Preparation of porous sericin sponge material

Abstract

Preparation of sponge-like porous materials of silk protein is carried out by converting its conformation with denaturant at low temperature to have frozen phase separation to obtain the product. Its parameters can be regulated by controlling concentration of the silk protein, amount of denaturant or its characteristics, freezing time or post-treatment in porosity, pore size, and mechanical performance. Neither cross-linker nor pore-generating agent nor surfactant nor organic solvent with strong toxicity is used. It is produced in a simple process, and has a uniform texture, high porosity up to 98%, good toughness and elasticity and reliability, water absorbing rate up to 5000%, elastic recovery rate 100% and press deformation >90%.

Description

technical field

[0001] The invention belongs to the fields of polymer chemistry, tissue engineering, genetic engineering, biochemistry, protein, medicine and biotechnology, and specifically relates to a method for preparing a three-dimensional porous material by using natural biological macromolecule silk fibroin. Background technique

[0002] Silk is a natural protein fiber composed of 18 amino acids. China is the hometown of silk, and the application of silk in China has a history of thousands of years. Silk is widely used as clothing material due to its unique pearl luster, good air permeability, soft texture and other properties. Silk fibroin obtained by degumming natural silk or waste silk is non-toxic to the human body, has weak antigenicity, and has good biocompatibility with the human body. Its research and application in the field of biomedicine has increasingly aroused the interest and interest of researchers at home and abroad. Pay attention to. In recent decad...

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