Dissociating agent universally used for TT3 and TT4 in serum and preparation method thereof

Abstract

The invention discloses a dissociating agent universally used for TT3 and TT4 in serum. The dissociating agent comprises the following components in percentage by mass: 0.01%-0.1% of metal chelate; 0.01%-1.0% of an N heterocyclic compound; 0.05%-0.2% of a stabilizing agent; 0.2%-1.0% of a surfactant; and 0.5%-4.0% of an auxiliary agent and the balance of water. The dissociation agent disclosed bythe invention has a good dissociation effect, can be used as a sample pretreatment solution in an A-work project detection reagent, can well dissociate bound TT3 and TT4 in serum from respective transport proteins to enable the TT3 and TT4 to be in a free state, and then detects and verifies the content of the TT3 and TT4 in the free state by adopting a chemiluminiscence immunoassay method, and therefore, the measured value of the sample result is more accurate. The invention also provides a preparation method of the dissociating agent universally used for TT3 and TT4 in serum.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a dissociating agent commonly used for TT3 and TT4 in serum and a preparation method thereof. Background technique [0002] Triiodothyronine (TT3) and thyroxine (TT4) are important components in the detection of clinical thyroid function items. The detection reagents of the above two components commonly used in clinical practice mainly rely on the purchase of imported detection reagents. The main function of the reagent is to dissociate TT3 and TT4 in the bound state from their respective operating proteins, so as to become free molecules, so as to facilitate the detection of them by related reagents, so as to improve the accuracy of clinical measurement values, avoid abnormal results, and cause Incorrect clinical judgment. [0003] In human blood, 99.7% of triiodothyronine (TT3) is combined with transkinin, mainly with thyroxine-binding globulin (TBG) and a...

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Problems solved by technology

[0002] Triiodothyronine (TT3) and thyroxine (TT4) are important components in the detection of clinical thyroid function items. At present, the detection reagents of the above two components commonly used in clinical practice mainly rely on the purchase of imported detection reagents. The main function of the reagent is to dissociate TT3 and TT4 in the bound state from their respective operating proteins, so as to become free molecules, so as to facilitate the detection of them by related reagents, so as to improve the accuracy of clinical measurement values ​​and avoid abnormal results, which may lead to error in clinical judgment

[0003] In human blood, 99.7% of triiodothyronine (TT3) is combined with transkinin, mainly with thyroxine-binding globulin (TBG) and a small amount of albumin, as well as thyroid-binding prealbumin (TBPA); 99.95 % of thyroxine (TT4) is reversibly bound to the transport protein, mainly thyroxine-binding globulin (TBG) and a small amount of albumin, and thyroid-binding prealbumin (TBG), this specific binding is The research and development of methazine quantitative detection reagents has brought difficulties and challenges

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