15 results about "TREHALOSE DIHYDRATE" patented technology

The invention provides a continuous crystallization method of block-shaped trehalose crystals, which comprises the following steps: stimulating trehalose syrup to crystallize to form seed syrup, growing the crystal at 60-65 DEG C for 1-2 hours, and then cooling and crystallizing to obtain the block-shaped trehalose crystals, the cooling crystallization is three-stage cooling crystallization and comprises the following steps: cooling to 50-55 DEG C at a cooling rate of 0.5-0.8 DEG C / h, then cooling to 40-43 DEG C at a cooling rate of 1-1.5 DEG C / h, and then cooling to 25-30 DEG C at a cooling rate of 2-2.5 DEG C / h. Compared with a commercially available trehalose product, the purity of the trehalose product is greater than 99.8%, the length-diameter ratio is less than 2: 1, the grain size of 97wt% or more seed crystals is greater than 60 meshes, the filtering time is shortened by 62.2%, the bulk density of the product is improved by 49.3%, the particles are uniform, and when the trehalose product is compounded with other products, the proportioning amount can be accurately mastered, and the quality of the compounded product is improved.

This invention relates to the field of semiconductor cleaning technology and discloses a cleaning agent for semiconductor silicon wafers and its preparation method. This cleaning agent aims to solve the problem that traditional strong alkaline or phosphate-based wet electronic chemical cleaning agents easily corrode the silicon wafer surface, damage the crystal lattice, and thus affect electrical performance. This cleaning agent utilizes a multi-component synergistic effect: a surfactant system composed of alkyl glycosides and sophorolipids enhances particle wetting and stripping; lipase and silicon-pharmacoprotein precisely catalyze the removal of specific contaminants; sodium gluconate complexes metal ions; gelatin-polyacrylamide copolymer disperses contaminants to prevent secondary contamination; glycerol and trehalose maintain enzyme activity; and ultrapure water ensures the system meets the electronic-grade purity standards for wet electronic chemicals. Overall, it avoids strong alkaline corrosion, maintains the integrity of the silicon wafer lattice, and improves particle removal efficiency, meeting the high standards required for wet electronic chemical-based silicon wafer cleaning agents in advanced processes.

According to the crystalline trehalose drying device provided by the utility model, the infrared radiation light wave plate is arranged in the drying container, the hot air mechanism is arranged to convey hot air into the drying container, and the air exhaust and pressure reduction device is arranged to perform air exhaust and pressure reduction on the interior of the drying container; therefore, based on the characteristics of high energy utilization rate, high drying speed, large contact surface between hot air drying and a drying medium and good heat conduction effect of infrared radiation drying, reduced-pressure radiation drying, reduced-pressure boiling drying or cooling drying can be carried out on the crystalline trehalose in the drying container, moisture in the crystalline trehalose is fully removed, and the drying efficiency of the crystalline trehalose is improved. And the preparation efficiency of the anhydrous trehalose is obviously improved, so that the yield of the anhydrous trehalose is increased. In addition, an air pressure meter is arranged to obtain the internal air pressure of the drying container, and then the internal air pressure during drying is adjusted; and a first temperature sensor and a second temperature sensor are arranged to obtain the hot air temperature and the internal temperature of the drying container, so that the internal temperature during drying can be adjusted, and the drying efficiency is further improved.

The invention provides a mesenchymal stem cell cryopreservation solution and a preparation method thereof, and belongs to the technical field of cell engineering. According to the cryopreservation liquid, diluted DMEM is used as a basic culture medium, and trehalose, L-proline, polyethylene glycol, sodium carboxymethyl cellulose, sodium glycinate, sericin, glycerin, resveratrol and other components are introduced as additives on the basis of the basic culture medium. The formula of the cryopreservation liquid adopts a DMSO-free and serum-free scheme, sodium glycinate, sericin and glycerol are used as composite protective agents, ice crystal damage can be effectively relieved, osmotic pressure balance can be maintained, cell membrane and protein structures can be protected, the effect is better than that of DMSO and glycerol, and cytotoxicity is avoided. In addition, resveratrol, coryne, astragaloside and other components are used as growth factors, so that the activity of the mesenchymal stem cells in a cryopreservation state is maintained. The survival rate of the umbilical cord mesenchymal stem cells cryopreserved for 2 years can reach 99.83% at most, the cell viability OD value reaches 0.96, and the cell adherence and growth conditions are good when the cells are observed under a microscope.

