119results about How to "Meet clinical requirements" patented technology

The present invention discloses a dentition model generation method based on oral cavity scanning data and CBCT (Cone Beam Computed Tomography) data. The method comprises: reading a three-dimensional multi-source data model of an oral cavity; setting and registering a fixed model and a floating model, wherein the fixed model is a triangular mesh model created by means of CBCT, and the floating model is a triangular mesh model of intraoral scanning; manually picking up feature point pairs of the models on the fixed model and the floating model, correcting the feature point pairs according to point cloud curvature of the models, moving feature points to a point with the largest curvature in a surrounding neighborhood, and performing preliminary registration on the feature point pairs by using a point-to-point ICP (Iterative Closest Point) algorithm; calculating a point cloud error of the models after preliminary registration, and by combination with medical parameter requirements, setting a parameter of precise registration; performing precise registration by using an optimized point-to-surface ICP algorithm; and fusing the CBCT data and intraoral scanning data after registration, and outputting tooth root and crown models after registration and fusion. Rapid and precise registration and fusion of data of different devices or data of different postures of patients are implemented.

The invention discloses a segmentation method and system for an abdomen soft tissue nuclear magnetism image. The segmentation method comprises the steps that pre-segmentation is conducted on an area to be segmented through an area growing algorithm, then a morphological operator is adopted to conduct expansion and corrosion operations to carry out further processing on the pre-segmentation result, so that the pre-segmentation result forms an original segmentation outline. After rectification is conducted between a shape template set and the original segmentation outline, kernel principal component analysis is conducted, and prior shape information is obtained through a statistics model. The prior shape information is combined with data items of an energy function of a nuclear magnetism image segmentation model, and an energy function is built; a kernel graph cuts algorithm is used for carrying out segmentation on the original segmentation outline and an objective outline is obtained. The segmentation method and system can achieve semi-automatic segmentation, the system is simple, the robustness of the nuclear magnetism image segmentation algorithm is effectively improved so as to enable the segmentation result to be more accurate, and the segmentation method and system for the abdomen soft tissue nuclear magnetism image can be applied to nuclear magnetism image segmentation.

The invention particularly relates to a preparation method of traditional Chinese medicine fine powder, and belongs to the field of traditional Chinese medicines. The preparation method particularly comprises the following steps: (1) first a traditional Chinese medicinal material is conventionally crushed that the traditional Chinese medicinal material can pass a sieve of 80-100 mesh; (2) the crushed traditional Chinese medicinal material is processed according to a conventional method; and (3) low-temperature ultramicro-pulverization of the crushed traditional Chinese medicinal material is carried out to obtain the traditional Chinese medicine fine powder with the particle diameter of less than or equal to 0.5 [mu]m and with the content of more than 98%. Through use of the preparation method, cellular level pulverization of the traditional Chinese medicinal material can be realized, the traditional Chinese medicinal material can be more thoroughly processed, and the preparation method is conducive to rapid dissolution and digestion and absorption of effective components.

The invention discloses an automatic identification and statistics method for white blood cells in a gynecologic microscopic image. A color image is firstly converted into a gray image, and an image segmentation method is used to obtain a template for the white blood cells; AND-operation is carried out on the template and the gray image; each small area is segmented; and by using the roundness of the white blood cells, the length-width ratio of an external rectangle, the area and the ratio of a cell nucleus to the cell, identification on the white blood cells is realized. Automatic identification and statistics are realized, clinical requirements are met, artificial detection and cell identification by using cell staining are replaced, and the manpower and the material resources are saved greatly.

The invention discloses a ventricular fibrillation comprehensive detection method which is based on the complexity. It mainly uses complexity calculation, adopts a number of processing tools, comprehensively detects the ventricular fibrillation combining a number of characteristic numbers, separates various kinds of electrocardiosignal types more effectively, and makes it more suitable for reflecting ventricular fibrillation signals through updating the calculation method of complexity. The sensibility and the specificity are higher and the algorithm amount of calculation is small; it fully meets clinical requirements, resolves the problems of prior patient monitor, implantable defibrillator (ICD), automatic external defibrillator (AED) and other medical equipment, such as sensibility and the specificity are low, anti-interference power is weak and other issues.

