturated, heating, adding a compound enzyme A and a compound enzyme B, and carrying out enzymatic hydrolysis; 3) carrying out inactivated treatment on the enzymatic hydrolysate of the step 2), standing, and separatingand purifying to obtain a supernatant; and 4) carrying out molecular weight classification of the supernatant of the step 3), debitterizing, concentrating and drying to obtain the Chinese yam polypeptide. The method has the beneficial effects that the method adopts an interlaced interval treatment method of dual-frequency composite ultrasound and microwave radiation to promote the denaturation ofChinese yam proteins, and greatly improves the hydrolysis efficiency and the yield of the Chinese yam polypeptide; acetic acid can eliminate the bitterness of the Chinese yam polypeptide, improves palatability and edibility, and expand an application range; the Chinese yam polypeptide has great application potential in inhibiting insuloma cells, preventing and treating diabetes, relieving glucosetolerance and lowering blood sugar.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting yam polypeptide. Background technique [0002] Yam, also known as yam, native potato, yam, Huai yam, Huaishan, and white yam, is a herbal medicine recorded in "Chinese Materia Medica", and its medicinal source is the dried rhizome of yam, a plant of the family Dioscoreae. As a traditional Chinese medicinal material for both medicine and food, yam has different origin characteristics due to the influence of regional climate characteristics, geological characteristics, growth habits and other factors. Yam is mainly produced in Boai, Wuzhi, Wenxian and other places in Henan, and it is also cultivated in Shanxi, Shaanxi, Shandong, Hebei, Zhejiang, Hunan, Sichuan, Yunnan, Guizhou, Guangxi and other places. Several major production areas mainly in Guangxi, Hebei, Henan and other places constitute the main yam cultivation areas in China. [0003] Yam is...

Claims

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Application Information

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IPC IPC(8): A23L33/18A61K38/01A61P3/10C12P21/06C07K1/34
CPCA23L33/18A23V2002/00A61K38/011A61P3/10C07K1/34C12P21/06A23V2200/328A23V2250/21
Inventor 董珊珊
Owner 海盐县凌特生物科技有限公司
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