145results about How to "PH sensitive" patented technology

The invention relates to in-situ crosslinked alginate hydrogels with an active ingredient of disulfide bond disulfide bond alginate derivative. The preparation method thereof is as follows: the organic liquids of carbodiimide hydrochloride and N-hydroxysuccinimide eater are added into the alginate aqueous liquid of the alginate to activate the carboxyl of the alginate aqueous liquid; then the organic liquid of 4-aminothiolphenol is added under the conditions of keeping in dark place under 10 DEG C and nitrogen protection, so that the amino of the 4-aminothiolphenol and the carboxyl of the alginate aqueous liquid form an amido bond. The proportions of each matter adjusted and the pH value of muriatic acid is adjusted to 6.0, ethanol is deposited, frozen and dried to obtain sulfhydrylation alginates of different sulfydryl contents. The alginate aqueous liquid of the alginate oxidizes the sulfydryl thorugh the oxygen in the liquid under a room temperature to crosslink and form a disulfide bond, thus obtaining the crosslinked alginate hydrogels. The hydrogels prepared through the method has pH sensitivity and reduction responsiveness, and has potential application values in drug delivery field.

The invention discloses pH response four-arm star block copolymer and a preparation method and application thereof. According to the copolymer, pentaerythritol is used as an inner core, each arm is connected with a hydrophobic group, a pH response group and a hydrophilic group; and the preparation method comprises the following steps of: performing ring opening polymerization on pentaerythritol to obtain hydrophobic group copolymer, performing acylation on the tail end of the hydrophobic group copolymer, and sequentially initiating pH response monomer and the hydrophilic group to perform electron transfer activating agent regeneration-atom transfer radical polymerization by using hydrophobic group copolymer of which the tail end is subjected to acylation and which is used as macroinitiator to obtain the pH response four-arm star block copolymer. The copolymer is self-assembled into nano micelle in an aqueous solution, so that insoluble medicines can be effectively solubilized; the copolymer is used for preparing a micelle medicine carrying system for insoluble anti-cancer medicines, and the medicines can be kept to be slowly released when pH of normal tissues is 7.4, and can be quickly released in a controllable way under the faintly acid condition that pH of cancer cells is 5 to 6; and the proportion of foundational groups of the copolymer is easy to control, and the copolymer is simple in synthesis process and high in yield.

The invention belongs to the fields of high polymer materials and biomedical engineering and particularly relates to a method for preparing pH-responsive graft copolymer taking ethyl cellulose as a main chain. The method comprises the following steps of: performing esterification reaction on residual hydroxyl in the ethyl cellulose main chain and a bromine-containing compound under the protection of inert gas nitrogen or argon to convert the hydroxyl into a bromine group; and performing atom transfer radical polymerization on a methacrylic acid-N,N-lignocaine ethyl ester monomer by using the bromine group as a macroinitiator to form pH-responsive polymer so as to finally obtain the pH-responsive graft copolymer taking the ethyl cellulose as the main chain. The pH-responsive graft copolymer taking the ethyl cellulose as the main chain has biodegradability, biocompatibility and pH value sensitivity and can be self-assembled into stable nano micelles in water, so the pH-responsive graft copolymer can be widely applied to the fields such as medicament release control carriers, biological intelligent switches, biological sensors, biological nano devices and the like. The method has the advantages of simpleness, practicability, industrialized production of raw materials and high popularization and application value.

The invention discloses a pH-responsive 6-arm star block copolymer and a preparation method and application thereof. In the structure of the copolymer, pentaerythritol is adopted as a kernel, and each arm is connected with a hydrophobic group, a pH-responsive group and a hydrophilic group. The preparation method comprises the steps of: carrying out ring opening polymerization of the pentaerythritol to obtain a hydrophobic group polymer, carrying out acylation of the end of the hydrophobic group polymer to prepare a macromolecular initiator, carrying out sequential initiation of regeneration of an electron transfer activator for a pH-responsive monomer and the hydrophilic group, i.e. radical polymerization for atom transfer, thereby obtaining the pH-responsive 6-arm star block copolymer. The polymer is self-assembled into nano micelles in aqueous solution, the dissolution of water-insoluble drugs can be enhanced effectively, the polymer can be applied in preparation of a micelle carrying system for water-insoluble anticancer drugs, and the drugs can be released slowly when the pH of normal tissues is 7.4 and rapidly in a controlled way under acidic conditions when the pH of tumor cells is 5-6; and the proportion of functional groups can be easily adjusted, the synthesis process is simple, and the yield is higher.