The invention relates to a gas atomization toxicant exposure box which is characterized by comprising a transparent box body, wherein the inner space of the box body is divided into a plurality of compartments by a spacing plate; a gas dispersion plate is arranged above the compartments, and breather holes are overspread on the gas dispersion plate; a box cover is arranged at the top of the box body; a gas inlet and a gas outlet are respectively arranged in the center position and the corner position of the box cover; the gas inlet is connected with an ultrasonic atomizer arranged outside the box body; a carbon dioxide sensor and an oxygen sensor are arranged in the box cover; the output ends of the carbon dioxide sensor and the oxygen sensor are connected with a signal amplification system; the output end of the signal amplification system is connected with a signal collector; the output end of the signal collector is connected with a computer; and a gas concentration monitoring module is prearranged in the computer. The invention has artful structural design and low cost, the prepared related animal models are scientific and reasonable and closer to the changes in human diseases, and the invention can be widely used in the preparation processes of various animal models.

The invention belongs to the medicine field, and particularly relats to a preparation method of finest traditional Chinese medicine powder. The technical problem solved by the invention is providing the preparation method of finest traditional Chinese medicine powder, belonging to an improved processing method of traditional Chinese medicines, and the preparation method of finest traditional Chinese medicine powder can crush medicines to a cell stage and process medicines more drastically so as to be good for effective ingredients fast dissolving, digesting and absorbing. The preparation method comprises the following steps: step A of coarsely crushing cleanly-prepared traditional Chinese medicine powder to pass through 80-100 mesh sieve at first; step B of processing according to a routine method (such as processing with wine, processing with vinegar, processing with sweet, steaming, stir-frying, processing with medicine juice and so on); further crushing the processed traditional Chinese medicine powder to the powder with powder size of 400-1200 meshes so as to be good for clinical application. Medicines are processed by the method, so that traditional Chinese medicines are furthest processed evenly and drastically; active ingredients are furthest dissolved out on an outer surface of a cell body, flavor and channel tropism of traditional Chinese medicines meet a clinical demand, and effectively matters are absorbed more fast, so that curative effects are played well.

The invention provides a wearable sleep monitor and a monitoring method. In one embodiment, the wearable sleep monitoring method comprises the following steps: step a): collecting blood oxygen saturations of a testee in preparation for sleep and during sleep, and acquiring a blood oxygen saturation parameter value; step b) in the sleeping process, when the blood oxygen saturation parameter value is reduced and the reduction maximum value is greater than or equal to a first threshold value, determining that small awakening occurs, and when the reduction duration time (D1) is greater than or equal to 10 seconds, determining that sleep apnea / hypopnea occurs once; step c), collecting electroencephalogram, snore and myoelectricity of the testee during sleep, and monitoring whether the testee has at least one of small awakening, snore and muscle activity or not during reduction of the blood oxygen saturation parameter value, and step d), repeating the steps a) to c).

The invention discloses a docetaxel nano polymer micelle freeze-drying preparation, including a methoxypolyethylene glycol-poly lactic acid segmented copolymer carrier material and docetaxel. Docetaxel is encapsulated in the carrier material, and the docetaxel and the carrier material are in the ratio of 0.01-0.15. The methoxypolyethylene glycol-poly lactic acid segmented copolymer is formed by ring open polymerization of D,L-lactide and methoxypolyethylene glycol, wherein the weight ratio of methoxypolyethylene glycol and D,L-lactide) is 1: 0.55-0.65 or 1: 0.73-0.89 or 1:0.91-0.99. Through optimization of the weight ratio of polyester and polyether in the methoxypolyethylene glycol polylactide segmented copolymer, and further optimization of the weight ratio of docetaxel and the carrier, the time of the prepared docetaxel micelle with entrapment rate more than 90% after water redissolution can reach more than 12 h, which is meets the actual situation of clinical drug application, so as to meet the clinical requirements.

A circumcision anastomat comprises an upper anastomat, a lower anastomat and a rivet fastening and adjusting part, wherein the upper anastomat is provided with an outer cutter, an inner cutter anti-slip groove, an anti-slip bump, a frenum protecting part and a plurality of rivet holes with the same size; the lower anastomat is provided with an inner cutter, an outer cutter anti-slip groove, an anti-slip bump, a frenum protecting part, a plurality of rivet holes with the same size and a plurality of rivet fastening columns with the same size; each rivet of the rivet fastening and adjusting component comprises a rivet bump, a rivet screw rod part, a rivet cap and a rivet nut; and the rivets pass through the rivet holes of the upper anastomat, the rivet holes of the lower anastomat, the fastening columns of the lower anastomat and fastening ring devices of the lower anastomat, so that the upper anastomat and the lower anastomat are connected with each other integrally. The circumcision anastomat is simple in structure and convenient to use; surgical positioning is accurate, surgical time is short, postoperation healing time is also short, infection and side effects are avoided, edema can be prevented, and the pain of a patient is reduced; compared with the traditional surgery, the surgery carried out by using the disposable circumcision anastomat has a plurality of advantages; and the circumcision anastomat is applicable to all-age male patients suffering from preputium penis diseases.

The invention relates to a preparation method of a porous hydroxyapatite and strontium oxide composite bone repair material, and belongs to the technical field of biomedical material preparation. The method comprises the steps that 88%-99% by mass of hydroxyapatite and 12%-1% by mass of strontium oxide ceramic powder are weighed and ball-milled, and mixed powder is obtained; 40%-70% by mass of the mixed powder and 60%-30% by mass of ammonium bicarbonate pore-forming agent powder are mixed to be uniform and mechanically pressed into a block pressed blank; the pressed blank is put into a graphite mold, then the graphite mold is put into a discharging plasma sintering furnace for sintering, the sintered mold is naturally cooled to room temperature along with the furnace, demolding is conducted, and the porous hydroxyapatite and strontium oxide composite bone repair material is obtained. According to the porous hydroxyapatite and strontium oxide composite bone repair material prepared through the preparation method, any binding agent and templating agent do not need to be added, the components are pure, harmful impurities do not exist, the porosity is controllable between 20% and 50%, the degradation rate is appropriate, good osteogenic induction capacity is achieved, the technology is simple and easy to carry out, and the porous hydroxyapatite and strontium oxide composite bone repair material can be used for a bone defect repair material for hard tissue regeneration or reconstruction of organisms.

A Ti / HA composition is prepared from HA (48-78 wt.%), Ti (18-48 wt.%) and water glass as passivating agent (4-5 wt.%) through mixing HA powder with Ti powder while adding water glass, baking, loadingin mould, putting in plasma discharge sintering furnace, vacuumizing, pressurizing to 20-30 MPa, heating to 800-950 deg.C at speed of 80-150 deg.C / min, and holding the temp for 2-10 min. Its advantages are high strnegth and compactness, and having bioactivity.

The invention relates to a camellia oil liposome preparation used for treating snoring and xerostomia, which belongs to the field of oil water emulsified mixture of natural vegetation. The liposome preparation is made by the base oil of functional camellia oil of the natural vegetation which is nutritious and has good wetting and moist effect on body, the mixture of aromatic pennyroyal of natural vegetation and the auxiliary seasoning oil of teal oil and medical de-ionized water. The soybean lecithin of natural vegetation oil is taken as emulsifier. The liposome emulsion of ultra-micro elaioleucite is formed by punchy homogeneous emulsification. The preparation is used for oral cavity and has good wetting quality and moisturizing ability. In addition, the preparation can deeply moisten the muscle of pharyngeal portion and die sticking and improve the blood stream of veins of the pharyngeal portion. During the function duration of eight hours, the preparation can keep the oral cavity wet, and promote breath, thus reducing or eliminating the snoring and the xerostomia. Clinical results show that the preparation can effectively improve all the indexes related to sleep test and has no untoward or adverse effects. So the camellia oil liposome preparation used for treating the snoring and the xerostomia is safe and reliable and can meet clinical requirements.

The invention relates to a method for preparing a porous hydroxyapatite / calcium pyrophosphate compound bone repair material, and belongs to the technical field of biomedical material preparation. The method includes the steps that 85%-95% by mass of hydroxyapatite and 5%-15% by mass of calcium pyrophosphate ceramic powder are weighed and are subjected to ball milling to obtain mixed powder; then 45%-55% by mass of mixed powder and 45%-55% by mass of ammonium-bicarbonate pore forming agent powder are mixed to be even, and are pressed into a block pressed blank through a machine; the pressed blank is put into a graphite mold to be placed into a spark plasma sintering furnace to be sintered, the temperature is naturally cooled to the room temperature along with the spark plasma sintering furnace, demolding is carried out, and the porous hydroxyapatite / calcium pyrophosphate compound bone repair material is obtained. According to the method for preparing the porous hydroxyapatite / calcium pyrophosphate compound bone repair material, binding agents and template agents are avoided, ingredients are pure and free of detrimental impurities, the porosity is controllable between 10% and 30%, and the degradation rate is better matched with bone tissue growth; the good ossifying inducing capability is achieved, the technology is simple and easy to operate, and the porous hydroxyapatite / calcium pyrophosphate compound bone repair material can be used as a bone defect repair material for body hard tissue regeneration or reconstruction.

