101results about How to "Wide range of potential uses" patented technology

The invention relates to in-situ crosslinked alginate hydrogels with an active ingredient of disulfide bond disulfide bond alginate derivative. The preparation method thereof is as follows: the organic liquids of carbodiimide hydrochloride and N-hydroxysuccinimide eater are added into the alginate aqueous liquid of the alginate to activate the carboxyl of the alginate aqueous liquid; then the organic liquid of 4-aminothiolphenol is added under the conditions of keeping in dark place under 10 DEG C and nitrogen protection, so that the amino of the 4-aminothiolphenol and the carboxyl of the alginate aqueous liquid form an amido bond. The proportions of each matter adjusted and the pH value of muriatic acid is adjusted to 6.0, ethanol is deposited, frozen and dried to obtain sulfhydrylation alginates of different sulfydryl contents. The alginate aqueous liquid of the alginate oxidizes the sulfydryl thorugh the oxygen in the liquid under a room temperature to crosslink and form a disulfide bond, thus obtaining the crosslinked alginate hydrogels. The hydrogels prepared through the method has pH sensitivity and reduction responsiveness, and has potential application values in drug delivery field.

The invention discloses a preparation method and application of an electrochemical malathion biosensor based on a bi-metal in-situ composite two-dimensional nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel flaky magnetic nano light-sensitive material FeMn-N@TiO2 is prepared for the first time; through good bio-compatibility, large specific area and ferromagnetism of the material, a malathion antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects malathion is prepared.

The invention discloses a preparation method of a photoelectrochemical diethylstilbestrol sensor and belongs to the technical field of novel nano functional materials and biosensors. Firstly, a novel two-dimensional nano photoelectric material, namely a copper-doped nano photoelectric material, specifically a two-dimensional nano composite Cu-TiO2 / MoS2 obtained through in-situ compounding of molybdenum disulfide on a copper-doped titanium dioxide nano cube is prepared, and an diethylstilbestrol antibody is loaded and alkaline phosphatase is fixed to the material by the application of good biocompatibility and a large specific surface area of the material. During detection, due to the fact that alkaline phosphatase can catalyze L-ascorbic acid-2-trisodium phosphate AAP to generate L-ascorbic acid AA in situ and then provide an electron donor for photoelectric detection, by the application of the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the photoelectric current intensity is lowered accordingly, and finally the photoelectric sensor for detecting diethylstilbestrol through an unmarked photoelectrochemical method is constructed.

The invention discloses a photoelectrochemical sensor for estradiol based on a boron-doped iron cobalt oxide two-dimensional nano composite material as well as a preparation method and application of the photoelectrochemical sensor. The preparation method comprises the following steps: firstly, modifying a two-dimensional nano material g-C3N4 on ITO (Indium Tin Oxide) conductive glass by adopting an electrodeposition method; secondly, carrying out in-situ grown of iron cobalt oxide by adopting a hydrothermal method, and further preparing to obtain a working electrode loaded with the g-C3N4, doped with boron and modified by the iron cobalt oxide Bi@Fe*Co<1->*O3 / g-C3N4; finally, loading an estradiol antibody by using good biocompatibility and large specific surface area of the material. During detection, L-ascorbic acid-2-trisodium phosphate (AAP) can be catalyzed by boron-doped iron cobalt oxide Bi@Fe*Co<1->*O3 to generate L-ascorbic acid AA in situ, further an electron donor is provided for photoelectric detection, and then photocurrent intensity is correspondingly reduced by using the influence of specific combination of the antibody and antigen on electronic transmission capacity; finally, the construction of the photoelectrochemical sensor for the estradiol by adopting a mark-free photoelectrochemical detection method is realized.

