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Method for extracting beta-glucan from Sparassis crispa

An extraction method, hydrangea technology, applied in the field of dextran extraction, can solve the problems of reagent residue, affecting the quality and safety of health care products and food, and the inability to guarantee the purity and yield of glucan extraction, so as to improve the extraction rate and increase the The effect of purity

Inactive Publication Date: 2020-02-11
FUQING CITY FIRE KIRIN EDIBLE FUNGUS TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the development of dextran in hydrangea is currently receiving more and more attention and research. At this stage, most of the industry still uses acid extraction, alkali extraction, and acid-base mixed extraction methods to extract and purify dextran in hydrangea. , but most of these methods introduce strong acid and strong alkali in actual operation, not only cannot guarantee the extraction purity and yield of dextran, but also there will be certain reagent residues, which will affect the subsequent health care products and food quality of dextran Quality and Safety

Method used

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  • Method for extracting beta-glucan from Sparassis crispa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] S1: Drying, pulverizing and grinding Hydrangea bacteria to obtain superfine powder, which needs to pass through a 80-mesh sieve;

[0026] S2: adding a wall-breaking enzyme agent to the superfine powder, the addition of the cellulase and pectinase are 1200U / g and 800U / g respectively, after ultrasonic enzymolysis, free liquid is obtained;

[0027] S3: Add the free liquid into a high-pressure microwave tank, and perform high-pressure microwave extraction. The microwave power of the high-pressure microwave extraction is 900W, the pressure is 0.8MPa, and the time is 4min. After filtering, the extract is obtained;

[0028] S4: first add amylase to the extract, and perform enzymolysis reaction for 20 minutes; then add protease, and perform enzymolysis for 10 minutes. centrifuged to obtain supernatant;

[0029] S5: Add ammonium sulfate to the supernatant. When adding ammonium sulfate, it needs to be constantly stirred and carried out in a boiling water bath. After it dissolves...

Embodiment 2

[0032] S1: Drying, pulverizing, and grinding Hydrangea bacteria to obtain superfine powder, which needs to pass through a 100-mesh sieve;

[0033] S2: Adding a wall-breaking enzyme agent to the ultrafine powder, the addition amount of the cellulase and pectinase is 1600U / g and 1000U / g respectively, after ultrasonic enzymolysis, a free liquid is obtained;

[0034] S3: Add the free liquid into a high-pressure microwave tank, and perform high-pressure microwave extraction. The microwave power of the high-pressure microwave extraction is 930W, the pressure is 0.9MPa, and the time is 5min. After filtering, the extract is obtained;

[0035] S4: first add amylase to the extract, and perform enzymolysis reaction for 30 minutes; then add protease, and perform enzymolysis for 20 minutes. centrifuged to obtain supernatant;

[0036] S5: add ammonium sulfate to the supernatant, need constant stirring when adding ammonium sulfate, and carry out in boiling water bath, after it dissolves, ad...

Embodiment 3

[0039] S1: Dry, pulverize and grind Hydrangea to obtain superfine powder, which needs to pass through a 100-mesh sieve;

[0040] S2: Adding a wall-breaking enzyme agent to the ultrafine powder, the addition amount of the cellulase and pectinase is 2000U / g and 1300U / g respectively, after ultrasonic enzymolysis, a free liquid is obtained;

[0041] S3: Add the free liquid into a high-pressure microwave tank, and perform high-pressure microwave extraction. The microwave power of the high-pressure microwave extraction is 960W, the pressure is 0.9MPa, and the time is 6min. After filtering, the extract is obtained;

[0042] S4: first add amylase to the extract, and perform enzymolysis reaction for 40 minutes; then add protease, and perform enzymolysis for 30 minutes. centrifuged to obtain supernatant;

[0043] S5: add ammonium sulfate to the supernatant, need constant stirring when adding ammonium sulfate, and carry out in boiling water bath, after it dissolves, add ethanol again, a...

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Abstract

The invention discloses a method for extracting beta-glucan from Sparassis crispa. The method realizes pretreatment of the Sparassis crispa abd effective destroy of cells through physical grinding andaddition of a wall-breaking enzyme in order to dissolves out contents, so the extraction rate of the glucan is increased; high-pressure microwave treatment makes separation of glucan and cell tissuesfurther realized in order to achieve dissociation, so finally the glucan is obtained by extraction; and starch, proteins and other polysaccharides can be effectively removed by adding an impurity removal enzyme and ammonium sulfate, so that the purity of glucan is greatly improved.

Description

technical field [0001] The invention relates to the technical field of glucan extraction, in particular to a method for extracting β-glucan from hydrangea. Background technique [0002] Hydrangea, also known as hydrangea mushroom, anti-flower fungus, dry mushroom, broccoli fungus, honeycomb fungus, etc., the Latin scientific name is Sparassiscrispa, which belongs to the order of non-folded bacteria, hydrangea family, hydrangea genus. The fruiting body is medium to large in shape, fleshy, with many branches from a thick stalk, and the ends of the branches form countless zigzag petals, which are named after the shape of a huge hydrangea. Because of its super high ability to activate immunity, it is known as "dream magic mushroom" in Japan. Common mushrooms grow in the shade, but hydrangea mushrooms need more than 10 hours of sunlight every day, and are the only "sunshine mushrooms" in the world. It is extremely popular in Europe, America and Japan, and the price is expensive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0024
Inventor 陈美英王国强何绍东
Owner FUQING CITY FIRE KIRIN EDIBLE FUNGUS TECH DEV
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