A biologically absorbable composition used as internal fixer of bone fracture is prepared from polylactic acid, polyalcohol acid, tricalcium phosphate, apatite and carbon fibres through die pressing or extruding in the powder state or under existance of organic solvent. Its advantages are high biocompatibility and bone conductivity, and proper bending strength and shear strength.

The invention relates to a preparation method of a collagen diaphragm material. The method comprises the following steps: firstly, cleaning animal skin in an alkalescent solution, carrying out unhairing and cleaning treatment to the animal skin in a water solution containing Na2S and Ca(OH)2, then adjusting the pH value to be 6 to 8 by using an acidulous ammonium salt, after obtaining a collagen fiber layer, and drying after degreasing the collagen fiber layer repeatedly by adding acetone, so as to obtain a raw material; crushing, purifying, refining and freeze-drying the raw material so as to obtain the collagen diaphragm material. The collagen diaphragm material is pliable and tough in texture, excellent in stickiness, low in salinity content, and good in bioactivity and biosecurity, has a complete 3D structure, and is very suitable for clinical application.

The invention belongs to the technology field of biological medical high polymer materials, and concretely relates to a process for preparing soft lining material of fluorosilicone rubber false toot. The concrete steps comprise charging modified silicone oil which is made from acrylic mixing monomer to nonmonomerd hydroxyl silicone oil, and charging silane cross linking agent, ethylene glycol dimethacrylate, and white carbon black, by which modified silicone oil with a certain degree of polymerization and cross linking is made, charging the fluorosilicone rubber on the sealing end of the hydroxyl group, by which the fluorosilicone rubber is made. The silicon sulfide rubber with the room temperature is made by the mixing and reaction of the two kinds of rubber. The invention has the advantages of reasonable preparing line, easily available raw materials, relatively low cost, simple synthetic technology and has significant values of popularizing and using.

The invention relates to a medical nano fiber enhanced composite material. The composite material comprises a base material and nano short fiber, wherein the nano short fiber keeps a crystal form andis uniformly distributed in the base material, and the nano short fiber accounts for 1-50 mass percent of the composite material. The composite material has the advantage that a single-component polymer material has high break elongation and high flexibility, has little damage to blood vessels and other tissues, has high tensile strength and high break elongation in human tissues, and greatly improves the tensile strength of the material. The medical nano fiber enhanced composite material has high biocompatibility and biodegradability, and can meet clinical demands of medical materials.

The invention discloses an improved fat stem cell for epidermis damage repair. The fat stem cell is subjected to separation and in-vitro culture to construct the slow virus vector containing p63 gene. The recombinant vector containing p63 gene is pLV.EX3d.P / neo-EF1A>TP63 / flag>IRES / eGFP. When the p63 gene is transformed to the fat stem cell by the slow virus, the Q-PCR (quantitative polymerase chain reaction) detection indicates that the expression does not exceed the untransfected fat stem cell, and the differentiation capacity of the improved fat stem cell to the epidermis cell does not exceed the untransfected fat stem cell, thereby achieving the effect of quickly and effectively covering and repairing the wound surface. The improved fat stem cell for epidermis damage repair has the characteristic of high transformation efficiency of the target gene, can conveniently and quickly implement the long-term stable expression of the target gene, has the advantages of accessible material, high growth speed, no rejection of the autologous tissue and the like, and is widely used in the fields of burns, orthopedics and the like.

The invention relates to a biological porous bone cement prepared by compositing Sr-doped calcium-phosphorus bioglass and alpha-tricalcium phosphate and a preparation method thereof. The bone cement provided by the invention is characterized in that the porousness of the bone cement is 39-57%, and the size of a micropore is between 0.5 um and 4 um; and the compression strength is 23.6-38.5 Mpa. In the invention, through compositing the Sr-doped calcium-phosphorus bioglass and alpha-tricalcium phosphate, the porous-structured bone cement is formed. In the invention, the solidification time and microstructure of the bone cement can be adjusted through the rapid dissolving of the Sr-doped calcium-phosphorus glass so as to form a porous structure with a high porousness, and the degradation property of a hydrated product (hydroxyapatite) can be improved; and in addition, by virtue of Sr ions introduced by biodegradable calcium-phosphorus glass, the compression strength of the bone cement is improved, thereby achieving the properties such as high strength and good degradability of the bone cement better.