The invention discloses a carbon nitride-binary metal boron oxide composite material-based bisphenol A photochemical sensor and preparation and application thereof. The preparation comprises the steps of firstly modifying a two-dimensional nano material g-C3N4 on ITO conductive glass by adopting an electrodeposition method; growing nickel-cobalt hydroxide in situ by adopting a hydrothermal method to prepare a g-C3N4-loaded and boron-doped nickel-cobalt oxide Bi@NixCo<1-x>O3 / g-C3N4-modified working electrode; and finally loading a bisphenol A antibody by adopting good biocompatibility and large specific surface area of the material, thereby completing preparation of the bisphenol A photochemical sensor. When the detection is implemented, L-ascorbic acid-2-trisodium phosphate APP can be catalyzed by the boron-doped nickel-cobalt oxide Bi@NixCo<1-x>O3 to generate the L-ascorbic acid AA in situ, so that an electron donor is provided for photoelectric detection, the photocurrent strength is correspondingly reduced by utilizing the influence on the electronic transmission capability caused by the specificity quantitative combination of the antibody and antigen, and construction of a photoelectric sensor for detecting the bisphenol A by using an unmarked photoelectrochemical technique is finally achieved.

The invention belongs to the technical field of nano functional materials and green energy and discloses a preparation method of a two-dimensional nano-flake photocatalyst which is not doped with precious metal and is low in cost, simple to prepare and high in photocatalytic activity. The prepared two-dimensional nano-flake photocatalyst is manganese-doped titanium dioxide in-situ composite carbon nitride two-dimensional nanocomposite Mn-TiO2 / g-C3N4 and has good photocatalytic activity.

The invention discloses a preparing method of an electrochemistry parathion sensor based on a metal and metal oxide co-doped nanometer composite, and belongs to the technical field of novel nanometer functional materials and biosensors. The preparing method comprises the steps that firstly, a novel two-dimensional manometer material Co-MoO3 / TiO2@g-C3N4 is prepared, and a parathion antibody is loaded by using the good biocompatibility and the large specific area of the material; secondly, horse radish peroxidase is fixed through the cross-linking action of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide to generate electrochemistry signals, then the influence on the electron transmittability by the specific and quantitative combination of the antibody and an antigen is used, the current intensity is reduced correspondingly, and finally the parathion detecting electrochemistry biosensor low in cost, high in sensitivity and specificity, fast in detection and easy to prepare is obtained.

The invention discloses a preparing method for a photocatalyst free of precious metal doping, low in cost, easy to prepare and high in photocatalytic activity, and belongs to the technical field of novel nanometer functional materials and green energy.The binary metal co-doping photocatalyst prepared through the method is an iron-manganese co-doping titanium dioxide nanosheet in-situ synthesis carbon nitride two-dimensional nanocomposite FeMn-TiO2 / g-C3N4, and has good photocatalytic activity.

The invention discloses a magnetic belt material with a zero field cooling exchange bias effect. The chemical formula is Mn50CoNi49-xAlx, wherein x which refers to atomic percent content is larger than or equal to 10 and smaller than or equal to 15. The preparation method includes the steps: (1) weighing raw materials according to the chemical formula of Mn50CoNi49-xAlx; (2) containing the raw materials in a water cooling copper crucible, and preparing Mn50CoNi49-xAlx magnetic polycrystalline by means of the conventional electric arc melting method; and (3) using the smelted polycrystalline ingots to prepare the Mn50CoNi49-xAlx magnetic material by means of the rapid quenching method. Compared with existing alloy, the magnetic belt material has the advantages that the exchange bias effect under zero field cooling is achieved, and the magnetic belt material has higher magnetic field controllability, is low in cost and can be used for manufacturing of ultrahigh-density magnetic recording media, magnetic reading heads, giant magnetic resistors, spin valves, novel tunnel junction memories, sensors and the like.

