63 results about "Ice nucleus" patented technology

The invention discloses an anti-freezing disease prevention film agent for trees as well as a preparation method and the application thereof. The anti-freezing disease prevention film agent consists of main ingredients and water, wherein the main ingredients comprise the following components in part by mass: 200-1,100 parts of film-forming material, 1-100 parts of 2-methyl-isothiazolin-3-ketone, 0.033-3.3 parts of 5-chlorine-2-methyl-4-isothiazolin-3-ketone, 10-80 parts of dimethyl sulfoxide and 10-80 parts of ethylene glycol; and the film-forming material is a or b, wherein a is chitosan, and b is chitosan and carboxymethyl cellulose. The anti-freezing disease prevention film agent for trees can take a better control effect on frozen damages caused by low temperature, wind, ice nucleating bacteria and the like, is suitable for frost prevention and overwinter protection of sapling stems and branches of poplars, hybrid hazelnut trees, apple trees and other trees, as well as late spring cold prevention, cytospora chrysosperma prevention and canker prevention after blossom of apricot trees, pear trees and the like in early spring, and wound surface protection after scraping of scabs.

The invention discloses a high-temperature snow making method utilizing low-temperature gas for forming ice nucleuses. According to the method, a snow making machine forming the ice nucleuses through the low-temperature gas is utilized for snow making under the high-temperature condition, and the low-temperature gas includes N2 low-temperature gas and CO2 low-temperature gas. A head of the snow making machine used in the method comprises multiple nucleon devices and multiple atomizers. Each nucleon device comprises a water inlet pipeline and a gas inlet pipeline, wherein the low-temperature gas enters from the gas inlet pipeline. According to the method, the low-temperature gas enters artificial snow making equipment from the gas inlet pipelines of the nucleon devices, a traditional manner that air of the nature is compressed and enters nucleon devices for snow making is replaced, meanwhile, optimization design is conducted on the nucleon devices and the atomizers, the snow making equipment can achieve the snow making function at the temperature of -2 DEG C, and indoor ski fields of high-temperature areas of Chinese mainland parts become possible.

The invention provides a display system of a chitosanase cell surface. A fused gene segment is obtained by fusing a gene csn and a gene inaQN, wherein the gene csn is coded with chitosanase and the gene inaQN of an N-terminal structural domain of an ice nucleating protein is responsible for transmembrane transport and has an anchoring function. The fused gene segment is expressed on the surface ofa host cell, and the display system of the chitosanase cell surface is obtained. The nucleotide sequence of the gene csn is showed as SEQID NO.1. The nucleotide sequence of the gene inaQN is showed as SEQIDNO. 2. The invention further provides a preparation method of the display system of the chitosanase cell surface. The display system of the chitosanase cell surface can directly react with thechitosan in a solution, tedious steps of protein purification are saved, and cost of producing low-molecular-weight chitosan and oligomeric chitosan is reduced greatly. The display system of the chitosanase cell surface has great application prospects in the field of low molecular weight chitosan and oligomeric chitosan preparation.

The invention relates to the technical field of biotechnology and analysis technology and concretely relates to a sequential enzyme proportional bacterial surface co-display system and a use thereof. The sequential enzyme surface co-display system comprises a gene sequence (ga) for coding a target protein glucoamylase, a gene sequence (gdh-m) of a glucose dehydrogenase mutant, a gene sequence (coh-dock) of a cohesion-dockerin protein and a gene sequence inaPb-N of an ice nucleating protein N end structural domain for transmembrane positioning and transport. The bacterial surface display system is used for starch detection. The starch detection method has high sensitivity and simple processes. The whole cell catalyst can be used in the fields of biosensing, foods, medicine and health, and biological energy sources.

