55results about How to "The synthesis procedure is simple" patented technology

The invention relates to a rapid preparation method of a transparent mesoporous silica gel monolith, which belongs to the technical field of the inorganic porous material, in particular to a technical scheme of a simple, efficient, rapid and convenient preparation method of a large-sized, crack-free and optically transparent ordered mesoporous silica gel monolith. The preparation method is characterized in that the direction conversion of gel melting, condensation, aging and drying can be realized at a high temperature through adjusting a synthesis ratio without special protection means and complex operating procedures. Moreover, the method has the advantages of short synthesis period, simple operating steps and easy control. The material prepared in the method has an ordered two-dimensional and hexagonal-phase mesoporous structure, the pore size is 2.0-7.0nm, the pore volume is 0.5-0.8 cm3 / g, and the specific surface area is 500-1000m2 / g. Therefore, the material has extensive application value in research and development of optical, electrical and magnetic devices.

The invention discloses a double-mesopore silicon dioxide transparent gel monolith and a preparation method thereof, which belong to the technical field of inorganic porous materials. The invention particularly relates to a large-size, flawless and transparent silicon dioxide gel monolith with double-mesopore distribution characteristics and a fast preparation method thereof. The silicon dioxide gel monolith is characterized in that the double-mesopore pore-size distribution of the silicon dioxide gel monolith is in a mesoporous region, and is specifically characterized in that: the double-mesopore silicon dioxide transparent gel monolith is provided with the combination of a cylindrical primary mesopore and a worm-like secondary mesopore, and the N2 adsorption isotherms of the cylindrical primary mesopore and the worm-like secondary mesopore are type IV curves having characteristics of a type H1 hysteresis loop. The preparation method has a short synthetic cycle and simple operating steps and is easy to control, the prepared double-mesopore silicon dioxide transparent gel monolith containing a surfactant has a regular shape, a controllable size and high thermal stability and transparency, and is suitable for manufacturing optical materials; and the double-mesopore silicon dioxide transparent gel monolith without the surfactant can be widely applied to catalysis and separation.

The invention discloses a method for preparing molybdenum sulfide with a three-dimensional hollow structure based on upconversion nanoparticles. The specific method includes: first preparing up-conversion nanoparticles with regular shape and uniform size distribution; Sodium and ammonium tetrathiomolybdate, stir until uniform; transfer the mixture to a reaction kettle, react at 220°C for 12 hours, then cool to room temperature, and finally form upconversion particles @MoS 2 Composite material; at room temperature, the upconversion nanoparticles are etched with dilute hydrochloric acid to obtain a three-dimensional hollow structure MoS 2 . This method has the characteristics of mild reaction conditions, convenient operation, low equipment requirements, low raw material cost, controllable product shape, high yield and easy mass production. application prospects.

The invention discloses an hsa-miR-9 (Human Serum Albumin-Micro Ribonucleic Acid-9) detection kit and an hsa-miR-9 detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.

The invention relates to microRNA, particularly an hsa-miR-363 detection kit based on AllGlo probe fluorescence quantitative PCR (polymerase chain reaction) and a detection method thereof. The kit is provided with a box body, partitions, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescence quantitative PCR reagent bottle. The method comprises the following steps: extracting miRNA (microribonucleic acid) in a sample, wherein if the sample is serum / plasma or any other body fluid, after the sample is sufficiently cracked, 5 mu L of 5n mol exogenous reference cel-miR-39 provided by the kit is added and vortex oscillation is performed, and if the sample is a cell or tissue sample, no exogenous reference cel-miR-39 is needed; carrying out real-time PCR amplification on the stem-loop reverse transcription reagent reverse transcription miRNA provided by the kit as cDNA (omplementary deoxyribonucleic acid) by using the real-time fluorescence quantitative PCR reagent provided by the kit; and setting reasonable thresholds and base lines according to data given by a comprehensive analysis instrument, and carrying out result analysis.

The invention relates to microRNA, particularly an hsa-miR-520a-3p detection kit based on AllGlo probe fluorescence quantitative PCR (polymerase chain reaction) and a detection method thereof. The kit is provided with a box body, partitions, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescence quantitative PCR reagent bottle. The method comprises the following steps: extracting miRNA (microribonucleic acid) in a sample, wherein if the sample is serum / plasma or any other body fluid, after the sample is sufficiently cracked, 5 mu L of 5n mol exogenous reference cel-miR-39 provided by the kit is added and vortex oscillation is performed, and if the sample is a cell or tissue sample, no exogenous reference cel-miR-39 is needed; carrying out real-time PCR amplification on the stem-loop reverse transcription reagent reverse transcription miRNA provided by the kit as cDNA (omplementary deoxyribonucleic acid) by using the real-time fluorescence quantitative PCR reagent provided by the kit; and setting reasonable thresholds and base lines according to data given by a comprehensive analysis instrument, and carrying out result analysis.

The invention provides supermolecule phospholipid based on nucleic acid bases, a preparation method of the supermolecule phospholipid and a liposome comprising the supermolecule phospholipid. The supermolecule phospholipid provided by the invention comprises a hydrophilic phospholipid head and a hydrophobic phospholipid tail, wherein the hydrophilic phospholipid head is connected with the hydrophobic phospholipid tail by nucleic acid bases capable of being complementarily identified. Compared with the prior art, the supermolecule phospholipid based on the nucleic acid bases, which is provided by the invention, is formed by simply mixing of the hydrophilic phospholipid head and the hydrophobic phospholipid tail, and the hydrophilic phospholipid head and the hydrophobic phospholipid tail are connected together by molecular identification of the nucleic acid bases, i.e. the supermolecule phospholipid is formed by connecting of multiple hydrogen bonds between the complementary bases; the supermolecule phospholipid can be further assembled in water to form the liposome; due to sensitivity of the hydrogen bonds on the subacid environment, the liposome can be rapidly dissociated under the acid condition, so as to achieve the purpose of rapidly releasing a load.

The invention discloses an hsa-miR-146 (Human Serum Albumin-Micro Ribonucleic Acid-146) detection kit and an hsa-miR-146 detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.

The invention discloses a method for preparing hollow silicon dioxide microspheres, which comprises the following steps of: adding absolute ethyl alcohol and water into a beaker; uniformly stirring the absolute ethyl alcohol and water, then adding ammonia water into the mixture and keeping on stirring the mixture for 5 to 30 min; then adding tetraethoxysilane into the mixture and then quickly adding polystyrene microspheres within 1 to 5min; then stirring the mixture for reaction for 1 to 3h at normal temperature; filtering and washing the mixture after the reaction; drying the filter cake first at normal temperature and then at the temperature of between 500 and 600 DEG C; preserving the heat for 5 to 8h; and finally cooling the reaction product. The hollow SiO2 microspheres can be directly prepared by reacting unmodified PS microspheres at normal temperature so as to simplify the synthesis process and ensure simple preparation process and easy control and implementation; the nanoparticles of the formed hollow microspheres have micropores of less than 2nm; and mesopores and macropores of dozens of nanometers are formed among the nanoparticles; therefore, the multilayer nanoporousstructure contributes to the adsorption and transport of materials.

The invention discloses an hsa-miR-513b (Human Serum Albumin-Micro Ribonucleic Acid-513b) detection kit and an hsa-miR-513b detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.