The invention discloses a preparation method of an electrogenerated chemiluminescence sensor, and belongs to the technical field of novel nano-functional materials and biosensors. A novel two-dimensional nano-composite material is firstly prepared, manganese doped titanium dioxide nano-blocks are compounded with molybdenum disulfide in situ to form the two-dimensional nano-composite material Mn-TiO2 / MoS2, aflatoxin antibodies are loaded by the aid of excellent biocompatibility and large specific surface area of the material, manganese doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ when the aflatoxin is detected, electrochemical reaction of O2 and K2S2O8 in base solution are performed to generate electrogenerated chemiluminescence signals, electronic transmission capacity is affected by specific quantitative combination of the antibodies and antigen, so that current intensity is reduced, luminescent intensity is reduced, and the electrogenerated chemiluminescence sensor for detecting the aflatoxin by an unmarked electrogenerated chemiluminescence method is finally prepared.

The invention discloses a SiC / iron nitride nano composite material made from agricultural waste and a preparation method thereof. The SiC / iron nitride nano composite material made from agricultural waste comprises a core layer and a coating layer, wherein, the core layer comprises SiC powder, and the coating layer comprises iron nitride nanoparticles. The preparation method comprises following steps: first, processing the agricultural waste with pyrolysis or acid or alkali processing into silicon carbon powder having SiO2 and carbon as main composition, then processing the silicon carbon powder with thermite reaction or high-temperature reaction to obtain SiC, finally, coating a layer of iron nitride nanoparticles on the product with nitridation reaction to obtain the SiC / iron nitride nano composite material. The SiC / iron nitride nano composite material is porous and nano-sized structure, has high specific surface area and high electromagnetic loss property, and has a potential application in the fields of processing difficultly degraded effluent, absorbing materials and the like. The method disclosed in the invention has the advantages of low cost, simple technology, novel material structure, and wide potential application, and has huge application value.

The invention discloses a preparation method of a double-metal doped titanium dioxide polyhedral photocatalyst free of doped precious metals, low in cost, simple to prepare and high in photocatalytic activity and belongs to the technical fields of novel nano functional materials and green energy.The double-metal doped titanium dioxide polyhedral photocatalyst prepared by means of the preparation method is iron and cobalt double-metal in-situ compounded and nitrogen doped titanium dioxide nanosheets FeCo-N@TiO2 and has good photocatalytic activity.

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium-dioxide-based two-dimensional composite nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel two-dimensional flaky magnetic nano light-sensitive material FeCo-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a diethylstilbestrol antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects diethylstilbestrol is prepared.

The invention discloses a method for preparing a bimetallic co-doped two-dimensional nanoelectrode material which is free of precious metal doping, low in cost, easy to prepare and high in photoelectrochemical activity, and belongs to the technical field of novel nano functional materials and green energy. According to the method, an iron and manganese co-doped titanium dioxide nanosheet material is compounded on an iron and manganese co-intercalated molybdenum disulfide nanosheet in an in-situ mode, and a two-dimensional nano composite FeMn-TiO2 / MoS2 with an iron and manganese co-doped titanium dioxide nano diamond compounded on molybdenum disulfide in an in-situ mode is obtained. Prepared FeMn-TiO2 / MoS2 can be applied to the fields of solar photovoltaic cell preparation, photoelectrochemical sensor construction, photocatalysis water decomposition hydrogen manufacturing, photocatalytic organic pollutant degrading and the like.

The invention discloses a preparation method of an electrochemical biosensor. According to the method, a novel two-dimensional manometer electrode material-doped titanium dioxide / molybdenum disulfide composite material Cu-TiO2 / MoS2 is firstly prepared, and the material is a copper-doped titanium dioxide manometer square in-situ composite molybdenum disulfide two-dimensional manometer composite material; good biocompatibility and large specific surface areas of the material are utilized; a nonyl phenol antibody is loaded; during detection, copper-doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ; an electrochemical signal is generated; the influence of the specificity quantitative combination of the antibody and an antigen on the electronic transmission capability is utilized, so that the current intensity is correspondingly reduced; the building of a biosensor for detecting nonyl phenol by an unmarked electrochemical method is finally realized.

