Composition extracted from animal organs
A technology of mixture and extraction method, which is applied in the direction of drug combination, medical preparations containing active ingredients, blood diseases, etc., can solve the problems of artificial synthesis, etc., and achieve the effects of short production cycle, simple operation and improved enzymatic hydrolysis process.
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Embodiment 1
[0068] A method for extracting heparin sodium, characterized in that it comprises the steps of:
[0069] (1) Add quartz sand to the mucous membrane of the small intestine of the pig, and ultrafinely pulverize it;
[0070] (2) Hydrolyze the pulverized material with enzymes, the enzyme preparation used for hydrolysis is: a mixture of cellulase: amylase: lipase in the ratio of (1):(1):(1) in parts by mass;
[0071] (3) Ion exchange fiber adsorption;
[0072] (4) Ion exchange fiber elution;
[0073] (5) Heparin sodium precipitation;
[0074] (6) Purification of heparin sodium;
[0075] (7) Dry to obtain pure heparin sodium.
Embodiment 2
[0077] A method for extracting heparin sodium, characterized in that it comprises the steps of:
[0078] (1) Add quartz sand to mucous membrane of bovine small intestine, and ultrafinely pulverize it;
[0079] (2) Hydrolyze the pulverized material with enzymes, the enzyme preparation used for hydrolysis is: a mixture of cellulase: amylase: lipase in the ratio of (1):(1):(1) in parts by mass;
[0080] (3) Ion exchange fiber adsorption;
[0081] (4) Ion exchange fiber elution;
[0082] (5) Heparin sodium precipitation;
[0083] (6) Purification of heparin sodium;
[0084] (7) Dry to obtain pure heparin sodium.
Embodiment 3
[0086] A method for extracting heparin sodium, comprising the following steps:
[0087] (1) Add 10 parts of quartz sand to 100 parts of porcine small intestine mucous membrane, ultrafinely pulverize,
[0088] (2) Hydrolyze the pulverized mixture with enzymes. The enzyme preparation used for hydrolysis is: cellulase produced by Trichoderma reesei: alkaline β-amylase: rabbit pancreatic lipase, in the form of (1): (1): (1) A mixture formed by mixing parts by mass ratio;
[0089] Add 100 parts of pulverized mixture into the reaction kettle, raise the temperature to 40°C, keep it warm for 10 minutes, add 50 parts of water, add 1 part of NaCl, add MgCl 2 0.01 part, control the temperature at 30°C, adjust the pH to 8 with lye, add 1 part of enzyme preparation, stir for 1 hour and add a precipitant, the precipitant is selected from 2 parts of polyaluminum chloride, stand for 2 hours, and remove the supernatant Filter with a 100-mesh filter cloth to collect the filtrate;
[0090] (...
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