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Kit for processing antigen-antibody immune complex in serum or plasma sample and application of kit

An immune complex and antigen-antibody technology, which is applied in the field of sample processing kits and antigen-antibody immune complex processing kits, can solve problems such as blockage of plate washer, inapplicability to daily detection, and aging of plate washer pipelines. Widely applicable, high clinical application value, and the effect of improving detection sensitivity

Active Publication Date: 2014-03-26
朱之炜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, those skilled in the art can think that this solution will produce a large amount of thick foam when washing the plate, which will easily cause the blockage of the plate washer. In addition, the long-term use of the acidity of pH2.2 will easily cause the aging of the pipeline of the plate washer. The protocol is not suitable for clinical medium and long-term daily detection use

Method used

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  • Kit for processing antigen-antibody immune complex in serum or plasma sample and application of kit
  • Kit for processing antigen-antibody immune complex in serum or plasma sample and application of kit
  • Kit for processing antigen-antibody immune complex in serum or plasma sample and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Research and preparation of immune complex sample treatment solution

[0023] In this example, the processing methods refer to the previous processing methods of the applicant in the patent publication No. CN101832999A, that is, 100ul samples plus 50ul prepared reagents are treated at 56°C for 30min, and then each relevant detection kit is used. to test.

[0024] (1) Selection of buffer

[0025] The binding reaction between antigen and antibody is reversible. Antigen-antibody complexes can also be dissociated under a certain external environment such as low pH, high concentration of salt, and repeated freezing and thawing. Under this condition, the dissociated antigen and antibody still maintain the original structure, activity and specificity. Among them, changing pH and ionic strength is the most commonly used method to promote dissociation.

[0026] In view of the above principles, this embodiment first adjusted the pH value and ionic strength, and foun...

Embodiment 2

[0065] Example 2 Determination of the Best Treatment Mode of the Antigen-Antibody Immune Complex Treatment Kit

[0066] (1) Preparation of antigen-antibody immune complex

[0067] Referring to the literature, HAV (hepatitis A virus) immune complex was prepared to verify the dissociation effect of the antigen-antibody immune complex treatment kit prepared in Example 1 and the optimal time and temperature for dissociation.

[0068] Add a certain titer of HAV antiserum to a certain concentration of HAV antigen, detect it with a HAV antigen detection kit (self-made kit) before adding and after neutralization, and add it to the HAV antigen with a concentration of 160EU / ml For the antiserum with a titer of 20U / ml, the OD value detected by the HAV antigen kit before adding the antiserum was 0.821, and the OD value after adding the 20U / ml antiserum was 0.087.

[0069] (2) Determination of the best treatment method

[0070] The antigen-antibody immune complex processing kit was used ...

Embodiment 3

[0075] Embodiment 3 The treatment agent of the present invention can also achieve dissociation effect as an added component of the sample diluent

[0076] Through the comparative study in Example 2, it is found that the dissociation is caused by the increase of the reaction temperature or the prolongation of the reaction time, until reaching or approaching the plateau. Based on this, whether the degree of dissociation changes with the addition amount and time of the antigen-antibody immune complex treatment reagent was further verified in this example, and the reaction temperature was determined to be 37°C. The results are shown in Table 9.

[0077] Table 9 Comparative study of treatment agent addition ratio and treatment time

[0078]

[0079]The above results show that 100ul sample + 50ul treatment agent treatment at 37°C for 15 minutes is still the best treatment method (because of its short reaction time and easy operation); but at 37°C 100ul sample + 10ul treatment age...

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Abstract

The invention discloses a kit for processing an antigen-antibody immune complex in a serum or plasma sample. The kit is prepared from a meta-acid buffer solution serving as a solvent, high-concentration salt ions, an ionic surfactant, a nonionic surfactant, a reducing agent, urea, trehalose and a defoaming agent. The kit is suitable for processing the serum or plasma sample which is suspected to generate the antigen-antibody immune complex to further influence the antigen or antibody detection result, and the processed serum or plasma sample is suitable for the sample detection, such as enzyme-linked immunoassay and chemiluminescence immunoassay, while the sample which does not generate the immune complex does not affect the normal detection result after being processed via the conditioning fluid of the kit. The kit disclosed by the invention can effectively dissociate the formed antigen-antibody immune complex in the sample, greatly improves the detection sensitivity of the sample, and has a very high clinical application value.

Description

technical field [0001] The invention relates to a sample processing kit, in particular to an antigen-antibody immune complex processing kit in a serum or plasma sample and its application, belonging to the technical field of reagents for medical testing. Background technique [0002] When the body is infected with a pathogen, the antigen is one of the infectious markers that appear before the antibody. Antigen detection is used in serological examination as the most direct and earliest evidence of the existence of a pathogen. However, due to the low detection rate, Clinically, the detection of antibodies is usually used as a sign of infection. After the pathogen infects the body, there is still a period of time for the emergence of antibodies. In this window period, the detection of antibodies will be missed, but the principle of antibodies can be detected. However, when a large number of antibodies appear in the serum, they combine with the antigen to form an antigen-antib...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 朱之炜田建军欧兰香朱新兴王佳颖陈振王岩寇宗阳丁兴龙
Owner 朱之炜
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