A dissociating agent commonly used in serum for tt3 and tt4 and its preparation method

A dissociating agent and serum technology, which is applied to the dissociating agent and its preparation field commonly used for TT3 and TT4 in serum, can solve the problems of wrong clinical judgment, difficulty in the research and development of quantitative detection reagents for thyroid function, etc., and achieves easy control. , convenient for clinical staff, simple process effect

Active Publication Date: 2021-04-09
GUANGZHOU KEFEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Triiodothyronine (TT3) and thyroxine (TT4) are important components in the detection of clinical thyroid function items. At present, the detection reagents of the above two components commonly used in clinical practice mainly rely on the purchase of imported detection reagents. The main function of the reagent is to dissociate TT3 and TT4 in the bound state from their respective operating proteins, so as to become free molecules, so as to facilitate the detection of them by related reagents, so as to improve the accuracy of clinical measurement values ​​and avoid abnormal results, which may lead to error in clinical judgment
[0003] In human blood, 99.7% of triiodothyronine (TT3) is combined with transkinin, mainly with thyroxine-binding globulin (TBG) and a small amount of albumin, as well as thyroid-binding prealbumin (TBPA); 99.95 % of thyroxine (TT4) is reversibly bound to the transport protein, mainly thyroxine-binding globulin (TBG) and a small amount of albumin, and thyroid-binding prealbumin (TBG), this specific binding is The research and development of methazine quantitative detection reagents has brought difficulties and challenges

Method used

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  • A dissociating agent commonly used in serum for tt3 and tt4 and its preparation method
  • A dissociating agent commonly used in serum for tt3 and tt4 and its preparation method

Examples

Experimental program
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Embodiment 1

[0028] A preparation method for a dissociative agent commonly used in serum for TT3 and TT4, comprising the following preparation steps:

[0029] Add 0.05% EDTA, 0.05% 5-bromo-2-methylpyridine, 0.05% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 0.5% 8-phenylamino in 1L of deionized water - 1-Naphthalenesulfonate ammonium salt, 0.5% sodium citrate, mix well, store at 2°C-8°C in the dark, and obtain a general dissociating agent for TT3 and TT4 in serum.

Embodiment 2

[0031] A preparation method for a dissociative agent commonly used in serum for TT3 and TT4, comprising the following preparation steps:

[0032] Add 0.05% EDTA, 0.25% 5-bromo-2-methylpyridine, 0.25% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 1.0% 8-phenylamino in 1L of deionized water - Mix 1-ammonium naphthalenesulfonate and 1.0% sodium citrate, store at 2°C-8°C in the dark, and obtain a dissociating agent for TT3 and TT4 in serum.

Embodiment 3

[0034] A preparation method for a dissociative agent commonly used in serum for TT3 and TT4, comprising the following preparation steps:

[0035] Add 0.05% EDTA, 0.5% 5-bromo-2-methylpyridine, 0.5% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 2.0% 8-phenylamino in 1L deionized water - Mix 1-naphthalenesulfonate ammonium salt and 2.0% sodium citrate, store in the dark at 2°C-8°C, and obtain a general dissociating agent for TT3 and TT4 in serum.

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Abstract

The invention discloses a dissociating agent commonly used for TT3 and TT4 in serum, which comprises the following components in mass fraction: metal chelate 0.01%-0.1%; N-heterocyclic compound 0.01%-1.0%; stabilizer 0.05% %~0.2%; Surfactant 0.2%~1.0%; Auxiliary 0.5%~4.0%, the rest is water. The dissociation agent of the present invention has a good dissociation effect, can be used as a sample pretreatment solution in the detection reagents of methionine items, and can well dissociate TT3 and TT4 in serum from their respective operating proteins. After dissociating and making it into a free state, the content of TT3 and TT4 in the free state is detected and verified by chemiluminescence immunoassay, so that the measured value of the sample result is more accurate. The invention also provides a preparation method of a dissociative agent commonly used in serum for TT3 and TT4.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a dissociating agent commonly used for TT3 and TT4 in serum and a preparation method thereof. Background technique [0002] Triiodothyronine (TT3) and thyroxine (TT4) are important components in the detection of clinical thyroid function items. The detection reagents of the above two components commonly used in clinical practice mainly rely on the purchase of imported detection reagents. The main function of the reagent is to dissociate TT3 and TT4 in the bound state from their respective operating proteins, so as to become free molecules, so as to facilitate the detection of them by related reagents, so as to improve the accuracy of clinical measurement values, avoid abnormal results, and cause Incorrect clinical judgment. [0003] In human blood, 99.7% of triiodothyronine (TT3) is combined with transkinin, mainly with thyroxine-binding globulin (TBG) and a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N33/78
CPCG01N1/28G01N33/78
Inventor 周剑辉王敏仪
Owner GUANGZHOU KEFEN BIOTECH CO LTD
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