Preparation of keratin-sodium alginate composite microporous gel and application of gel as drug carrier

A technology of sodium alginate and keratin, which is applied in the direction of drug combinations, medical preparations of non-active ingredients, non-active ingredients of polymer compounds, etc., to achieve good swelling and deswelling properties

Inactive Publication Date: 2015-09-09
NORTHWEST NORMAL UNIVERSITY
View PDF5 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium alginate can be used to prepare spherical and membranous drug carriers, however, the pH dependence of sodium alginate will show that it has certain defects when releasing the drug

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of keratin-sodium alginate composite microporous gel and application of gel as drug carrier
  • Preparation of keratin-sodium alginate composite microporous gel and application of gel as drug carrier
  • Preparation of keratin-sodium alginate composite microporous gel and application of gel as drug carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Dissolve 0.1 g of feather keratin in 2 mL of 0.8 mol / L urea solution, under the protection of inert gas, add 2 mg of dithiothreitol, stir at 30 ° C for 80 min for reduction reaction; add 0.1 g of sodium alginate After stirring for 10 minutes, add 25 mg of organic cross-linking agent N, N-methylenebisacrylamide and mix well, add 1.5 mg of initiator ammonium persulfate and stir for 0.5-3 hours, then add 0.08 g of inorganic cross-linking agent calcium chloride, and continue stirring React for 0.1 h; then let the reaction solution stand at 60°C for 6 h; soak in ethanol and water, wash and freeze-dry to obtain keratin-sodium alginate composite microporous gel. The composite microporous gel has a cumulative release rate of 82% for doxorubicin hydrochloride at body temperature (37°C).

Embodiment 2

[0046] Dissolve 1.0 g of feather keratin in 5 mL of sodium hydroxide solution with a concentration of 1.0 mol / L, under the protection of inert gas, add 10 mg of dithiothreitol, stir at 45°C for 60 min; add 0.5 mg of sodium alginate , after stirring for 15 min, add 50 mg of organic cross-linking agent N,N-methylenebisacrylamide and mix well, add 15 mg of initiator ammonium persulfate and stir for 1 h, then add 0.1 g of inorganic cross-linking agent calcium chloride, continue The reaction was stirred for 0.5 h; then the reaction solution was left standing at 85°C for 3 h; soaked in ethanol and water, washed and freeze-dried to obtain a keratin-sodium alginate composite microporous gel. The composite microporous gel has a cumulative release rate of 76% for doxorubicin hydrochloride at body temperature (37°C).

Embodiment 3

[0048] Dissolve 2.0 g of feather keratin in 10 mL of urea solution with a concentration of 2.0 mol / L, add 100 mg of mercaptoethanol under the protection of inert gas, and stir at 65°C for 40 min for reduction reaction; add 1.0 g of sodium alginate and stir for 30 min After 1 min, add 100 mg of organic cross-linking agent N,N-methylenebisacrylamide and mix well, add 50 mg of initiator ammonium persulfate and stir for 3 h, then add 0.5 g of inorganic cross-linking agent magnesium chloride, and continue stirring for 1 h; Then the reaction solution was left to stand at 70°C for 4 h; soaked in ethanol and water, washed and freeze-dried to obtain a keratin-sodium alginate composite microporous gel. The composite microporous gel has a cumulative release rate of 81% for doxorubicin hydrochloride at body temperature (37°C).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
cumulative release rateaaaaaaaaaa
Login to view more

Abstract

The invention provides preparation of a keratin-sodium alginate composite microporous gel, belonging to the fields of composite materials and biotechnology. The preparation method comprises the following step: carrying out self-crosslinking polymerization on the raw materials biocompatible natural high-polymer protein and polysaccharide (keratin and sodium alginate) under the actions of an organic crosslinking agent, an inorganic crosslinking agent an initiator. The composite microporous gel has favorable swelling and deswelling properties, is sensitive to pH value, and has slow-release effects on both low-molecular and high-molecular drugs. The in-vitro drug release performance experiment indicates that the composite microporous gel can implement controlled release of drug molecules by utilizing the acid sensitiveness, and thus, can be used as a drug carrier in drug controlled release.

Description

technical field [0001] The present invention relates to a keratin-based composite material, in particular to the preparation of a keratin-sodium alginate composite microporous gel; the present invention also relates to the use of the keratin-sodium alginate composite microporous gel as a drug carrier The application belongs to the field of composite materials and the field of biotechnology. Background technique [0002] Keratin, which is a tough structure (hair, horn, nail, shell, etc.) element of the animal body, has a rigid structure due to the disulfide bridge (-S-S-) between the protein chains. However, disulfide bridges also make the digestion and degradation of keratin quite difficult. Therefore, undegraded keratin is difficult to use as feed. At present, the vast majority of feather keratin waste is only disposed of by landfill or incineration, which will cause environmental pollution and is not in line with the concept of sustainable development. [0003] In recen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08F291/06C08F289/00C08F251/00C08F222/38C08J3/24C08J3/075A61K47/42A61K47/36A61K31/704A61P35/00
Inventor 王荣民何玉凤郭菊花吕思瑶潘素娟
Owner NORTHWEST NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap