Preparation method of collagen diaphragm material

A collagen film and collagen technology, applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of low biological activity and biological safety, achieve high strength, low salt content, and flexible texture Effect

Active Publication Date: 2014-10-15
CHANGZHOU INST OF MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a flexible texture, good adhesion, low salt content, complete 3D structure, biological activity and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1

[0036] (1) Preparation of raw materials

[0037] Wash the cowhide and put it into a drum, and add 1% Na with more than double the weight of animal skin 2 CO 3 Stir for 2h and wash with clean water, then put the cowhide in 1 to 5 times the quality of the cowhide with 1% Na 2 S and 5% Ca(OH) 2 In the aqueous solution, dehairing the cowhide, and then use 5% NH 4 Cl adjust the pH of the solution to between 6-8, wash the cowhide, and then use a skinning machine to remove the lower layer of meat and the upper layer of skin on the cowhide to obtain a collagen fiber layer, and add acetone to the collagen fiber layer for multiple degreasing Then, air-dry to obtain raw materials;

[0038] (2) Fracture of collagen fibers

[0039] After the collagen fiber layer raw material obtained in step (1) is physically broken, it is added to 0.2mol / L volatile acid solution hydrochloric acid to soak to remove salt, and the mixture of collagen fiber layer and hydrochloric acid is placed in ...

Example Embodiment

[0046] Example 2

[0047] Example 2 is used to illustrate the preparation method of the collagen membrane provided by the present invention.

[0048] The collagen product was prepared by the same method as in Example 1, except that step (1) was carried out according to the following steps: the pig skin was washed and put into the drum, and 0.1% Na with more than double the weight of the pig skin was added. 2 CO 3 Stir for 2h and wash with clean water, then put the pigskin in 1 to 5 times the weight of pigskin containing 10% Na 2 S and 10%Ca(OH) 2 In the aqueous solution, the pig skin is dehaired, and then 1% NH is used 4 Cl adjust the pH of the solution to between 6-8, wash the pig skin, and then use a skin peeling machine to remove the lower and upper epidermis on the pig skin to obtain a collagen fiber layer. The collagen fiber layer is added with acetone for more After the second degreasing, air-dry to obtain raw materials.

Example Embodiment

[0049] Example 3

[0050] Example 3 is used to illustrate the preparation method of the collagen membrane provided by the present invention.

[0051] The collagen product was prepared by the same method as in Example 1, except that step (1) was carried out according to the following steps: Wash the cowhide and put it into a drum, and add 5% Na with more than double the quality of the cowhide. 2 CO 3 Stir for 2h and wash with clean water, then put the cowhide in 1 to 5 times the quality of the cowhide with 1% Na 2 S and 1%Ca(OH) 2 In the aqueous solution, dehairing the cowhide, and then use 10% NH 4 Cl adjust the pH of the solution to between 6-8, wash the cowhide, and then use a skinning machine to remove the lower layer of meat and the upper layer of skin on the cowhide to obtain a collagen fiber layer, and add acetone to the collagen fiber layer for multiple degreasing Then, air-dried to obtain raw materials.

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PUM

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Abstract

The invention relates to a preparation method of a collagen diaphragm material. The method comprises the following steps: firstly, cleaning animal skin in an alkalescent solution, carrying out unhairing and cleaning treatment to the animal skin in a water solution containing Na2S and Ca(OH)2, then adjusting the pH value to be 6 to 8 by using an acidulous ammonium salt, after obtaining a collagen fiber layer, and drying after degreasing the collagen fiber layer repeatedly by adding acetone, so as to obtain a raw material; crushing, purifying, refining and freeze-drying the raw material so as to obtain the collagen diaphragm material. The collagen diaphragm material is pliable and tough in texture, excellent in stickiness, low in salinity content, and good in bioactivity and biosecurity, has a complete 3D structure, and is very suitable for clinical application.

Description

technical field [0001] The invention relates to the technical field of collagen purification methods, in particular to a method for preparing a collagen membrane material from animal skin. Background technique [0002] Collagen is the most abundant and widely distributed protein in animals. It is the main structural protein of the body and the main component of tissue and connective tissue. Its unique triple-helical amino acid chain structure endows it with many useful properties: such as high tensile strength, biodegradability, low antigenic activity, low irritation and cytotoxicity, and cell growth-promoting properties. All these features make it an ideal biomedical material. [0003] Collagen has a wide range of clinical uses, and its proven functions include: promoting wound healing and growth of granulation tissue, hemostasis, filling, and biological scaffolding. Among them, the hemostatic effect of collagen is very prominent. At the same time, it also has a special...

Claims

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Application Information

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IPC IPC(8): C08J5/18C07K1/34
Inventor 钱陈杨双双高坚杰
Owner CHANGZHOU INST OF MATERIA MEDICA
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