9 results about "Ferrous citrate" patented technology

The invention discloses a method for producing mRNA (messenger ribonucleic acid) template plasmids by fermenting escherichia coli. When the recombinant escherichia coli is fermented and cultured, a seed solution of the recombinant escherichia coli is inoculated into a basic culture medium to be fermented and cultured until the OD600 value of fermentation liquor is 18-22, then a fed-batch culture medium is continuously added at the feeding rate of 5-15 mL / L / h to be subjected to continuous fed-batch fermentation culture, the basic culture medium contains 0.05-0.2 mmoL / L of ferric salt compound and 0.05-0.2 mmoL / L of copper chloride dihydrate, and the basic culture medium contains 0.05-0.2 mmoL / L of ferric salt compound and 0.05-0.2 mmoL / L of copper chloride dihydrate. The basal culture medium contains 10-15 g / L of copper chloride dihydrate and 100-300 mg / L of p-hydroxybenzoic acid, the carbon source of the basal culture medium contains 0.1-0.3 mmoL / L of a ferric salt compound, 0.1-0.3 mmoL / L of copper chloride dihydrate and 100-300 mg / L of p-hydroxybenzoic acid, the carbon source of the fed-batch culture medium contains 360-480 g / L of glycerol, and the ferric salt compound is one or more of ferrous citrate, ferric ammonium citrate or ferrous sulfate heptahydrate. According to the method, the yield and superhelix content of the mRNA template plasmids are improved by optimizing the culture medium formula and culture conditions, and large-scale production of the high-quality target mRNA template plasmids is facilitated.

The invention relates to the technical field of food production, and discloses a prefabricated black pepper beefsteak set meal suitable for an air fryer and a combined preparation technology thereof, and the prefabricated black pepper beefsteak set meal is composed of a pickled beefsteak bag and a black pepper seasoning bag. The beefsteak is pickled by the pickling liquid, moisture and seasoning components in the beefsteak can be effectively locked, and the beefsteak still keeps tender and juicy taste after high-temperature cooking in an air fryer in cooperation with vacuum rolling and low-temperature static pickling processes. The black pepper seasoning is matched with a compound system of ferrous glycinate and ferrous citrate, so that the steak presents a uniform and bright red color. The preparation process is simple and controllable, the raw materials are green and environment-friendly, the industrial production requirement is met, and the market application prospect is wide.

The invention provides a microbial deodorant and a production process thereof, and relates to the technical field of deodorants. The microbial deodorant is prepared from the following raw materials: 40 to 45 parts of a pyroligneous liquor-ferrous citrate compound, 7 to 10 parts of a lactobacillus plantarum microbial agent, 6 to 8 parts of a lactobacillus acidophilus microbial agent, 3 to 5 parts of a bacillus licheniformis microbial agent, 2 to 3 parts of a clostridium butyricum microbial agent and 19 to 24 parts of carrageenan nano microspheres. The pyroligneous liquor-ferrous citrate compound is prepared by the following steps: adding NaHCO3 into pyroligneous liquor to adjust the pH value to be weakly acidic to obtain pretreated pyroligneous liquor, then adding a ferrous citrate solution into the pretreated pyroligneous liquor, and reacting to obtain the pyroligneous liquor-ferrous citrate compound; the carrageenan nano-microsphere is prepared from the following raw material components: sophora flower polysaccharide, carrageenan, an emulsifier, paraffin and CaCl2. The microbial deodorant disclosed by the invention has excellent deodorization performance and relatively high stability.

In an iron (II) citrate monohydrate, identified by the formula FeC6H6O7-H2O ( Ferrous citrate monohydrate or iron citrate (II) monohydrate), obtained according to a particular production method, citric acid and elemental iron are bound in a molecular complex where the iron is agglomerated in the citrate which moves to a cyclic form and the iron is bound to the O groups of the carboxylic groups 1 and 3 through ionic bonds, forming two enantiomers, D and L (R and S), both perfectly water-soluble and highly assimilable by the human body. Formula (L) & Formula (D). With a different absorption mechanism from traditional iron molecules, said iron citrate monohydrate is usable in pharmaceutical and / or nutraceutical and / or food compositions, in particular in the treatment and prevention of iron deficiency anemias and in general of all diseases and disorders associated with iron deficiency. The effectiveness is proven by several examples.