The invention belongs to the technical field of high polymer materials, and relates to a sensible hydrogel, in particular to a hydrogel with pH sensibility and temperature sensibility and a preparation method thereof. The preparation method comprises the following steps: synthesizing hydroxy propyl cellulose and acrylic acid or acryloyl chloride into acrylic acid cellulose ester and copolymerizing the prepared acrylic acid cellulose ester and acrylic acid monomer to form the hydrogel. The hydrogel comprises hydroxy propyl cellulose chain segment with pH sensibility and temperature sensibility and acrylic acid chain segment with pH sensibility, and meanwhile, the hydrogel has pH sensibility, temperature sensibility and degradability and flexible responsiveness to the pH, can rapidly generate volume expansion or contraction, and has favorable mechanical property, good intellectuality and easy and feasible synthetic method, and the temperature sensibility is influenced by the pH value of the solution.

The invention provides preparation of a keratin-sodium alginate composite microporous gel, belonging to the fields of composite materials and biotechnology. The preparation method comprises the following step: carrying out self-crosslinking polymerization on the raw materials biocompatible natural high-polymer protein and polysaccharide (keratin and sodium alginate) under the actions of an organic crosslinking agent, an inorganic crosslinking agent an initiator. The composite microporous gel has favorable swelling and deswelling properties, is sensitive to pH value, and has slow-release effects on both low-molecular and high-molecular drugs. The in-vitro drug release performance experiment indicates that the composite microporous gel can implement controlled release of drug molecules by utilizing the acid sensitiveness, and thus, can be used as a drug carrier in drug controlled release.

The invention relates to a pH-sensitive anticancer prodrug and a preparation method as well as application thereof, which belongs to the field of medicine and chemical industry. The technical problem to be solved is to provide the pH-sensitive anticancer prodrug, and the anticancer prodrug has highest solubility when the pH value is between 4 and 6, can nearly not be dissolved when the pH value is less than 3 or more than 7, and almost does not release adriamycin during the circulation due to small solubility particularly when circulating in blood (the pH value is approximately equal to 7.4), thereby having small toxic side effect. The pH-sensitive anticancer prodrug is a conjugate obtained by coupling the adriamycin and pectin with a molecular weight of between 71,000 and 400,000 through a chemical bond. The pH-sensitive anticancer prodrug is obtained by the following steps: a pectin aqueous solution and an adriamycin aqueous solution are added with a dehydrating agent to react under the condition that the pH value is between 6 and 8, and then are dialyzed and condensed.

The invention relates to a preparation method for a starch-based microgel and belongs to the technical field of starch deep processing. By taking pH sensitive carboxymethyl starch and beta-cyclodextrin with an embedding characteristic as raw materials, a carboxymethyl starch/beta-cyclodextrin compound microgel is prepared by virtue of an inverse emulsion polymerization method. The morphological structure of the target starch-based microgel prepared by the method provided by the invention is spherical and the grain size distribution is 10-20 [mu]m. The microgel has pH sensitivity, hydrophobic embedding property and good biocompatibility and biodegradability, and has wide use in the field of foods, medicines and the like.

The invention discloses a preparation method of a multi-responsive azobenzene functionalized polymer. The preparation method comprises the following steps: synthesizing a polymer of poly-dimethylaminoethyl (meth)acrylate by virtue of atom transfer radical polymerization reaction of a polymer monomer of dimethylaminoethyl methacrylate; synthesizing azobenzene small-molecule m-trifluoromethylazophenol with phenolic hydroxyl by virtue of diazo reaction by taking m-trifluorotoluidine and phenol as raw materials; then performing nucleophilic substitution with dibromobutane to generate azobenzene small-molecule 3-trifluoromethyl-4-brombutylazobenzene containing active bromine at one end; and performing quaterisation at 35 DEG C to graft azo groups to poly-dimethylaminoethyl (meth)acrylate to finally obtain azobenzene functionalized poly-dimethylaminoethyl methacrylate. The synthetic method disclosed by the invention is simple and feasible, the reaction temperature is lower than the conventional reaction temperature, and the synthesized polymer not only has temperature responsive property and pH responsive property but also has light responsive property, so that the polymer is a novel polymer with temperature, pH and light responsive properties.

