In-situ crosslinked alginate hydrogels and preparation method thereof

A technology of sodium alginate and in-situ cross-linking, which is applied to pharmaceutical formulations, medical preparations of non-active ingredients, aerosol delivery, etc., to achieve the effects of easy preparation, good biocompatibility, and convenient dosage form molding

Inactive Publication Date: 2012-04-11
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an in-situ cross-linked sodium alginate hydrogel and its preparation method to solve the deficiencies and defects of the prior art

Method used

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  • In-situ crosslinked alginate hydrogels and preparation method thereof
  • In-situ crosslinked alginate hydrogels and preparation method thereof
  • In-situ crosslinked alginate hydrogels and preparation method thereof

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preparation example Construction

[0030] The preparation method of the in-situ cross-linked sodium alginate hydrogel provided by the present invention specifically adopts the following method to prepare the in-situ cross-linked sodium alginate hydrogel, and the steps include:

[0031] (1) Preparation of thiolated sodium alginate:

[0032] According to the proportion, add the organic solution of carbodiimide hydrochloride and N-hydroxysuccinimide to the sodium alginate aqueous solution, after stirring evenly, add the organic solution of p-mercaptoaniline dropwise under the condition of low temperature, dark and nitrogen protection. Continue to stir for 1-24 hours. After the reaction, adjust the pH value of the reaction system to 6, add ethanol to precipitate and freeze-dry to obtain thiolated sodium alginate with different thiol content; the low temperature is ≤10°C.

[0033] (2) Preparation of in situ cross-linked sodium alginate hydrogel:

[0034] According to the proportion, the aqueous solution of thiolate...

Embodiment 1

[0037] Dissolve 0.15 g of sodium alginate in 5 mL of deionized water to obtain a 3% sodium alginate aqueous solution by weight, add 1 mL of carbodiimide hydrochloride and N-hydroxysuccinimide in dimethyl sulfoxide (seaweed The molar ratio of sodium acid sugar unit to carbodiimide hydrochloride is 1:1.1, and the molar ratio of carbodiimide hydrochloride to N-hydroxysuccinimide is 1:1), stir well, and avoid Add 1 mL of 9% (W / V) dimethyl sulfoxide solution of p-mercaptoaniline dropwise under light and nitrogen protection conditions, stir for 5 hours, adjust the pH value to 6 with 0.1M hydrochloric acid, add ethanol to precipitate, and then Wash twice with a small amount of ethanol, and freeze-dry to obtain mercaptolated sodium alginate with a mercapto group content of 586.1 μmol mercapto group / g polymer.

[0038]Dissolve 0.15g of thiolated sodium alginate in 5mL of deionized water to obtain a 3% (W / V) aqueous solution of thiolated sodium alginate, then pour it into the mold, and ...

Embodiment 2

[0040] Dissolve 0.10 g of sodium alginate in 5 mL of deionized water to obtain a 2% sodium alginate aqueous solution, add 1 mL of carbodiimide hydrochloride and N-hydroxysuccinimide in dimethyl sulfoxide (seaweed The molar ratio of sodium acid sugar unit to carbodiimide hydrochloride is 1:1.1, and the molar ratio of carbodiimide hydrochloride to N-hydroxysuccinimide is 1:1), stir well, and avoid Add 1 mL of 5% (W / V) dimethyl sulfoxide solution of p-mercaptoaniline dropwise under light and nitrogen protection conditions, stir and react for 5 hours, adjust the pH value to 6 with 0.1M hydrochloric acid, add ethanol to precipitate, and then Wash twice with a small amount of ethanol, and freeze-dry to obtain mercaptolated sodium alginate with a mercapto group content of 470.7 μmol mercapto group / g polymer.

[0041] Dissolve 0.10g of mercaptolated sodium alginate in 5mL of deionized water to obtain a 2% (W / V) mercaptolated sodium alginate aqueous solution, then pour it into the mold...

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Abstract

The invention relates to in-situ crosslinked alginate hydrogels with an active ingredient of disulfide bond disulfide bond alginate derivative. The preparation method thereof is as follows: the organic liquids of carbodiimide hydrochloride and N-hydroxysuccinimide eater are added into the alginate aqueous liquid of the alginate to activate the carboxyl of the alginate aqueous liquid; then the organic liquid of 4-aminothiolphenol is added under the conditions of keeping in dark place under 10 DEG C and nitrogen protection, so that the amino of the 4-aminothiolphenol and the carboxyl of the alginate aqueous liquid form an amido bond. The proportions of each matter adjusted and the pH value of muriatic acid is adjusted to 6.0, ethanol is deposited, frozen and dried to obtain sulfhydrylation alginates of different sulfydryl contents. The alginate aqueous liquid of the alginate oxidizes the sulfydryl thorugh the oxygen in the liquid under a room temperature to crosslink and form a disulfide bond, thus obtaining the crosslinked alginate hydrogels. The hydrogels prepared through the method has pH sensitivity and reduction responsiveness, and has potential application values in drug delivery field.

Description

technical field [0001] The invention relates to the field of gel materials, in particular to a preparation method of in-situ crosslinked sodium alginate hydrogel. Background technique [0002] Sodium alginate is extracted from kelp or sargassum of brown algae. It is a sodium salt of polyanionic polysaccharide composed of β-D-mannuronic acid and α-L-guluronic acid. It is non-toxic and has good Biocompatible, biodegradable and non-immunogenic. There are a large number of carboxyl and hydroxyl groups in the structure of sodium alginate, which can be modified by various grafting reactions such as chemical grafting, chemical-enzymatic grafting and ultraviolet light grafting. [0003] Hydrogel is the most common and important substance that contains a lot of water but is insoluble in water. It can absorb water several times to hundreds of times its own weight in water and still maintain its shape. Polymer hydrogel is a common type of hydrogel. Its structure is a three-dimensiona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04C08J3/24C08J3/075A61K47/36A61K9/06
Inventor 殷以华常丹郑化孙艳娟
Owner WUHAN UNIV OF TECH
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