The invention provides a plant antifreezing solution which comprises the following components in parts by weight: 2 to 5 parts of paraffin, 5 to 8 parts of vegetable oil, 4 to 8 parts of polybutylene, 3 to 9 parts of potassium fulvic, 5 to 9 parts of benzoic acid, 2 to 6 parts of tea polyphenol, 2 to 5 parts of citric acid and 2 to 5 parts of a cooking liquor of cassia twig, sappanwood, ginger and ampelopsis japonica. The ion content of Cu, Fe, Mn, Zn and B in the antifreezing solution is greater than or equal to 100 g/L. The plant antifreezing solution is simple in composition and convenient to use, the freeze injury damage on trees can be greatly reduced, the antifreezing effect is good, and the plant antifreezing solution activates biological enzyme, prevents the generation of ice nucleus bacteria, breaks ice forming activity of freezing protein, and increases heat. The ice point value of plant cells is reduced, the flow of cellular plasm is promoted, the cell viability is improved by 4 to 8 times, the water holding and drought resisting abilities of the plant are improved, freeze injury danger is reduced, functions of reducing and recovering are played on middle and light freeze injury plants, effects on serious damage are in vain, and the ice point value of the plant is reduced by 5 to 8 degrees.

The invention discloses an off-line detection device and measurement method for ice nuclei concentration and ice-forming activity in the mode of infiltrating nuclei, comprising: a liquid separator, a PCR plate, a PCR bracket, a water bath, a computer, a lens, and a CCD camera; Temperature control, temperature recording, picture acquisition and data processing modules; the PCR plate is placed on the PCR support, and together with the PCR support is placed in the condensate in the water bath; the water bath and CCD camera are connected to the computer; through picture collection The module controls image acquisition, image storage, and data processing. The invention realizes that the liquid holding tank can hold more liquid droplets; reduces the loss of particles to be tested; adopts the PCR board to reduce the problem of mutual influence of liquid droplets in the cooling process, increases the volume of liquid droplets that can be measured, and reduces the The difficulty of liquid, reducing the error caused by inaccurate quantification of droplet volume. The invention is easy to operate, and can simultaneously perform temperature recording, image acquisition and data processing.

The invention is concerned with the soft ice machine, includes the beverage box, the feeding opening, the discharge opening, the refrigeration system, the refrigeration system consists of the metal tubes, the evaporator is layed at the bottom of the middle box of the beverage box or the location closing to the bottom of the box, the blowing ice parts touches with the metal tube or with a small gap, the blowing ice parts moves by the power steeing. It is: the refrigerant evaporates in the metal tube in order to freezing the beverage out of the metal tube, the evaporator is not only low cost but also high heat transfer coefficient, and moreover, it is simple structure and higher guidable plot ratio.

The invention discloses a carboxylesterase D-1CarE3 cell surface display system and a whole-cell catalyst as well as a preparation method and an application of the whole-cell catalyst. A construction method of the surface display system comprises the following steps: constructing a prokaryotic expression vector pET28NCD3 by utilizing an NC end of synthetic truncated ice nucleoprotein of source pseudomonas syringae as ankyrin to be fused with target protein carboxylesterase D-1CarE3, and converting the prokaryotic expression vector into escherichia coli BL21 to construct escherichia coli cell surface display engineering bacterium BL21/pET28NCD3 of the carboxylesterase D-1CarE3. Under the effect of a strong promoter T7, through IPTG inducible expression, the display of carboxylesterase D-1CarE3 cell surface activity is successfully realized, and the whole-cell catalyst D-1CarE3 is prepared by using an ultra-low temperature freeze drying method. The whole-cell catalyst D-1CarE3 can be applied to the treatment of environmental pollution of pesticide residues.

The present invention discloses a test method of an influence mechanism of the city underlying surface aerosol to a thunder and lightning process. The method comprises the steps of utilizing the weather and cloud radars to monitor the severe convection weather, observing the particulate matter distribution characteristic by an atmosphere particulate matter particle size spectrometer and a laser radar, at the same time, utilizing a thunder and lightning positioning system to observe a thunder and lightning process during a weather process; establishing a concept model of the interaction of the aerosol-cloud condensation nuclei and the ice nucleus-thunder and lightning process by the relationship between the city and upstream and downstream area aerosol distribution and the thunder and lightning activity characteristic parameters; utilizing an improved three dimensional convective cloud catalysis electric coupling mode to carry out the numerical value simulation on the mechanism that the aerosol in a city underlying surface severe convection weather influences the thunder and lightning process. According to the present invention, by improving the three dimensional convective cloud catalysis electric coupling mode, and carrying out the numerical value simulation on the established concept model, the influence mechanism of the city underlying surface aerosol to the thunder and lightning activity is disclosed.

The invention provides a low-energy-consumption electric-heating ice prevention/removal system for the surface of a warship in extreme environments. The system comprises a ship hull substrate, an electric insulation, a heat-insulating material, a low-energy-consumption rapid-heating electric heating layer and a micro-nano anti-icing coating. According to the system, on one hand, the disadvantage of the traditional micro-nano coatings that the bonding force between ice and a base body will increase due to surface icing is thoroughly solved; on the other hand, the low-energy-consumption rapid-heating electric heating layer has energy consumption of lower than 2.0W/cm<2> in 30 seconds at a low voltage of 40V or less in low-temperature working environments (-40 DEG C to 0 DEG C), thus, the micro-nano anti-icing coating is heated to a liquid droplet ice-nucleus forming temperature and has the stability of low-temperature electric heating materials, the low-energy-consumption rapid-heating electric heating layer and the micro-nano anti-icing coating are in synergism, the viscous force of water droplets to the surface of the warship is reduced, the degree of icing of the surface of the warship is effectively reduced, and thus, the aim of efficient and low-energy-consumption ice prevention/removal of the surface of the warship is achieved.

The invention discloses an air temperature and humidity automatic control system and method for a cloud chamber. The method is characterized in that air to enter the cloud chamber is divided into two parts; one part of the air is dried to form dry air; the other part of the air is humidified to form moist air; the temperature and the humidity of the dry air and the temperature and the humidity of the moist air are monitored; and according to required and preset air temperature and humidity, the dry air and the moist air are proportionally mixed and then are pumped into the cloud chamber. The system and the method may provide air with specific humidity for cloud physics and manual influence weather research atmosphere ice nucleus or ice crystal equipment.

The invention relates to a tea tree anti-freezing agent and a preparation method thereof and belongs to the technical field of tea tree anti-freezing. The tea tree anti-freezing agent comprises the following raw materials according to weight ratio: 0.05 to 0.15 percent of paclobutrazol, 0.02 to 0.05 percent of sodium benzoate, 5.0 to 10.0 percent of mineral oil, 3.0 to 5.0 percent of ethyl alcohol and water in balancing amount. The tea tree anti-freezing agent provided by the invention has the following benefits: 1, the tea tree anti-freezing agent is nontoxic, harmless and has no pollution to environment, can be directly sprayed onto plant leaf surfaces, and is simple and convenient to use, the preparation method of the tea tree anti-freezing agent is simple, and the effective freeze-proof period can reach 7 to 10 days. 2, the tea tree anti-freezing agent can activate biological enzyme, prevent the formation of the ice nucleating bacteria, induce the production of anti-freezing factors, and can improve the resistivity of plants to frozen injury. 3, the tea tree anti-freezing agent provided by the invention can form a protecting film on the surface of a tea tree, and has the capability of protecting plants from resisting coldness.

The embodiment of the invention provides an anti-icing system for water conservancy and hydropower engineering. The anti-icing system comprises a compressed air supply portion, a splitting treatment portion and at least one anti-icing device arranged underwater; the compressed air supply portion is used for compressing outside air and delivering the compressed air to the splitting treatment portion; the splitting treatment portion is connected to the downstream of the compressed air supply portion and provided with pressure reducing and speed adjusting treatment devices corresponding to the anti-icing devices, and the pressure reducing and speed adjusting treatment devices communicate with the corresponding anti-icing devices; and each anti-icing device is provided with a bubble generatingpart, and each bubble generating part is used for generating bubbles in water through the compressed air from the corresponding pressure reducing and speed adjusting treatment device. According to the anti-icing system, the air compressed by the compressed air supply portion is supplied to the bubble generating parts through the pressure reducing and speed adjusting treatment devices, the bubblegenerating parts can spray bubble clusters in the water, then a water body is driven to generate disturbance, ice nucleuses are eliminated, and destruction of ice damage to the water conservancy and hydropower engineering is avoided.