Disclosed are a preparation method for hepatic receptor imaging agent 99mTc-galactosyl-human serum albumin fusion interferon and the application of the albumin fusion interferon, belonging to the nuclear medicine technical field. The invention adopts a stannous reduction method for the radioactive technetium tagging of the hepatic receptor imaging agent 99mTc-galactosyl-human serum albumin fusion interferon GHSA-IFN; upon polyamide-66 thin layer chromatography analysis, the radiochemical purity of the prepared 99mTc-GHSA-IFN is more than 90% and can be stabilized for more than 6 hours at room temperature, in line with the requirements of scientific research and clinical use. The distribution in mice and the imaging results of rats show that the 99mTc-GHSA-IFN can be incepted by liver in a specific way, and the liver imaging is very clear within 10-30 minutes which is the best time for liver imaging. Therefore, the 99mTc-GHSA-IFN is a good new liver receptor function SPECT imaging agent and can be used to evaluate the liver receptor functions, diagnose liver diseases and evaluate the liver disease treatment drug efficacy; at the same time, the 99mTc-GHSA-IFN is also used to prepare liver targeted therapy drugs and the treatment drugs for viral hepatitis.

The invention discloses a spinning solution and a method for preparing human serum albumin nanofibers or films by using the spinning solution. The method comprises the following steps of: mixing human serum albumin, trifluoroethanol, ammonium hydroxide or hydrochloric acid, and beta-mercatoethanol in a ratio to obtain the spinning solution; forming by using an electric spinner or a film pressing machine; and curing and drying to obtain the nanofibers, films and other solid objects respectively. After the human serum albumin materials are soaked in hot water and ethanol, and dried, the human serum albumin materials have high-strength physical and mechanical properties and are suitable for replacing the conventional clinical products, such as medical degradable suture lines, orthopaedic materials, intracavity and in-vitro hemostatic gauze, antiadhesive membranes and other medical auxiliary materials.

The invention relates to an automatic error correction electrocardiogram system and method. The automatic error correction electrocardiogram system comprises electrode lead assemblies, a position information acquiring device, position information processing module and a signal processing module, wherein each electrode lead assembly comprises an electrode and a lead wire and is further provided with identifications of positions; the position information acquiring device is used for acquiring the position information of the identifications of the positions; the position information processing module comprises a feature receiving unit, a feature recognizing unit, a reference system unit, an information comparing and correcting unit and a result outputting unit, and is used for receiving the position information and further processing the position information; and the signal processing module is used for receiving the position information corrected by the position information processing module, adjusting the sequence, converting acquired electrocardio signals into digital signals, and finally outputting the digital signals to a display module. In the method, the automatic error correction electrocardiogram system is used for acquiring an electrocardiogram. The various electrode lead assemblies are positioned, waveform sequences are corrected automatically, therefore, misdiagnosis caused by mistaken placement positions of the electrode lead assemblies is avoided, and meanwhile, the workload of doctors is relieved.

The invention relates to a mineralized collagen based skull repairing device and a manufacturing method thereof. The mineralized collagen based skull repairing device is made of a mineralized collagen / PCL (polycaprolactone) composite material; and the appearance of the repairing device comprises a spherical segment cap at the upper part and a cylindrical plug at the lower part. A separation mold and a secondary pouring technology are adopted during preparation of the mineralized collagen based skull repairing device, and the product defect caused by air residue at the blind end of the mold is overcome. The skull repairing device made of the mineralized collagen / PCL composite material has excellent osteogenic activity, the material is gradually and passively degraded under the creeping substitution action of cells, and growth of new bones can be guided at the skull defect part. A degradation product of the composite material is neutral, acidic materials causing aseptic inflammation cannot be generated, and new bone formation isn't affected; and through composition of PCL, the skull repairing device has excellent elasticity and toughness besides enough crush resistance and bending strength, and the repairing requirement of a skull of a human body is met.

