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Preparation of porous sericin sponge material

A silk fibroin, three-dimensional porous technology, which is applied in the field of natural biological macromolecules-silk fibroin for preparing three-dimensional porous materials, can solve the problems of uniformity, flexibility, insufficient porosity and water absorption of the preparation process, and achieves low cost and high cost. The effect of biosafety, simple process

Inactive Publication Date: 2005-02-23
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still deficiencies in the preparation process, material uniformity, flexibility, porosity, water absorption, etc.

Method used

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  • Preparation of porous sericin sponge material
  • Preparation of porous sericin sponge material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Immerse about 10 g of silkworm cocoons without chrysalis in 2L of 0.5% NaHCO 3 solution, stirred and boiled for 55min, then removed and washed with deionized water. After repeating the above operation once, dry the silk at 65°C for 24 hours and put it in a desiccator for later use.

[0029]3 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of BrLi solution with a concentration of 9.5 mol / L, and dissolved under constant temperature and low speed magnetic stirring at 45°C. The protein solution was poured into a cellulose dialysis bag, and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 2.32%, and the silk protein concentration was adjusted to 1.0%.

[0030] Take 5mL of the above silk protein solution, add 3mL of n-butanol dropwise under a constant-temperature water bath with a stirring rod at a constant speed at 25°C, stabilize it for 2 minutes, pour it into a pl...

Embodiment 2

[0031] Embodiment 2: About 30g waste silk is immersed in 3L 0.5%Na 2 CO 3 solution, stirred and boiled for 45min, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 65°C and then put into a desiccator for later use.

[0032] 6 g of degummed silk after the above treatment was weighed and dissolved in 100 mL of BrLi solution with a concentration of 10.0 mol / L, and dissolved under constant temperature and low speed magnetic stirring at 45°C. Pour the protein solution into a cellulose dialysis bag, dialyze with deionized water for three days to obtain an 8% silk protein aqueous solution, and then adjust the silk protein solution concentration to 5% by dilution.

[0033] Take 10 mL of the above silk protein solution, add 5 mL of acetone dropwise with constant stirring in a constant temperature water bath at 25°C, stabilize for 2 minutes, and put it into a low-temperature refrigerator for 24 hours at -30°C. After the sil...

Embodiment 3

[0034] Example 3: Immerse about 20 g of waste silk into 2 L of 0.5 mol / L boric acid-borax buffer solution, stir and boil for 60 min, take it out, and wash it with deionized water. After repeating the above operation once, dry the silk at 65°C and put it in a desiccator for later use.

[0035] 12 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of LiBr solution with a concentration of 10.0 mol / L, and dissolved under constant temperature and low speed magnetic stirring at 45°C. The protein solution was poured into a cellulose dialysis bag, and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 11%, and the silk protein concentration was adjusted to 10% by dilution.

[0036] Take 10mL of the above silk protein solution, add 10mL of tert-butanol dropwise in a constant temperature water bath at 25°C, stabilize it for 2 minutes, pour it into a ball and put it into a low-temperature refrig...

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Abstract

Preparation of sponge-like porous materials of silk protein is carried out by converting its conformation with denaturant at low temperature to have frozen phase separation to obtain the product. Its parameters can be regulated by controlling concentration of the silk protein, amount of denaturant or its characteristics, freezing time or post-treatment in porosity, pore size, and mechanical performance. Neither cross-linker nor pore-generating agent nor surfactant nor organic solvent with strong toxicity is used. It is produced in a simple process, and has a uniform texture, high porosity up to 98%, good toughness and elasticity and reliability, water absorbing rate up to 5000%, elastic recovery rate 100% and press deformation >90%.

Description

technical field [0001] The invention belongs to the fields of polymer chemistry, tissue engineering, genetic engineering, biochemistry, protein, medicine and biotechnology, and specifically relates to a method for preparing a three-dimensional porous material by using natural biological macromolecule silk fibroin. Background technique [0002] Silk is a natural protein fiber composed of 18 amino acids. China is the hometown of silk, and the application of silk in China has a history of thousands of years. Silk is widely used as clothing material due to its unique pearl luster, good air permeability, soft texture and other properties. Silk fibroin obtained by degumming natural silk or waste silk is non-toxic to the human body, has weak antigenicity, and has good biocompatibility with the human body. Its research and application in the field of biomedicine has increasingly aroused the interest and interest of researchers at home and abroad. Pay attention to. In recent decad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/06C08J9/28
Inventor 邵正中曹正兵陈新周平姚晋荣
Owner FUDAN UNIV
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