Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing honeycomb silk fibroin porous microsphere sustained drug release vector

A technology of silk fibroin and porous microspheres, which is used in drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve the effects of good biocompatibility, excellent biocompatibility, and easy operation.

Active Publication Date: 2016-08-10
ZHEJIANG UNIV
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of silk fibroin to regulate the application of hydroxyapatite in the field of sustained drug release is still little research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing honeycomb silk fibroin porous microsphere sustained drug release vector
  • Method for preparing honeycomb silk fibroin porous microsphere sustained drug release vector
  • Method for preparing honeycomb silk fibroin porous microsphere sustained drug release vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In this embodiment, the preparation method of the honeycomb silk fibroin porous microsphere drug slow-release carrier includes the following steps in turn:

[0037] (1) dissolving the fibrous silk fibroin formed after silkworm cocoon degumming, and obtaining an aqueous silk fibroin protein solution after filtration, dialysis, and concentration;

[0038] (2) Adjust the concentration of the silk fibroin solution in step (1) to 0.1 mg / mL and disperse it by ultrasound, put it into a dialysis bag and soak it in different times of human simulated body fluid. : 100 rpm.

[0039] (3) The shaker treatment time is 14 days, and the simulated body fluid in step (2) is replaced every 24 hours to obtain the microsphere suspension;

[0040] (4) The microsphere suspension in step (3) was washed several times with deionized water and absolute ethanol, centrifuged, and freeze-dried for 5 hours to obtain apatite / silk fibroin microspheres with a diameter of 1-5 μm. ;

[0041] (7) The bi...

Embodiment 2

[0043] The biomineralization method of the tussah silk fibroin that promotes cell growth in the present embodiment comprises the following steps successively:

[0044] (1) dissolving the fibrous silk fibroin formed after silkworm cocoon degumming, and obtaining an aqueous silk fibroin protein solution after filtration, dialysis, and concentration;

[0045] (2) Adjust the concentration of the silk fibroin solution in step (1) to 50 mg / mL and disperse it by ultrasonic waves, put it into a dialysis bag and soak it in different times of human simulated body fluid, the solution temperature is 37°C, and the rotation speed is: 200 rpm.

[0046] (3) The shaker treatment time is 1 day, and the simulated body fluid in step (2) is replaced every 24 hours to obtain the microsphere suspension;

[0047] (4) The microsphere suspension in step (3) was washed several times with deionized water and absolute ethanol, centrifuged, and dried at 60°C for 10 hours to obtain apatite / silk fibroin mic...

Embodiment 3

[0053] The biomineralization method of the tussah silk fibroin that promotes cell growth in the present embodiment comprises the following steps successively:

[0054] (1) dissolving the fibrous silk fibroin formed after silkworm cocoon degumming, and obtaining an aqueous tussah silk fibroin protein solution after filtration, dialysis and concentration;

[0055] (2) Adjust the concentration of the tussah silk fibroin protein solution in step (1) to 2 mg / mL and disperse it ultrasonically, put it into a dialysis bag and soak it in 5 times the simulated body fluid of the human body, the solution temperature is 37 °C, and the rotation speed : 20 rpm.

[0056] (3) The shaker treatment time is 7 days, and the simulated body fluid in step (2) is replaced every 24 hours to obtain the microsphere suspension;

[0057] (4) The microsphere suspension in step (3) was washed several times with deionized water and absolute ethanol, centrifuged, and freeze-dried for 48 hours to obtain apatit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing a honeycomb silk fibroin porous microsphere sustained drug release vector. Fibrous fibroin formed after cocoon degumming is dissolved, filtered, dialyzed and concentrated to obtain silk fibroin aqueous solution; the silk fibroin aqueous solution is subject to ultrasonic dispersion, placed in a dialysis bag and placed in simulated human body fluids of different times concentrations to be soaked, the silk fibroin aqueous solution is processed in a uniform shaking table, the simulated human body fluids are replaced at time intervals to obtain microsphere suspension; and the microsphere suspension is washed many times and undergoes centrifugal separation and drying to obtain apatite / silk fibroin microsphere powder as the sustained drug release vector. Apatite microspheres of a certain size and pore diameters can be formed on the surface of silk fibroin, the dispersity, drug loading performance and sustained release performance are excellent, the honeycomb silk fibroin porous microsphere sustained drug release vector has no toxic or side effect on normal cells, the biocompatibility of the drug vector is improved remarkably, and the honeycomb silk fibroin porous microsphere sustained drug release vector has wide application prospects in the fields such as sustained drug release, tissue engineering and enzyme engineering.

Description

technical field [0001] The invention discloses a preparation method of a honeycomb silk fibroin porous microsphere drug slow-release carrier, which belongs to the field of biomedical materials. Background technique [0002] Silk fiber is one of the earliest natural proteins used by humans. The natural protein fiber mainly composed of silk fibroin is a biodegradable biopolymer material with excellent biocompatibility, mechanical properties and low immunogenicity. Ideal material for the preparation of biomaterials. In recent years, with the rapid development of silk protein materials in biomedical technology, they have been widely used in many fields such as health care products, biomedicine, tissue engineering, and drug sustained release. . [0003] The role of drug slow-release carrier is to provide continuous drug delivery to the site of administration (such as: tumor tissue), and the drug sustained release for a long time (for example, several days or weeks) provides the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/19A61K47/42A61K47/02A61K31/704A61P35/00
CPCA61K9/19A61K31/704A61K47/02A61K47/42
Inventor 杨明英帅亚俊毛传斌
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com