The invention relates to a preparation method of a CNTs / NiFe3O4 functional material, and belongs to the technical field of functional nano composite materials and environmental biology. The preparation method of the CNTs / NiFe3O4 functional material comprises the following specific steps: purifying and activating multiwalled carbon nanotubes; preparing CNTsFe3O4: preparing the CNTs / NiFe3O4 nano composite material. The CNTs / NiFe3O4 functional material prepared by the invention is used as an efficient biological catalytic material and a wave-absorbing material, has the characteristics of promoting oxidation-reduction reaction and electron transfer, is used as a stabilizer and a catalyst in sewage treatment anaerobic digestion reaction, can improve the methane yield and produce biomass energy,can play a role once being added, and has a good application prospect. The catalyst is good in dispersity in a reactor and uniform and consistent in reaction, and can be recycled to save the cost.

The invention provides an electromagnetic power device, which produces combined ampere forces to reduce the self-weight of the device or produces powers in other directions through circuits of asymmetric closed loops containing capacitors and AC (alternating current) or pulse DC (direct current) power sources. By using the conventional electromagnetism principle, the suspense problem for overcoming gravity is solved. The electromagnetic power device has the advantages of obvious power production effect, low cost and wide potential application.

The invention discloses a preparation method of a two-dimensional nano electrode material which is free of noble metal doping, low in cost, easy to prepare and high in electrooptic activity, and belongs to the technical field of novel nano functional materials and green energy. The method comprises the steps that a copper doped titanium dioxide nanosheet material is composited in situ on a molybdenum disulfide nanosheet of a copper intercalation layer, and the two-dimensional nano composite material Cu-TiO2 / MoS2 is obtained by in-situ compositing molybdenum disulfide with copper doped titanium dioxide nano blocks through a one-pot method. The prepared Cu-TiO2 / MoS2 can be applied to the fields of solar energy photovoltaic cell preparation, photoelectrochemistry sensor construction, photocatalytic water splitting for hydrogen production, photocatalytic degradation of organic pollutants and the like.

The invention relates to a water-soluble fluorine-containing modified N-NVP polymer and a preparation method thereof. The preparation method comprises the step: N-NVP and DFMA are subjected to a polymeric reaction to obtain the compound shown as the formula (I). According to the invention, as a fluorine-containing hydrophobic alkyl side chain is introduced to the water-soluble N-NVP polymer, the N-NVP polymer has the advantages of excellent hydrophobic association property, high surface activity, and excellent salt, temperature and shear resistant properties, can serve as a macromolecular surfactant, and meanwhile has a high utilization potentiality in the fields of oil exploitation, paints, medicines, daily chemicals and the like.

The invention discloses a preparation method of an electrochemiluminescent bisphenol A biosensor based on a nitrogen-doped titanium dioxide nanosheet and belongs to the technical field of novel nano functional materials and biosensors. A novel flaky magnetic nano light-sensitive material FeMn-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a bisphenol A antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ, and an electrochemical reaction is generated with a base solution, high and stable electrochemiluminescence signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the electrochemiluminescence signals are correspondingly reduced, and finally the electrochemiluminescent biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects bisphenol A is prepared.

The invention discloses a preparation method of an electrogenerated chemiluminescence furazolidone biosensor and belongs to the technical field of novel nano functional materials and biological sensors. A novel two-dimensional nano composite material Mn-TiO2 / g-C3N4 is prepared first, the material is loaded with furazolidone antibodies by means of good biocompatibility and large specific surface area, then by means of the cross-linking effect of glutaraldehyde, horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen enzyme in situ in detection, an electrochemical reaction is performed on a base solution, a high and stable electrogenerated chemiluminescence signal is generated, by means of influences of specific quantitative combination of antibodies and antigens on electron transmission capacity, the electrogenerated chemiluminescence signal is correspondingly reduced, finally the low-cost, high-sensitivity and good-specificity electrogenerated chemiluminescence biosensor for detecting furazolidone is prepared, and the biosensor is fast in detection and easy to prepare.