The invention discloses a synthesis method of a water-soluble squarylium indocyanine multifunctional cell fluorescent dye. The dye is prepared through multi-step chemical synthesis, and finally, squarylium indocyanine contains cationic amine functional groups, thereby improving the water solubility and biocompatibility of the squarylium cyanine. The fluorescent molecule can interact with DNA (deoxyribonucleic acid) in cell nuclei or phospholipid molecules in cell membranes, thereby specifically marking cell membranes of living cells and cell nuclei of fixed/dead cells. By combining the two cell marking characteristics, the fluorescent dye can successfully mark cell nuclei of cells which are being subjected to apoptosis in living tissues. According to the invention, the method is mature in synthesis process and simple to operate; and the product has the advantages of safety, no toxicity, high molecule designability, excellent water solubility, favorable light stability, high pH-sensitivity and the like, and can be used as a multifunctional cell fluorescent marking molecule in scientific study, diagnosis and production in the biomedicine field.

The invention relates alginic acid inorganic nanometer composite gel microspheres and a preparation method; the gel microspheres comprise the following components by weight percent: 1-7% of alginate, 0.5-10% of carbon nanotubes, 1-50% of triblock surfactant polyethylene oxide-polypropylene oxide-polyethylene oxide and the balance water. The production process of the gel microspheres is safe, simple and effective and accords with the requirements of environmental protection. The alginic acid carbon nanotubes composite gel microspheres which is prepared by the preparation method of the invention and is introduced with carbon nanotubes can relieve the swelling speed of the complexed alginic acid microspheres, can be used as a carrier to combine with drugs, proteins, enzymes and other biological products and can be used as a drug carrier owning to the pH sensitivity of the composite gel microspheres.

The invention relates to a method for preparing a pH sensitive hydrogel which has an excellent adsorbing capability on lead, cadmium, nickel and other heavy metal ions and is large in adsorption quantity, high in adsorption efficiency and sensitive to pH value of a solution, as well as a product of the pH sensitive hydrogel. The hydrogel is prepared by the following steps: washing attapulgite with water and acid; modifying the attapulgite by utilizing tannic acid; compounding with acrylic acid hydrogel. The method for preparing the adsorption type pH sensitive hydrogel has the characteristics of simple process, low cost, no special equipment and the like, and is easy for realization of industrial implementation, and the strength of the prepared gel is greatly improved. The gel can be applied to sewage treatment fields, such as electroplating industry, chemical industry and the like, can control the adsorption quantity of heavy metal ions by controlling the pH value of a solution, and can be washed and recycled.

The invention discloses a doxorubicin prodrug, its preparation method and an injectable composition. The doxorubicin prodrug is formed by hydrazone key-type connection between MPEG and DOX. The preparation method of the doxorubicin prodrug is simple; requirements on reaction conditions are low; yield is high; and production costs are easier to control. The prodrug has low immunity and can effectively raise water solubility of doxorubicin. In addition, it will take a long time to remove the doxorubicin prodrug in vivo such that action time of the drug can be effectively prolonged. The doxorubicin prodrug has pH sensitivity and can release doxorubicin in situ in an acidic pH environment and simultaneously form PEG. The doxorubicin prodrug composition can be dissolved in an approximately-neutral aqueous solution to form an aqueous solution with stable performance. When pH changes to acid, supermolecule hydrogel can be formed within minutes. Long-acting in situ release of doxorubicin can be achieved. Doxorubicin diffusion can be effectively avoided. Functions of normal cells are influenced. Toxic and side effect of the drug is reduced.

The invention discloses a preparation method of a pH sensitiveness raphanin chitosan microsphere. The preparation method comprises the following steps: 1) dissolving chitosan powder into an acetic acid solution; 2) taking a raphanin water solution and adding into the acetic acid solution; and 3) dropping a sodium tripolyphosphate solution into the mixture liquid obtained in the step 2) so as to obtain the raphanin-chitosan microsphere. The preparation method of the pH sensitiveness raphanin chitosan microsphere is reasonable in design, and the microsphere is prepared by using an ionic cross-linking method in a mode that the chitosan is taken as a carrier and the sodium tripolyphosphate is taken as a cross-linking agent, the experiment is simple and mild, and no organic solvents are used, so that the method is safe and environmental-friendly, a drug carrying microsphere solution of which the average particle size is 500 nm is successfully prepared, and moreover the prepared microsphere solution is good in stability and has no great change in particle size after being placed for a long time; under the acidic condition, the raphanin can be released more easily, and the higher the pH value is, the longer the releasing time is, and therefore the microsphere is an ideal slow release formulation of the raphanin.

