55results about How to "The synthesis procedure is simple" patented technology

The invention discloses a double-mesopore silicon dioxide transparent gel monolith and a preparation method thereof, which belong to the technical field of inorganic porous materials. The invention particularly relates to a large-size, flawless and transparent silicon dioxide gel monolith with double-mesopore distribution characteristics and a fast preparation method thereof. The silicon dioxide gel monolith is characterized in that the double-mesopore pore-size distribution of the silicon dioxide gel monolith is in a mesoporous region, and is specifically characterized in that: the double-mesopore silicon dioxide transparent gel monolith is provided with the combination of a cylindrical primary mesopore and a worm-like secondary mesopore, and the N2 adsorption isotherms of the cylindrical primary mesopore and the worm-like secondary mesopore are type IV curves having characteristics of a type H1 hysteresis loop. The preparation method has a short synthetic cycle and simple operating steps and is easy to control, the prepared double-mesopore silicon dioxide transparent gel monolith containing a surfactant has a regular shape, a controllable size and high thermal stability and transparency, and is suitable for manufacturing optical materials; and the double-mesopore silicon dioxide transparent gel monolith without the surfactant can be widely applied to catalysis and separation.

The invention discloses a method for preparing molybdenum sulfide with a three-dimensional hollow structure based on upconversion nanoparticles. The specific method includes: first preparing up-conversion nanoparticles with regular shape and uniform size distribution; Sodium and ammonium tetrathiomolybdate, stir until uniform; transfer the mixture to a reaction kettle, react at 220°C for 12 hours, then cool to room temperature, and finally form upconversion particles @MoS 2 Composite material; at room temperature, the upconversion nanoparticles are etched with dilute hydrochloric acid to obtain a three-dimensional hollow structure MoS 2 . This method has the characteristics of mild reaction conditions, convenient operation, low equipment requirements, low raw material cost, controllable product shape, high yield and easy mass production. application prospects.

The invention discloses an hsa-miR-9 (Human Serum Albumin-Micro Ribonucleic Acid-9) detection kit and an hsa-miR-9 detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.

The invention relates to microRNA, particularly an hsa-miR-520a-3p detection kit based on AllGlo probe fluorescence quantitative PCR (polymerase chain reaction) and a detection method thereof. The kit is provided with a box body, partitions, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescence quantitative PCR reagent bottle. The method comprises the following steps: extracting miRNA (microribonucleic acid) in a sample, wherein if the sample is serum / plasma or any other body fluid, after the sample is sufficiently cracked, 5 mu L of 5n mol exogenous reference cel-miR-39 provided by the kit is added and vortex oscillation is performed, and if the sample is a cell or tissue sample, no exogenous reference cel-miR-39 is needed; carrying out real-time PCR amplification on the stem-loop reverse transcription reagent reverse transcription miRNA provided by the kit as cDNA (omplementary deoxyribonucleic acid) by using the real-time fluorescence quantitative PCR reagent provided by the kit; and setting reasonable thresholds and base lines according to data given by a comprehensive analysis instrument, and carrying out result analysis.

The invention discloses an hsa-miR-146 (Human Serum Albumin-Micro Ribonucleic Acid-146) detection kit and an hsa-miR-146 detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.