A dissociating agent commonly used in serum for tt3 and tt4 and its preparation method
A dissociating agent and serum technology, which is applied to the dissociating agent and its preparation field commonly used for TT3 and TT4 in serum, can solve the problems of wrong clinical judgment, difficulty in the research and development of quantitative detection reagents for thyroid function, etc., and achieves easy control. , convenient for clinical staff, simple process effect
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Embodiment 1
[0028] A preparation method for a dissociative agent commonly used in serum for TT3 and TT4, comprising the following preparation steps:
[0029] Add 0.05% EDTA, 0.05% 5-bromo-2-methylpyridine, 0.05% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 0.5% 8-phenylamino in 1L of deionized water - 1-Naphthalenesulfonate ammonium salt, 0.5% sodium citrate, mix well, store at 2°C-8°C in the dark, and obtain a general dissociating agent for TT3 and TT4 in serum.
Embodiment 2
[0031] A preparation method for a dissociative agent commonly used in serum for TT3 and TT4, comprising the following preparation steps:
[0032] Add 0.05% EDTA, 0.25% 5-bromo-2-methylpyridine, 0.25% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 1.0% 8-phenylamino in 1L of deionized water - Mix 1-ammonium naphthalenesulfonate and 1.0% sodium citrate, store at 2°C-8°C in the dark, and obtain a dissociating agent for TT3 and TT4 in serum.
Embodiment 3
[0034] A preparation method for a dissociative agent commonly used in serum for TT3 and TT4, comprising the following preparation steps:
[0035] Add 0.05% EDTA, 0.5% 5-bromo-2-methylpyridine, 0.5% 3-bromo-5-hydroxypyridine, 0.1% Proclin 300, 0.5% Tween-40, 2.0% 8-phenylamino in 1L deionized water - Mix 1-naphthalenesulfonate ammonium salt and 2.0% sodium citrate, store in the dark at 2°C-8°C, and obtain a general dissociating agent for TT3 and TT4 in serum.
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