The present application relates to the technical field of crystalline trehalose, and particularly relates to a continuous crystallizer for producing crystalline trehalose and a crystallization method thereof, which comprises the following steps: obtaining the amplitude of ultrasonic reflection wave of an ion exchange resin column; determining the feeding flow of trehalose concentrated solution based on the first conductivity value at the outlet of a liquid collecting cavity; continuously injecting the trehalose concentrated solution into the inlet of the ion exchange resin column according to the feeding flow, and obtaining the second conductivity value at the outlet of the liquid collecting cavity; re-determining the feeding flow of the trehalose concentrated solution according to the second conductivity value; obtaining the microcrystalline content at the outlet of the liquid collecting cavity; determining the crystallization temperature of the corresponding crystallization tank based on the microcrystalline content; crystallizing the desalted trehalose concentrated solution according to the crystallization temperature, and sequentially performing crystal slurry separation, mother liquor recovery and drying on the crystal slurry obtained after crystallization, so as to obtain the finished product of crystalline trehalose. The present application realizes the improvement of the crystallization quality of the finished product of crystalline trehalose.

The invention relates to the technical field of cosmetics, and particularly discloses a gel mask patch containing endogenous crystal patterns and a preparation method thereof.The gel mask patch comprises a matrix gel layer and an endogenous crystal structure, and a matrix is composed of 60-80% of water, 1-3% of gelling agent, 1-10% of polyhydric alcohol and 5-18% of active matter; the active matter is a eutectic system of trehalose / mannitol and glycine / taurine, and snowflake-shaped crystals protruding out of the surface by 0.1-0.3 mm are formed. During preparation, cold air nucleation is performed at 20-30 DEG C, then shock cooling directional growth is performed at 5-10 DEG C, and finally low-temperature drying and curing are performed at 40-45 DEG C. According to the invention, active matters beneficial to skin are added, and temperature change in a hydrogel production process is utilized, so that the active matters are condensed from a dissolved state and are separated out by self-crystallization, a snowflake-shaped pattern effect is formed, physical embossing or injection molding is not depended on, and the appearance of the product changes randomly and is rich and diversified; when the mask is applied, the branched crystals protrude out of the surface of the mask body to form physical attachment with the skin, gliding is prevented when the mask is used, and the functions of physical massage and physiological improvement on the skin are achieved.

The application provides a mesenchymal stem cell cryopreservation solution and a preparation method thereof, and belongs to the technical field of cell engineering. The cryopreservation solution takes diluted DMEM as a basic culture medium, and introduces trehalose, L-proline, polyethylene glycol, sodium carboxymethyl cellulose, sodium glycine, sericin, glycerol, resveratrol and other components as additives. The cryopreservation solution formula adopts a DMSO-free and serum-free scheme, uses sodium glycine, sericin and glycerol as a composite protective agent, can effectively alleviate ice crystal damage, maintain osmotic pressure balance and protect cell membranes and protein structures, and has an effect superior to DMSO and glycerol and no cytotoxicity. In addition, the application uses resveratrol, coenzyme and astragaloside and other components as growth factors to maintain the activity of mesenchymal stem cells in a frozen state. After umbilical cord mesenchymal stem cells are cryopreserved for 2 years according to the application, the survival rate of the umbilical cord mesenchymal stem cells can reach 99.83% at most, the cell viability OD value reaches 0.96, and the cells are observed to have good adhesion and growth conditions under a microscope. △ The survival rate of the umbilical cord mesenchymal stem cells can reach 99.83% at most, the cell viability OD value reaches 0.96, and the cells are observed to have good adhesion and growth conditions under a microscope.