The invention provides a trunk protection liquid which comprises the following components by weight: 2-5 parts of paraffin, 5-8 parts of vegetable oil, 4-8 parts of isobutene, 3-9 parts of BFA, 5-9 parts of acetic acid, 2-6 parts of tea polyphenol, 2-5 parts of citric acid, and 2-5 parts of cooking liquid of acacia catechu, bletilla, dried chilli and tangerine peels, wherein the content of ions Cu+Fe+Mn+Zn+B in an antifreezing agent is not less than 105 g/L. The trunk protection liquid is simple in proportion, and convenient to use, can reduce the freezing injury loss of trees greatly, is good in anti-freezing effect, activates biological enzymes, prevents the ice nucleating bacteria from generation, destroys the frozen protein ice formation activity, increases heat quantity, reduces the plant cell freezing point value, promotes the flowing of cellular plasm, improves the cell viability by 4-8 times, increases the water retaining and drought capability of the plants, reduces the freezing injury, alleviates the rehabilitation effect on medium-low-grade freezing injured plants, has no effect on the high-grade injury, and reduces the plant freezing point value by 5-8 degrees.

The invention provides an antifreezing agent, wherein the antifreezing agent comprises the following components in parts by weight: 2-5 parts of paraffin, 5-8 parts of vegetable oil, 4-8 parts of glycerinum, 3-9 parts of acetic acid, 5-9 parts of salicylic acid, 2-6 parts of tea polyphenol, 2-5 parts of glacial acetic acid, and 2-5 parts of a cooking liquor of bamboo leaves, ochre, Chinese mosla herb and pine needles. The antifreezing agent is simple in ratio and convenient to use, greatly reduces the loss caused by the freeze injury of a tree, and has an excellent antifreezing effect. The antifreezing agent activates the biological enzyme, stops the production of ice nucleating bacteria, breaks the icing activity of frozen protein, increases the heat, reduces the freezing point of a vegetable cell, promotes the flow of the cellular plasm, and improves the cell viability by 4-8 times. The water-retaining and drought-resisting abilities of the plant are improved, the freeze injury is alleviated, the antifreezing agent has an effect of alleviating and recovering the moderate and mild freeze injury plants, has no effect on the severe injury, and reduces the freezing point of the plant by 5-8 DEG C.

The invention provides a preparation for preventing crops from frost damage. The preparation comprises the following components in parts by weight: 2-5 parts of paraffin, 5-8 parts of edible oil, 4-8 parts of isobutene, 3-9 parts of fulvic acid, 5-9 parts of heteroauxin, 2-6 parts of allium sativum oil, 2-5 parts of monobasic organic acid, and 2-5 parts of a cooking liquor of szechuan lovage rhizome, fructus evodiae, dry eggplant stems and rhizoma zingiberis, wherein the ion content of Cu, Fe, Mn, Zn and B in the preparation is greater than or equal to 95 g/L. The preparation provided by the invention is simple in ratio and convenient to use, can greatly reduce frost damage to trees, is good in freeze-proof effect, can activate a biological enzyme, prevents the generation of an ice nucleating bacteria, destroys ice generation activity of frozen protein by increasing heat quantity, reduces a freezing point of a plant cell, accelerates the flow of cellular plasm, improves the activity of the cell by 4-8 times, increases the water-holding and drought-resisting capacity of plants, reduces frost damage, can alleviate and cure moderate and light frost damage to the plants, is ineffective on heavy frost damage, and reduces the freezing point value of the plants by 5-8 DEG C.

