The invention relates to the technical field of eye dressings and in particular relates to a medical biological eye cold therapy dressing and a preparation method thereof. The medical biological eye cold therapy functional dressing is prepared from the following components: sorbitol, hyaluronic acid, pearl powder, peppermint oil, borneol, a mother chrysanthemum extracting solution, cassia seed extractive and a balsam pear extracting solution. The medical biological eye cold therapy dressing has functions of diminishing inflammation and swelling, easing pain and keeping moist of eyes for a long time, is used for improving and auxiliary treating of symptoms of dry eyes, eyestrain, eye itching, eye swelling, black eyes, lacrimation, secretion increase, asthenopia, corneal ulcer and the like caused by reasons of myopia, cataract, presbyopia, glaucoma, excessive eye and the like and has function of improving amblyopia.