The invention discloses tubular clay mineral-magnetic metal nano composite and a preparation method thereof. Magnetic metal nano particles are located in natural nano tubes of clay mineral. The preparation method includes: performing organic modification on the tubular clay mineral to allow the nano tubes of the clay mineral to be hydrophobic; mixing the tubular clay mineral with a certain proportion of metal organics; in-situ synthesizing metal oxide nano particles in the nano tubes of the clay mineral by means of high-temperature thermal decomposition; reducing metal oxide to obtain the tubular clay mineral-magnetic metal nano composite. The novel tubular clay mineral-magnetic metal nano composite has the advantages such as stable and novel structure, uniform particle size and worthiness of application and is potentially and widely applicable in fields such as degradation-resistant waste water treatment, electromagnetic wave absorption, drug carrier and sustained release preparation, and magnetic resonance imaging. The preparation method is simple in process flow.

The invention relates to in-situ crosslinked alginate hydrogels with an active ingredient of disulfide bond disulfide bond alginate derivative. The preparation method thereof is as follows: the organic liquids of carbodiimide hydrochloride and N-hydroxysuccinimide eater are added into the alginate aqueous liquid of the alginate to activate the carboxyl of the alginate aqueous liquid; then the organic liquid of 4-aminothiolphenol is added under the conditions of keeping in dark place under 10 DEG C and nitrogen protection, so that the amino of the 4-aminothiolphenol and the carboxyl of the alginate aqueous liquid form an amido bond. The proportions of each matter adjusted and the pH value of muriatic acid is adjusted to 6.0, ethanol is deposited, frozen and dried to obtain sulfhydrylation alginates of different sulfydryl contents. The alginate aqueous liquid of the alginate oxidizes the sulfydryl thorugh the oxygen in the liquid under a room temperature to crosslink and form a disulfide bond, thus obtaining the crosslinked alginate hydrogels. The hydrogels prepared through the method has pH sensitivity and reduction responsiveness, and has potential application values in drug delivery field.

The invention discloses a method for preparing a photocatalyst free of precious metal doping, low in cost, easy to prepare and high in photocatalytic activity and belongs to the technical field of novel nanometer functional materials and green energy.The molybdenum oxide / titanium dioxide composite nanometer photocatalyst prepared from the method is carbon nitride on-situ synthesis cobalt doping molybdenum oxide / titanium dioxide nanosheet two-dimensional nanocomposite Co-MoO3 / TiO2@g-C3N4, and has good photocatalytic activity.

The invention relates to a benzene-ring-containing modified acrylamide water-soluble amphoteric polymer and a preparation method thereof. Amphoteric monomer methylacryloyloxyethyl-N,N-dimethylpropyl sulfonate (DMAPS), hydrophobic monomer methyl styrene (alpha-MST) and acrylamide (AM) are copolymerized in a lauryl sodium sulfate (SDS) emulsion by using potassium persulfate (KPS) as an initiator. Due to the introduction of the benzene ring structure comprising large steric hindrance, the water-soluble hydrophobic association polymer prepared by the method has the advantages of excellent hydrophobic associativity, excellent surface activity and excellent temperature / salt / shear resistance, can be used as a high molecular surfactant, and has wide application potential in the fields of oil-gas exploitation, paints, medicine and the like.

The invention discloses a preparing method of an electrochemiluminescence carbamate sensor based on a cobalt-doped two-dimensional nanometer composite, and belongs to the technical field of novel nanometer functional materials and biosensors. The preparing method comprises the steps that firstly, a novel two-dimensional manometer material Co-MoO3 / TiO2@g-C3N4 is prepared, and a carbamate antibody is loaded by using the good biocompatibility and the large specific area of the material; secondly, horse radish peroxidase is fixed through the cross-linking action of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ and is subjected to an electrochemical reaction with a base solution to generate high and stable electrochemiluminescence signals, then the influence on the electron transmittability by the specific and quantitative combination of the antibody and an antigen is used, the electrochemiluminescence signals are reduced correspondingly, and finally the carbamate detecting electrochemiluminescence biosensor low in cost, high in sensitivity and specificity, fast in detection and easy to prepare is obtained.

