A kind of Bacillus mobilis and its application

A Bacillus, exercise technology, applied in the application, bacteria, microorganisms and other directions, to achieve the effect of promoting plant growth, increasing utilization, and promoting agricultural development

Active Publication Date: 2022-06-14
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main applications of coal gangue are: taking advantage of its large carbon content and high calorific value, it can be used as a raw material for power generation and heating; using coal gangue to prepare building materials such as bricks, concrete, and cement; using coal gangue to produce Chemical products such as molecular sieves, etc., but the utilization rate of coal gangue is still not high, and there is still a large amount of coal gangue accumulation

Method used

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  • A kind of Bacillus mobilis and its application
  • A kind of Bacillus mobilis and its application
  • A kind of Bacillus mobilis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1. Isolation and screening of functional strain GZU-Bac01

[0033] 1) Collection of samples

[0034] (1) The samples were collected from the soil near the mining area;

[0035] (2) crush and sieve the collected sample, and collect a 60-mesh sample;

[0036] (3) Store the samples in a refrigerator at 4°C for later use.

[0037] 2) Screening of functional strains

[0038] (1) Accurately weigh 1g of soil sample into a sterilized 250mL Erlenmeyer flask, and add 99mL of sterile water;

[0039] (2) Shake in a constant temperature shaker at 180r / min, 30°C for 30min;

[0040] (3) After shaking for 30 minutes, take the supernatant and dilute to 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 .

[0041] (4) Take 100uL of each concentration and spread it on three insoluble inorganic phosphorus solid media;

[0042] (5) Place the plate in a constant temperature incubator at 35°C for 3 to 4 days;

[0043] (6) Streak inoculation of bacteria with different appearance characteristics...

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Abstract

The invention discloses a mobilis bacillus and application thereof. The Bacillus mobilis is named Bacillus mobilis GZU‑Bac01 (Bacillus mobilis GZU‑Bac01), and was deposited in the China Center for Type Culture Collection on December 7, 2020, with the preservation number CCTCC NO: M 2020863. Bacillus mobilis GZU-Bac01 of the present invention has the nutrient element that can dissociate and release solidification, as: nitrogen, phosphorus, potassium, calcium etc., and dissociation effect is good, and cost is low, and environmental protection, adopts microbial bacterium (Bacillus mobilis GZU‑Bac01) method for dissociating coal gangue to prepare microbial fertilizers, which not only converts insoluble and / or insoluble nutrients in coal gangue into soluble components, but also releases the soil after it is applied to the soil The solidified nitrogen, phosphorus, potassium, calcium, etc. in the plant can be transformed into nutrients directly absorbed and utilized by plants. At the same time, GZU‑Bac01 can also effectively release insoluble phosphorus and / or insoluble phosphorus in other phosphorus-containing minerals. phosphorus.

Description

technical field [0001] The invention relates to a bacterial strain and its application, in particular to a mobilis bacillus and its application. Background technique [0002] In the past few decades, China's industry has developed rapidly, and the development speed of the coal industry has also increased significantly. While bringing huge benefits to the Chinese economy, it has also brought greater harm to society and the environment. A large amount of coal gangue is produced in the process of coal mining and washing. With the continuous development of the coal industry, coal gangue has become one of the top ten industrial solid wastes in the world today. [0003] A large amount of coal gangue accumulation not only takes up a lot of valuable land resources, but also brings harm to the coal mining area and the surrounding environment, so the resource utilization of coal gangue is extremely important. At present, the main applications of coal gangue are: taking advantage of i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C05B17/00C12R1/07
CPCC12N1/20C05G3/00C05B17/00
Inventor 谢承卫王应兰李小军董丽敏盛定红杜鑫
Owner GUIZHOU UNIV
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