Dissociating agent universally used for TT3 and TT4 in serum and preparation method thereof

A dissociating agent and serum technology, which is applied to the dissociating agent and its preparation field commonly used for TT3 and TT4 in serum, can solve the problems of wrong clinical judgment, difficulty in the research and development of quantitative detection reagents for thyroid function, etc., and achieves easy control. , convenient for clinical staff, simple process effect

Active Publication Date: 2021-02-12
GUANGZHOU KEFEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Triiodothyronine (TT3) and thyroxine (TT4) are important components in the detection of clinical thyroid function items. At present, the detection reagents of the above two components commonly used in clinical practice mainly rely on the purchase of imported detection reagents. The main function of the reagent is to dissociate TT3 and TT4 in the bound state from their respective operating proteins, so as to become free molecules, so as to facilitate the detection of them by related reagents, so as to improve the accuracy of clinical measurement values ​​and avoid abnorm

Method used

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  • Dissociating agent universally used for TT3 and TT4 in serum and preparation method thereof
  • Dissociating agent universally used for TT3 and TT4 in serum and preparation method thereof

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[0027]Example 1

[0028]A method for preparing a dissociating agent commonly used for TT3 and TT4 in serum includes the following preparation steps:

[0029]Add 0.05% EDTA, 0.05% 5-bromo-2-methylpyridine, 0.05% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 0.5% 8-phenylamino to 1L deionized water After mixing the ammonium salt of -1-naphthalenesulfonate and 0.5% sodium citrate, store it at 2℃-8℃ in the dark to obtain a dissociating agent for TT3 and TT4 in serum.

Example Embodiment

[0030]Example 2

[0031]A method for preparing a dissociating agent commonly used for TT3 and TT4 in serum includes the following preparation steps:

[0032]Add 0.05% EDTA, 0.25% 5-bromo-2-methylpyridine, 0.25% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 1.0% 8-phenylamino in 1L deionized water Mix -1-naphthalenesulfonic acid ammonium salt and 1.0% sodium citrate, and store at 2℃-8℃ in the dark to obtain a dissociating agent commonly used for TT3 and TT4 in serum.

Example Embodiment

[0033]Example 3

[0034]A method for preparing a dissociating agent commonly used for TT3 and TT4 in serum includes the following preparation steps:

[0035]Add 0.05% EDTA, 0.5% 5-bromo-2-methylpyridine, 0.5% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 2.0% 8-phenylamino to 1L deionized water Mix -1-naphthalenesulfonic acid ammonium salt and 2.0% sodium citrate, and store at 2℃-8℃ in the dark to obtain a dissociating agent commonly used for TT3 and TT4 in serum.

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Abstract

The invention discloses a dissociating agent universally used for TT3 and TT4 in serum. The dissociating agent comprises the following components in percentage by mass: 0.01%-0.1% of metal chelate; 0.01%-1.0% of an N heterocyclic compound; 0.05%-0.2% of a stabilizing agent; 0.2%-1.0% of a surfactant; and 0.5%-4.0% of an auxiliary agent and the balance of water. The dissociation agent disclosed bythe invention has a good dissociation effect, can be used as a sample pretreatment solution in an A-work project detection reagent, can well dissociate bound TT3 and TT4 in serum from respective transport proteins to enable the TT3 and TT4 to be in a free state, and then detects and verifies the content of the TT3 and TT4 in the free state by adopting a chemiluminiscence immunoassay method, and therefore, the measured value of the sample result is more accurate. The invention also provides a preparation method of the dissociating agent universally used for TT3 and TT4 in serum.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a dissociating agent commonly used for TT3 and TT4 in serum and a preparation method thereof. Background technique [0002] Triiodothyronine (TT3) and thyroxine (TT4) are important components in the detection of clinical thyroid function items. The detection reagents of the above two components commonly used in clinical practice mainly rely on the purchase of imported detection reagents. The main function of the reagent is to dissociate TT3 and TT4 in the bound state from their respective operating proteins, so as to become free molecules, so as to facilitate the detection of them by related reagents, so as to improve the accuracy of clinical measurement values, avoid abnormal results, and cause Incorrect clinical judgment. [0003] In human blood, 99.7% of triiodothyronine (TT3) is combined with transkinin, mainly with thyroxine-binding globulin (TBG) and a...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N33/78
CPCG01N1/28G01N33/78
Inventor 周剑辉王敏仪
Owner GUANGZHOU KEFEN BIOTECH CO LTD
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