The invention discloses a preparation method of a photoelectric chemical chloramphenicol biosensor based on a two-dimension nanometer photoelectric material, and belongs to the technical field of novel nanometer function materials and biosensors. The preparation method comprises the steps of firstly preparing the novel two-dimension nanometer photoelectric material and loading a chloramphenicol antibody and fixing alkaline phosphatase through good biocompatibility and large specific surface area of the material, wherein the material is a two-dimension nanometer compound material Mn-TiO2-MoS2 which is formed by in-situ composition of manganese doped titanium dioxide diamonds and molybdenum disulfide. In detection, the alkaline phosphatase can catalyze L-ascorbic acid-2-trisodium phosphate AAP to in situ generate L-ascorbic acid AA and further can provide an electron donor for photoelectric detection, the light current intensity can be correspondingly reduced through the effect of quantitative combination of specificity of the antibody and antigen to electronic transmission capacity, and finally construction of the biosensor for detecting chloramphenicol through an mark-free photoelectric chemical method is achieved.

The invention discloses a preparation method of a photoelectrochemical furazolidone sensor, and belongs to the technical fields of novel nano functional materials and biosensors. According to the preparation method, a novel dual metal co-doped two-dimensional photosensitizer is prepared at first, namely, iron and manganese co-doped titanium dioxide nano blocks and molybdenum disulfide carry out in-situ synthesis to generate a two-dimensional nano composite material (FeMn-TiO2 / MoS2), which has a good biocompatibility and large specific area, then a furazolidone antibody is loaded on the two-dimensional nano composite material, alkaline phosphatase is fixed on the two-dimensional nano composite material; when the photosensitizer is used for detection, alkaline phosphatase can catalyze L-ascorbic acid-2-phosphate trisodium salt (AAP) to generate L-ascorbic acid (AA) in-situ; thus electron donors are provided for photoelectric detection; due to the influence of specific and quantitative combination between antibody and antigen on the electron transmission performance, the photoelectric current strength is correspondingly reduced, and finally a photoelectric sensor for detecting furazolidone through a label-free photoelectric method is manufactured.

The invention discloses a method for preparing an electrochemical transducer for detecting carbamic acid ester, and belongs to the technical field of novel nano functional materials and biosensors. The method comprises the steps that firstly, a novel two-dimensional nano electrode material, a titanium dioxide / molybdenum disulfide doped composite Co-TiO2 / MoS2, namely, a cobalt doped titanium dioxide nano square in-situ synthesis molybdenum disulfide two-dimensional nanocomposite is prepared, the good biocompatibility and large specific surface area of the material are utilized, and carbamate antibodies are loaded. When detection is carried out, due to the fact that cobalt doped titanium dioxide can catalyze hydrogen peroxide to be reduced to generate O2 in situ, electrochemical signals are generated, the specificity of the antibodies and antigens is quantitatively combined with the influence on the electronic transmission capacity, the current intensity is correspondingly reduced, and finally the biosensor for detecting carbamic acid ester through a mark-free electrochemical method is constructed.

The invention discloses a manufacturing method and application of an electrochemiluminescence nonyl phenol sensor based on a titanium dioxide nanosheet composite and belongs to the technical field of novel nano functional materials and bio-sensors. The manufacturing method includes: preparing a novel two-dimensional sheet magnetic nano photosensitive material FeCo-N@TiO2; utilizing good biocompatibility, large specific surface area and ferromagnetism to load an upper nonyl phenol antibody; detecting through horse radish peroxidase having cross-linking effect, wherein the horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ; enabling the oxygen and a base solution to be in electrochemical reaction to generate high and stable electrochemiluminescence signals, utilizing influence on electron transport capability by specific quantitative combining of the antibody and antigen to correspondingly reduce the electrochemiluminescence signals, and finally obtaining the electrochemiluminescence bio-sensor which is low in cost, high in sensitivity and specificity, quick in detection, simple to manufacture and supportive of detection on nonyl phenol.