The invention discloses a preparation method of modified carboxymethyl chitosan nano gel, belonging to the technical field of carriers and sustained-release material. The preparation method comprises the following steps: firstly preparing methylacryloyl carboxymethyl chitosan; and performing free radical polymerization in the presence of N'-N-bis(acrylyl) cystamine serving as a cross-linking agent to prepare the modified carboxymethyl chitosan nano gel. Through determining the particle size and zeta potential of nano particles under the condition of different pH values, the modified carboxymethyl chitosan nano gel is proved to have a pH sensitivity. A protein adsorption test shows that the nano gel is capable of preventing protein adsorption. The prepared nano gel is used for successfully carrying anticancer drug doxorubicin hydrochloride; in-vitro release tests in which PBS solutions different in pH and glutathione concentration are used as media prove that the drug-carried nano particles have relatively good pH and reduction sensitivity.

The invention belongs to the field of macromolecular nano biological medicine materials, and in particular relates to a high-efficiency and antibacterial polymeric micelle with high pH sensibility and biocompatibility and a preparation method thereof. An amphipathic block polymer is subjected to self-assembly by a direct dissolution method to form the micelle; and the surface of the micelle has a large number of positive charges, so that the micelle has the effects of sterilizing and inhibiting bacteria in high efficiency under the condition that other antibiotics are not added. Simultaneously, the micelle has high biocompatibility and biodegradability. The polymeric micelle has high pH sensibility, and a structure of the polymeric micelle is changed with the change of the outside pH value, and multiple medicines can be wrapped by a core-shell structure of the micelle, so the polymeric micelle has a bright application prospect.

The invention relates to a preparation method of a pH response type multi-scale structure polyvinylidene fluoride (PVdF) nanofiber membrane. The nanofiber membrane is obtained by conducting copolymerization on a PVdF spinning solution treated with acrylic acid and polyethylene polyamine and then performing electrostatic spinning. The preparation method is characterized in by comprising the four steps of 1 PVdF solution preparation, 2 solvent curing, 3 graft copolymerization with the acrylic acid and 4 electrostatic spinning. The pH response type multi-scale structure polyvinylidene fluoride (PVdF) nanofiber membrane prepared by means of the preparation method has sensitive pH response characteristics and has a broad application prospect in the fields of chemical or biological separation, substance analysis and detection, separation and purification of precious metals and other fields.

The invention relates to an application of a pH-sensitive coaxial polylactate caprolactone PLCL/gelatin double drug-loaded fiber scaffold, namely the application of the pH-sensitive coaxial polylactate caprolactone PLCL/gelatin double drug-loaded fiber scaffold as a tissue engineering scaffold. The fiber scaffold with a drug controlled release function has good biocompatibility, is degradable, has pH sensitivity and good mechanical property and can play roles in diminishing inflammation and resisting cancers.

The invention discloses a method for preparing a honeycomb silk fibroin porous microsphere sustained drug release vector. Fibrous fibroin formed after cocoon degumming is dissolved, filtered, dialyzed and concentrated to obtain silk fibroin aqueous solution; the silk fibroin aqueous solution is subject to ultrasonic dispersion, placed in a dialysis bag and placed in simulated human body fluids of different times concentrations to be soaked, the silk fibroin aqueous solution is processed in a uniform shaking table, the simulated human body fluids are replaced at time intervals to obtain microsphere suspension; and the microsphere suspension is washed many times and undergoes centrifugal separation and drying to obtain apatite/silk fibroin microsphere powder as the sustained drug release vector. Apatite microspheres of a certain size and pore diameters can be formed on the surface of silk fibroin, the dispersity, drug loading performance and sustained release performance are excellent, the honeycomb silk fibroin porous microsphere sustained drug release vector has no toxic or side effect on normal cells, the biocompatibility of the drug vector is improved remarkably, and the honeycomb silk fibroin porous microsphere sustained drug release vector has wide application prospects in the fields such as sustained drug release, tissue engineering and enzyme engineering.

The invention discloses a pH-sensitive konjac glucomannan-lipidosome compound nano drug carrier for injection, preparation and application thereof, and belongs to the technical field of biological materials and slow release. The pH-sensitive konjac glucomannan-lipidosome compound nano drug carrier is characterized in that hydrophobic modified konjac glucomannan and lipidosome are compounded and assembled by an ethanol injection method and a thin-film hydration method and the like, the obtained nano drug carrier has higher encapsulation efficiency for both hydrophilic and hydrophobic drugs, andcan be loaded with different hydrophilic and hydrophobic drugs to realize synergistic transfer. The nano drug carrier can be enriched to tumor tissues by EPR (Enhanced Permeability and Retention) effect, due to the pH sensitivity, the drugs can be selectively released in the tumor tissues, and in normal tissues and blood, the physical and chemical stability of the entrapped drugs are obviously improved. The drug-loaded KGM-LIPO compound nano drug carrier is stable in structure, simple in preparation method, good in biocompatibility and low in cytotoxicity, has an obvious effect in killing tumor cells, and can be used in the fields of drug control release and synergistic treatment and the like.

