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Preparation method of collagen diaphragm material

A collagen film and collagen technology, applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of low biological activity and biological safety, achieve high strength, low salt content, and flexible texture Effect

Active Publication Date: 2014-10-15
CHANGZHOU INST OF MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a flexible texture, good adhesion, low salt content, complete 3D structure, biological activity and biological safety for the current problems of low biological activity and biological safety of collagen membrane materials. Preparation method of duhao collagen membrane material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of raw materials

[0037] Wash the cowhide and put it into the drum, add 1% Na which is more than twice the weight of the animal hide 2 CO 3 Stir for 2 hours, wash with water, and then place the cowhide in a place containing 1% Na 2 S and 5% Ca(OH) 2 In the aqueous solution, the cowhide is dehaired, and then 5% NH 4 Cl adjusts the pH value of the solution between 6-8, washes the cowhide, and then uses a slicing machine to remove the lower layer of flesh and upper epidermis on the cowhide to obtain a collagen fiber layer, and add acetone to the collagen fiber layer for multiple degreasing Afterwards, air-dried to obtain the raw material;

[0038] (2) Fragmentation of collagen fibers

[0039] After the collagen fiber layer raw material obtained in step (1) is physically broken, it is soaked in 0.2mol / L volatile acid solution hydrochloric acid to remove salt, and the mixture of collagen fiber layer and hydrochloric acid is placed in a disperser Disper...

Embodiment 2

[0047] Example 2 is used to illustrate the preparation method of the collagen membrane provided by the present invention.

[0048] The collagen product is prepared by the same method as in Example 1, the difference is that step (1) is carried out according to the following steps: the pigskin is washed and put into the drum, and 0.1% Na of more than twice the weight of the pigskin is added. 2 CO 3 Stir for 2 hours, and wash with clean water, then place the pigskin in 10% Na 2 S and 10% Ca(OH) 2 In the aqueous solution, the pigskin is dehaired, and then 1% NH 4 The pH value of the Cl adjustment solution is between 6-8, the pigskin is cleaned, and then the lower layer of meat and the upper epidermis on the pigskin are removed using a skin slicer to obtain a collagen fiber layer, and the collagen fiber layer is added with acetone for multiple After degreasing for the first time, it is air-dried to obtain raw materials.

Embodiment 3

[0050] Example 3 is used to illustrate the preparation method of the collagen membrane provided by the present invention.

[0051] Adopt the same method as Example 1 to prepare collagen products, the difference is that step (1) is carried out according to the following steps: put into the drum after the cowhide is washed, add 5% Na which is more than twice the quality of cowhide 2 CO 3 Stir for 2 hours, wash with water, and then place the cowhide in a place containing 1% Na 2 S and 1% Ca(OH) 2 In the aqueous solution, the leather is dehaired, and then 10% NH 4 Cl adjusts the pH value of the solution between 6-8, washes the cowhide, and then uses a slicing machine to remove the lower layer of flesh and upper epidermis on the cowhide to obtain a collagen fiber layer, and add acetone to the collagen fiber layer for multiple degreasing Afterwards, it is air-dried to obtain the raw material.

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PUM

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Abstract

The invention relates to a preparation method of a collagen diaphragm material. The method comprises the following steps: firstly, cleaning animal skin in an alkalescent solution, carrying out unhairing and cleaning treatment to the animal skin in a water solution containing Na2S and Ca(OH)2, then adjusting the pH value to be 6 to 8 by using an acidulous ammonium salt, after obtaining a collagen fiber layer, and drying after degreasing the collagen fiber layer repeatedly by adding acetone, so as to obtain a raw material; crushing, purifying, refining and freeze-drying the raw material so as to obtain the collagen diaphragm material. The collagen diaphragm material is pliable and tough in texture, excellent in stickiness, low in salinity content, and good in bioactivity and biosecurity, has a complete 3D structure, and is very suitable for clinical application.

Description

technical field [0001] The invention relates to the technical field of collagen purification methods, in particular to a method for preparing a collagen membrane material from animal skin. Background technique [0002] Collagen is the most abundant and widely distributed protein in animals. It is the main structural protein of the body and the main component of tissue and connective tissue. Its unique triple-helical amino acid chain structure endows it with many useful properties: such as high tensile strength, biodegradability, low antigenic activity, low irritation and cytotoxicity, and cell growth-promoting properties. All these features make it an ideal biomedical material. [0003] Collagen has a wide range of clinical uses, and its proven functions include: promoting wound healing and growth of granulation tissue, hemostasis, filling, and biological scaffolding. Among them, the hemostatic effect of collagen is very prominent. At the same time, it also has a special...

Claims

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Application Information

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IPC IPC(8): C08J5/18C07K1/34
Inventor 钱陈杨双双高坚杰
Owner CHANGZHOU INST OF MATERIA MEDICA
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