The invention discloses a full-degradable metal-based closure clamp. The full-degradable metal-based closure clamp is applicable to closing blood vessels or tubular tissues in an endoscope operation.The full-degradable metal-based closure clamp has the following advantages that the closure clamp is made of degradable pure magnesium or magnesium alloy; the closure clamp is of a V-shaped structure,and an upper arm and a lower arm of the closure clamp are connected through an arc tail for removing stress concentration; the head of the upper arm and the head of the lower arm of the closure clampare provided with closing-locking structures which achieve the closing effect; the head and shoulder of the upper arm and the head and shoulder of the lower arm of the closure clamp are provided withclamping structures which are matched with clamp-application pincers; clamping teeth which can be cooperatively closed are arranged on a clamping surface of the closure clamp body. When the clamp-application pincers clamp and close the closure clamp, the upper arm elastic structure of the closure clamp is in contact with the lower arm structure to form closure, and the matched clamping teeth on the clamping surface are closely attached to blood vessels / tissues to make the lumens closed. Due to the closure mode of the closure clamp for the blood vessels / tissues and the degradability and mechanical properties endowed by magnesium-based materials, the closing capability is high, the clamp does not easily shed, body fluid is completely degraded, and clinic requirements can be well met.

The invention discloses a bovine coronavirus VPN (virtal private network) gene, encoded recombinant protein and an application thereof. A BCoV (bovine coronavirus) indirect ELISA (enzyme linked immunosorbent assay) method is established by using the recombinant protein. The bovine coronavirus VPN has the advantages of having a good antigenic epitope and high expression in prokaryotic cells, constructing a prokaryotic expression vector, successfully establishing the BCoV indirect ELISA diagnostic method by coating the antigen with the expressed recombinant protein, determining the best conditions for the method to provide a quick and easy serological differential diagnosis method for immunological detection of bovine antibodies and epidemiological investigation, and solving current drawbacks of a diagnosis method of yak coronavirus diarrhea, which is time-consuming, laborious and cannot be diagnosed quickly, and expensive expenses of biological reagents and instruments.

The invention discloses an ultrasound breast tumor grading method based on multi-feature extraction and Linear SVM. The method comprises steps that to-be-diagnosed breast ultrasound RF data is inputted; the RF data is converted into an ultrasound B-mode image to obtain the initial suspicious lump area location; the initial suspicious lump area is segmented to obtain a suspicious lump to determinethe boundary of the suspicious lump; a feature value of the segmented suspicious lump is calculated; the calculated feature value is inputted into a classifier, and the suspicious lump area is analyzed. The method is advantaged in that based on the BI-RADS grading standard, the actually acquired breast ultrasound RF data is hierarchically detected, breast tumor ultrasound 3, 4 and 5-level classification is achieved through utilizing the multi-feature extraction method and the linear support vector machine classifier, improvement of accuracy of the doctor's diagnosis is facilitated, the algorithm time and complexity can meet clinical requirements, and the method has a certain practical value.

The invention discloses a method and device for abdomen soft tissue nuclear magnetism image segmentation. The method comprises the steps that an original outline is initialed near an objective outline; a morphological operator is used for carrying out expansion and corrosion operations on the original outline, and a band-shaped closed area is formed in and outside the objective outline to be segmented; KPCA training is conducted on a collected shape template and prior shape information is obtained through a statistics model; the prior shape information is combined with data items of an energy function of a nuclear magnetism image segmentation model, and an energy function is constructed; a kernel Graph cuts algorithm is used for carry out segmentation on the band-shaped closed area and an objective outline is obtained. The method and device for abdomen soft tissue nuclear magnetism image segmentation can achieve semi-automatic segmentation, the device is simple, the robustness of the nuclear magnetism image segmentation algorithm is effectively improved so as to enable the segmentation result to be more accurate, and the method and device for abdomen soft tissue nuclear magnetism image segmentation can be applied to most nuclear magnetism image segmentation.

The invention provides a kit and method for genotyping detection of polymorphism sites of metabolism ability genes of folic acid. The kit includes a mutation-type reaction system and a wild-type reaction system, wherein the reaction systems include rs1801133 groups, rs1801131 groups and rs1801394 groups, and the structures of primers, probes and inhibitors in each group are disclosed. The problemsof high requirements for template quality and insufficient timeliness can be solved during detection of polymorphism typing of the metabolism genes MTHFR and MTRR of folic acid, only the two tubes ofreaction systems are needed, one-time sampling and one-time closed detection can be realized, and polymorphism of three sites of 677C>T, 1298A>C and 66A>G of the gene MTHFR are detected in real time;and the kit and method have stable systems and good sensitivity and specificity.