The invention provides a preparation method of self healing hydrogel with ultraviolet and pH sensitive. The method comprises the following steps of 1, preparing azobenzene polymerizable monomers; forming subject-object inclusions by cyclodextrin and the azobenzene polymerizable monomers; 2, preparing phenylboronic acid polymerizable monomers; 3, forming crosslinking points under the basic condition through the reaction between the phenylboronic acid polymerizable monomers and cis-diol on cyclodextrin inclusions and performing liquid separation by a liquid separation funnel to obtain yellow emulsion at the lowermost layer which is the formed azobenzene cyclodextrin inclusions; 4, adding oxidization-reduction initiators, crosslinking agents and water-soluble monomers at the room temperatureto obtain the hydrogel with the ultraviolet and pH response performance and self healing performance. The simple synthesis steps are used; the physical non-covalency subject-object inclusion effect and the chemical covalency reversible boric acid ester bonds are used; the hydrogel with self healing function, pH sensitivity, high mechanical property and high stability is formed.

The invention relates to a preparation method of a 5-fluorouracil-carried pH-sensitive drug-bearing microsphere, which is characterized in that: monomer sodium styrene sulfonate is put into a cross-linked polyvinyl alcohol microsphere after being soaked, then ceric ammonium sulfate and concentrated sulfuric acid are added to obtain a grafted microsphere CPVA-g-PSSS, 5-fluorouracil is dissolved inhydrochloric acid, and the grafted microsphere CPVA-g-PSSS prepared in the previous step is added to obtain the 5-fluorouracil-carried pH-sensitive drug-bearing microsphere. The drug-bearing microsphere has pH sensitivity, is basically not released in stomach and small intestines and is greatly released in colon. Due to adoption of the preparation method, the treatment effect of the 5-fluorouracil can be improved, the selectivity is improved, the dosage can be reduced, and the toxic side effect can be reduced.

The invention discloses an anionic-sodium-alginate-based production method of a pH-responsive intestine-targeted active factor carrying system, and belongs to the technical field of production of microcapsules. According to the anionic-sodium-alginate-based production method of the pH-responsive intestine-targeted active factor carrying system, with anionic polysaccharide sodium alginate as a wallmaterial, by adopting a microfluidic technology, by means of an in-situ inner and outer gel method, an active factor procyanidine is coated, then through layer-by-layer self-assembly, the surface ofcalcium alginate is modified with chitosan to form the pH-responsive intestine-targeted active factor carrying system with certain strength, uniform size and a shell-core structure, the stability of the procyanidine can be significantly improved, the procyanidine is protected from effects of factors of an external environment, a gastric fluid strong acid and the like, the procyanidine is transported to an intestinal tract part for release, the bioavailability of the procyanidine is improved, a novel idea is provided for development of the novel multifunctional active factor carrying system, and application of the novel multifunctional active factor carrying system in the field of food is promoted. The anionic-sodium-alginate-based production method of the pH-responsive intestine-targeted active factor carrying system is simple to operate, used materials are low in cost, embedding conditions are mild, and large-scale production is easy.

A preparation method of a compound water gel material comprises the following steps of respectively dissolving two kinds of monosaccharide or oligosaccharide compounds in organic solvent; adding catalyst and glycidyl methacrylate in the organic solvent; performing reaction for 20-30 hours at the temperature of 40-60 DEG C to obtain carbohydrate reaction liquors acylated by methacrylonitrile respectively; mixing the carbohydrate reaction liquors after the carbohydrate reaction liquors are cooled down to room temperature; adding crylic acid, acrylamide and cross-linking agents in the carbohydrate reaction liquors; adding water in the mixture so that the crylic acid, the acrylamide and the cross-linking agents can be dissolved completely; heating the mixture in water bath until the temperature is 50 DEG C; keeping warm for half hour; adding initiating agents in the mixture; performing stirring reaction in a thermostat water bath for 0.5-5 hours at the temperature of 50-80 DEG C to obtain gel; taking out the gel after reaction is finished; cleaning the gel by using deionized water; cutting off the gel into small pieces; soaking the small pieces of the gel in the deionized water for 7 days; changing water at intervals of 6 hours; and performing freeze drying on products to obtain the ccompound water gel material. The gel has high application value on the aspect of wastewater treatment of a textile industry, a chemical industry and the like.