Preparation of porous sericin sponge material

A silk fibroin, three-dimensional porous technology is applied in the field of preparing three-dimensional porous materials from natural biological macromolecules silk fibroin, which can solve the problems of uniformity, flexibility, insufficient porosity and water absorption of the preparation process, and achieve good elasticity and casting performance. Good, high porosity effect

Inactive Publication Date: 2006-07-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still deficiencies in the preparation process, material uniformity, flexibility, porosity, water absorption, etc.

Method used

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  • Preparation of porous sericin sponge material
  • Preparation of porous sericin sponge material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: About 10g of pupa silkworm cocoons were immersed in 2L 0.5% NaHCO 3 In the solution, stir and boil for 55 minutes, then take it out, and wash it with deionized water. After repeating the above operation once, dry the silk at 65°C for 24 hours and put it in a desiccator for later use.

[0029]Weigh 3 g of the degummed silk after the above treatment, dissolve it in 100 mL of a BrLi solution with a concentration of 9.5 mol / L, and dissolve it under low-speed magnetic stirring at a constant temperature of 45°C. Pour the protein solution into a cellulose dialysis bag and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 2.32%, and adjust the silk protein concentration to 1.0%.

[0030] Take 5 mL of the above silk protein solution, add 3 mL of n-butanol dropwise under a constant-speed stirring rod in a constant temperature water bath at 25°C, pour it into a plastic box after stabilizing for 2 minutes, and then...

Embodiment 2

[0031] Example 2: Immerse about 30g of waste silk into 3L 0.5% Na 2 CO 3 In the solution, stir and boil for 45 minutes, then take it out and wash it with deionized water. After repeating the above operation twice, the silk is dried at 65°C and put into the dryer for later use.

[0032] Weigh 6 g of the degummed silk after the above treatment and dissolve it in 100 mL of a BrLi solution with a concentration of 10.0 mol / L, and dissolve under low-speed magnetic stirring at a constant temperature of 45°C. Pour the protein solution into a cellulose dialysis bag, dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 8%, and then dilute to adjust the silk protein solution concentration to 5%.

[0033] Take 10 mL of the above-mentioned silk protein solution, add 5 mL of acetone dropwise with constant stirring in a constant temperature water bath at 25°C, stabilize for 2 minutes, and freeze it in a low-temperature refrigerator at -30...

Embodiment 3

[0034] Example 3: About 20g of waste silk was immersed in 2L of 0.5mol / L boric acid-borax buffer solution, stirred and boiled for 60 minutes, then taken out, and washed with deionized water. After repeating the above operation once, the silk is dried at 65°C and placed in a desiccator for later use.

[0035] Weigh 12 g of the degummed silk after the above treatment, dissolve it in 100 mL of LiBr solution with a concentration of 10.0 mol / L, and dissolve it under low-speed magnetic stirring at a constant temperature of 45°C. Pour the protein solution into a cellulose dialysis bag and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 11%, and adjust the silk protein concentration to 10% by dilution.

[0036] Take 10 mL of the above silk protein solution, add 10 mL of tert-butanol dropwise under a constant temperature water bath at 25°C, pour it into the ball after stabilizing for 2 minutes, and put it in a low-temperature r...

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Abstract

Preparation of sponge-like porous materials of silk protein is carried out by converting its conformation with denaturant at low temperature to have frozen phase separation to obtain the product. Its parameters can be regulated by controlling concentration of the silk protein, amount of denaturant or its characteristics, freezing time or post-treatment in porosity, pore size, and mechanical performance. Neither cross-linker nor pore-generating agent nor surfactant nor organic solvent with strong toxicity is used. It is produced in a simple process, and has a uniform texture, high porosity up to 98%, good toughness and elasticity and reliability, water absorbing rate up to 5000%, elastic recovery rate 100% and press deformation >90%.

Description

Technical field [0001] The invention belongs to the fields of polymer chemistry, tissue engineering, genetic engineering, biochemistry, protein, medicine, and biotechnology, and specifically relates to a method for preparing a three-dimensional porous material from a natural biological macromolecule silk fibroin. Background technique [0002] Silk is a natural protein fiber composed of 18 kinds of amino acids. China is the hometown of silk, and silk has been used in China for thousands of years. Silk is widely used as clothing material due to its unique pearl luster, good air permeability, soft texture and other properties. The silk fibroin obtained from the degumming of natural silk or waste silk is non-toxic, weakly antigenic, and has good biocompatibility with the human body. Its research and application in the field of biomedicine has increasingly attracted the interest of domestic and foreign researchers. Pay attention. In recent decades, with the deepening of people's resea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/00C08J9/28A61L27/22C08H1/06
Inventor 邵正中曹正兵陈新周平姚晋荣
Owner FUDAN UNIV
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