The present application belongs to the field of biotechnology and cosmetics, and relates to a complex capable of stabilizing polypeptides and a preparation method thereof, comprising the following components in parts by weight: trehalose 3-15, lecithin 0.5-10, glycerol 65-90, water 1-10 and cosmetic polypeptide 0.03-1. Compared with the prior art, the lecithin adopted in the present application is easy to form a lamellar liquid crystal structure, can form a hollow spherical monolayer or multilayer liposome with a bilayer structure in an aqueous solution, can wrap cosmetic water-soluble or water-insoluble polypeptides in an internal hydrophilic layer or an oleophilic layer, avoids the reaction of exposed residues of the polypeptides with substances in the outside world, and leads to the disappearance of the activity of the polypeptides, and meanwhile, trehalose can be filled between phosphatidylcholine in a liquid crystal phase, can replace the position of water when the phosphatidylcholine is dehydrated, inhibits the phosphatidylcholine from changing from a liquid crystal phase to a gel phase, and protects the structure and function of the liposome.

The utility model provides a crystallized trehalose recrystallization device which comprises a rack and a crystallization kettle, the crystallization kettle is horizontally placed, stirring blades are arranged in the crystallization kettle, and materials are stirred by the stirring blades in the recrystallization process, so that the materials can be fully stirred, and the problem that the crystallization quality and efficiency are ordinary due to non-uniform stirring is solved. Flexible scrapers are arranged on the outer sides of the stirring blades, so that materials attached to the inner wall of the crystallization kettle can be effectively cleaned, crystallization materials are prevented from being accumulated on the inner wall of the crystallization kettle, efficient operation of equipment is further maintained, and the crystallization efficiency and the product quality are further improved. In addition, the first cooling pipe and the second cooling pipe which are spirally arranged on the outer side of the crystallization kettle realize efficient convection cooling through the water inlet and the water outlet in opposite directions, and uniform temperature distribution in the kettle is ensured, so that the crystallization effect is optimized, and the product purity is improved. And after the material crystallization is completed, the telescopic assembly drives the crystallization kettle to lift, so that the discharge port is positioned at a lower position, and the material can be discharged more easily.

The invention provides a cryopreservation composition of induced pluripotent stem cells and a preparation, cryopreservation and recovery method of the induced pluripotent stem cells, and relates to the technical field of stem cell cryopreservation. The cryopreservation composition comprises a basic buffer solution, and dimethyl sulfoxide, trehalose, reduced glutathione, Y-27632 and lipidosome loaded with an ice recrystallization inhibitor which are dissolved or dispersed in the basic buffer solution. According to the composition, intracellular targeted delivery of the ice recrystallization inhibitor is realized by using the lipidosome, and extracellular toxicity is avoided while lethal intracellular ice crystals are inhibited; a synergistic system constructed by trehalose, glutathione, Y-27632 and the like is matched, so that membrane stability, oxidative stress resistance and resuscitation apoptosis inhibition are considered. The comprehensive protection at physical and biochemical levels obviously improves the recovery rate and the pluripotent stability of the induced pluripotent stem cells, and effectively solves the problem that multiple damage mechanisms are difficult to consider in the traditional technology.

The invention belongs to the technical field of biotechnology and cell engineering, and particularly relates to a-80 DEG C one-step overspeed vitrification cell cryopreservation solution and a preparation method thereof, and the preparation method comprises the steps of cryopreservation solution preparation, cell pretreatment and mixing, and-80 DEG C one-step overspeed vitrification cryopreservation. Program cooling or ice nucleus induction is not needed, and cell cryopreservation can be efficiently carried out in a refrigerator at the temperature of-80 DEG C in a conventional laboratory. Wherein the freezing medium core is composed of trehalose, a choline type amphiphilic ionic surfactant and a polyproline anti-ice crystal peptide in a ternary synergistic proportion, and has multiple functions of permeability protection, ice crystal inhibition, membrane stabilization and the like. Compared with an existing cryopreservation solution only containing high-concentration DMSO, the cryopreservation solution has the advantages that the dosage of DMSO can be remarkably reduced, the cytotoxicity burden can be relieved, and meanwhile, the survival rate and the function recovery capability of various mammalian cells are improved.