In the ferric iron citrate monohydrate defined by the chemical formula FeC6H6O7 H2O (ferrous citrate monohydrate or ferric iron citrate monohydrate) obtained according to a specific production method, the citric acid and the elemental iron are combined in a molecular complex in which the iron is gathered in the citrate moving into a ring-like form and the iron is bound to the O groups of the carboxyls 1 and 3 through ionic bonds, forming two enantiomers D and L (R and S), both of which are completely water-soluble and highly absorbable by the human body. Formula (L) and Formula (D). With a different absorption mechanism than traditional iron molecules, said ferric iron citrate monohydrate can be used in pharmaceutical compositions and / or nutritional compositions and / or food compositions, in particular for the treatment and prevention of iron deficiency anemia and all diseases and conditions associated with iron deficiency. The effectiveness is demonstrated through several examples.

This invention discloses a low-temperature chelation preparation method for high-purity ferrous citrate with antioxidant properties, belonging to the field of pharmaceutical raw material preparation technology. Addressing the shortcomings of existing ferrous citrate preparation processes, such as easy oxidation of ferrous iron, severe secondary oxidation during drying, and the inability to simultaneously achieve high purity and long-term antioxidant effects, this invention employs a segmented gradient low-temperature chelation process to prepare high-purity ferrous citrate wet crystals. It deeply couples trehalose glassy embedding with a low-temperature oxygen-free closed-loop gradient drying process. In the two-step drying process of vacuum pre-freezing dehydration at -5-0℃ and oxygen-free nitrogen closed-loop drying at 15-20℃, deep dehydration of the product, in-situ formation of a monomolecular citric acid passivation film on the crystal surface, and dense formation of a trehalose amorphous glassy film are simultaneously achieved. This constructs a dual-layer synergistic protection system with an inner layer of chemical passivation and an outer layer of physical barrier. This invention introduces no exogenous antioxidants throughout the entire process, and the prepared ferrous citrate has a purity ≥99.5%, possessing high purity, strong antioxidant properties, and excellent rehydration properties, making it suitable for a wide range of applications.

An iron supply agent includes sodium iron citrate aqueous solution prepared by stirring and mixing citric acid, water and iron combined with sodium bicarbonate, and a chelating agent mixed with the aqueous solution of sodium iron citrate. The chelating agent is lignin sulfonic acid derived from lignin waste water.

The invention belongs to the technical field of environmental protection, and discloses a method for increasing the yield of short-chain fatty acid in sludge by activating persulfate with ferrous citrate, which comprises the following steps: naturally settling sludge, removing supernate to obtain concentrated sludge, adding ferrous citrate and persulfate into the concentrated sludge, putting into a constant-temperature shaking table, sufficiently and uniformly mixing, filtering, and drying to obtain the short-chain fatty acid in the sludge. And filling nitrogen to remove oxygen, sealing, and carrying out anaerobic fermentation in a constant-temperature shaking table to produce the short-chain fatty acid. Ferrous citrate reacts with persulfate to generate sulfate free radicals and hydroxyl free radicals, meanwhile, citric acid serves as a chelating agent and can effectively complex Fe < 2 + > to prevent ferrous ions from being oxidized too fast, the continuous generation capacity of the free radicals is enhanced, and therefore sludge extracellular polymeric substances and cellular structures are remarkably damaged, and full release of organic matter is promoted. Not only is the yield of short-chain fatty acid in the sludge fermentation liquor greatly increased, but also the anaerobic fermentation time can be effectively shortened, and the treatment effect is better than that of single sodium carbonate activation or pure ferric salt activation.

This application discloses a device and a method for culturing Geobacter that produces ultra-high conductivity bio-nanowires. The device comprises a tank, at least two liquid color sensors, at least two dissolved oxygen sensors, a liquid level sensor, and a stirring paddle. The top of the tank is provided with an air valve; an upper part of the tank is provided with a feed inlet, a lower part is provided with a culture medium outlet, and the bottom of the tank is provided with a discharge outlet. This application can recycle ferrous citrate, add substrates multiple times, cultivate Geobacter that grow ultra-high conductivity bio-nanowires in batches, and achieve the amplification and cultivation of a large number of Geobacter metallireducens in the same volume.