The invention discloses a serialized direct-evaporation-type high-efficient multi-temperature ice storing tank. The high-efficient multi-temperature ice storing tank does not use the ethylene glycol as the heat-transfer medium, and is capable of directly conveying the high-temperature and high-pressure organic refrigerant liquid into an evaporator for evaporating and refrigerating after throttling, absorbing the sensible heat and latent heat of the water in the tank, making ice and cooling. The serialized direct-evaporation-type high-efficient multi-temperature ice storing tank is consist of the multi-temperature ice storing tank, the evaporator, a circulation water pump, an ice nucleus generator, a water flow distributor, a splitter plate, a chilled water region, a belt-type temperature sensor, a liquid level meter, an electric proportional-integral regulating valve and the like. The serialized direct-evaporation-type high-efficient multi-temperature ice storing tank is capable of providing the chilled water with different temperatures which is used for low-temperature dehumidification and indoor cooling. The advanced technologies, such as circulation water pump, the ice nucleus generator, and the water flow distributor, are used for improving the icing rate and the icing speed, the ice storing amount is large, the cost is low and the application is extensive, various air condition ice storing tanks can be replaced by the serialized direct-evaporation-type high-efficient multi-temperature ice storing tank. The serialized direct-evaporation-type high-efficient multi-temperature ice storing tank is one of the main devices of the patent 'serialized direct-evaporation-type ice-water cooling double-cold-source air condition system' (patent number 201410359231.1).

A method for identifying a sand and dust cloud mixture comprises the following steps: measuring the particle size of a solid or liquid particle suspended in a gas medium of a corresponding cloud according to an aerosol measuring instrument, and measuring the mass concentrations of particles under different particle sizes; dividing the corresponding clouds into the following types according to the mass concentration of the particles: clear sky, non-sand dust, clouds/rain, sand dust and a sand dust cloud mixture; acquiring a laser radar parameter range and a cloud radar parameter range of a corresponding cloud in a preset area at the same time and in the same space; identifying clear sky, non-sand dust, cloud/rain, sand dust and sand dust cloud mixtures based on the particle mass concentration, the lidar parameter range and the cloud radar parameter range. According to the method, the sand-dust-cloud mixture is accurately identified by utilizing the cooperation of the standard backscattering coefficient of the laser radar, the depolarization ratio of the laser radar and the equivalent reflectivity of the cloud radar, so that the sand-dust is proved to participate in activation as an ice nucleus, and a technical support is provided for deeply researching the interaction between the cloud and the sand-dust and a sand-dust cloud rainfall mechanism.

The invention discloses a detection device and system for nucleation of an atmospheric ice nucleus. The detection device comprises a humidity control component, an image acquisition component, a temperature control component and a cooling table, wherein the cooling table comprises an upper-layer cooling table cover, a lower-layer cooling table cover and a cavity; a transparent glass sheet is arranged at the top part of the upper-layer cooling table cover; a chamber is formed between the upper-layer cooling table cover and the lower-layer cooling table cover; a cavity is arranged below the lower-layer cooling table cover, and a copper table is arranged in the cavity; a hydrophobic silicon wafer for bearing a sample is arranged at the top part of the copper table, and a vent hole is formed in the lower-layer cooling table cover so that the cavity can be communicated with the hydrophobic silicon wafer; the humidity control component is connected with the chamber through a connecting piece so as to adjust humidity; a first groove is formed in the copper table, and the temperature control component is arranged in the first groove to adjust the temperature; and the image acquisition component is arranged above the transparent glass sheet so as to acquire images of the hydrophobic silicon wafer in real time through the transparent glass sheet and the vent holes. According to the detection device and the system, the temperature and relative humidity of ice crystals formed by nucleation of atmospheric particulates as ice nuclei can be detected.

A method and system of measuring the size distribution of particles within dilute colloids, for example, through variation of the minimum ice-nucleation sizes of particles within the colloid. The system for measuring particles in fluids includes, a sample fluid inlet and an ice nuclei counter communicatively connected to the sample fluid inlet, the ice nuclei counter cooling the sample fluid and measuring particles which form crystals in the cooled fluid. The method for measuring particles in fluid includes the steps of providing a sample fluid, cooling the sample fluid, and measuring particles which form crystals in the cooled fluid.