The invention discloses an SOD (superoxide dismutase) compound preparation and a preparation process thereof, and relates to the technical field of biochemical engineering. The preparation is composed of an SOD-trehalose eutectic nucleus, a lecithin / cholesterol bilayer and a hyaluronic acid targeting shell. The composition comprises the following components in percentage by mass: 0.5 to 1.2 percent of SOD (superoxide dismutase), 8 to 12 percent of trehalose, 4.5 to 6.0 percent of lecithin / cholesterol and 0.8 to 1.5 percent of hyaluronic acid. The preparation process comprises the following steps: 1) micro-fluidic low-temperature eutectic: emulsifying SOD and trehalose at 4 + / -1 DEG C through a micro-fluidic chip, and freezing at-30 + / -2 DEG C to form eutectic particles of 2.5 + / -0.3 mu m; 2) supercritical in-situ embedding: the particles react with lecithin / cholesterol for 40 + / -5 min in supercritical CO at 8 + / -0.5 MPa / 15 + / -2 DEG C, and the encapsulation efficiency is greater than or equal to 87%; and 3) cold spray drying: adding hyaluronic acid for homogenizing, and drying under the conditions that the air inlet temperature is less than or equal to 35 DEG C and the atomization pressure is 0.15 + / -0.02 MPa. The process realizes that the SOD activity recovery rate is greater than or equal to 92%, the transdermal permeation amount is increased by 3.2 times, the storage half-life period at 4 DEG C is predicted to reach 18 months through an acceleration test, and the problems of large activity loss, low encapsulation efficiency and thermal damage in the traditional process are solved.

This invention discloses a low-temperature chelation preparation method for high-purity ferrous citrate with antioxidant properties, belonging to the field of pharmaceutical raw material preparation technology. Addressing the shortcomings of existing ferrous citrate preparation processes, such as easy oxidation of ferrous iron, severe secondary oxidation during drying, and the inability to simultaneously achieve high purity and long-term antioxidant effects, this invention employs a segmented gradient low-temperature chelation process to prepare high-purity ferrous citrate wet crystals. It deeply couples trehalose glassy embedding with a low-temperature oxygen-free closed-loop gradient drying process. In the two-step drying process of vacuum pre-freezing dehydration at -5-0℃ and oxygen-free nitrogen closed-loop drying at 15-20℃, deep dehydration of the product, in-situ formation of a monomolecular citric acid passivation film on the crystal surface, and dense formation of a trehalose amorphous glassy film are simultaneously achieved. This constructs a dual-layer synergistic protection system with an inner layer of chemical passivation and an outer layer of physical barrier. This invention introduces no exogenous antioxidants throughout the entire process, and the prepared ferrous citrate has a purity ≥99.5%, possessing high purity, strong antioxidant properties, and excellent rehydration properties, making it suitable for a wide range of applications.

This invention discloses a lyophilized formulation of fosfomycin sodium for injection and its preparation method. The lyophilized formulation comprises fosfomycin sodium, L-histidine, trehalose dihydrate, glycine, and disodium edetate, wherein L-histidine serves as a buffer component, trehalose dihydrate serves as an amorphous matrix protectant, glycine serves as a crystalline framework agent, and disodium edetate serves as a metal ion chelating agent. The preparation method includes solution preparation, pH adjustment and fine filtration, pre-freezing and annealing, primary drying, secondary drying, and nitrogen sealing. This invention, through the synergistic combination of quaternary excipients, specifically solves multiple technical problems in the fosfomycin sodium system, such as epoxy hydrolysis degradation, difficulties in lyophilization due to high sodium content, and extreme hygroscopicity. The resulting lyophilized formulation exhibits intact appearance, rapid reconstitution, and good stability.

The invention relates to the technical field of medical cold therapy equipment, and particularly discloses a sterile cold compress patch and a preparation process thereof. A gel core of the patch comprises a gel matrix, a neutralizer, an active drug, a crystallization inhibitor, a humectant and water for injection. The core of the invention is that the crystallization inhibitor is a compound prepared from polyvinylpyrrolidone K30 and trehalose through a specific hot melting composite process, and the compound can effectively inhibit crystallization and separation of active drugs in the storage and transportation process through the synergistic effect of steric hindrance and competitive hydrogen bonds. Therefore, the accuracy of the product dosage and the medication safety are guaranteed. Meanwhile, by optimizing the swelling and neutralizing process of the gel matrix, the gel network can still maintain a long-term stable three-dimensional structure and appropriate viscoelastic characteristics while bearing high-content drugs and inhibitors, and the problem that high-load gel is prone to dehydration shrinkage is solved.