Mesenchymal stem cell cryopreservation agent

A cryopreservation agent and stem cell technology, applied in the field of bone marrow mesenchymal stem cell cryopreservation agent and its preparation, can solve the problems of cytotoxicity, stem cell damage, etc., and achieve simple and feasible operation, reasonable price, and improved survival rate of resuscitation Effect
CN107232182AInactive Publication Date: 2017-10-10陈印平
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
陈印平
Publication Date
2017-10-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a mesenchymal stem cell cryopreservation agent which comprises 0.2%w / v cane sugar, 0.5%w / v low-density lipoprotein, 3%w / v trehalose, 2%w / v lecithin, 100ppm w / v polypeptide, 1.5%w / v bFGF, 1%w / v vitamin E, 5% v / v glycerin and the balance of a DMEM (Dulbecco's Modified Eagle Medium) and fetal calf serum in a ratio of 1:1 till 100ml. In the mesenchymal stem cell cryopreservation agent disclosed by the invention, the glycerin, the trehalose, the vitamin E and the polypeptide have definite functions of preventing external damage to cells, the polypeptide also has a function of preventing cells from damage of ice crystal generated in cryopreservation, and the other components are essential for maintaining survival of specific MSCs (Mesenchymal Stem Cells). The cell cryopreservation agent disclosed by the invention is applied to cryopreservation and culture of cells, is particularly capable of increasing the survival rate of revived cryopreservation cells, and has relatively good application prospects.
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Description

technical field

[0001] The invention relates to the technical field of tissue cell culture, in particular to a cryopreservation agent for bone marrow mesenchymal stem cells and its preparation method and application. Background technique

[0002] In addition to hematopoietic stem cells that can differentiate and develop into various blood cells, bone marrow also contains mesenchymal stem cells (MSCs) that produce non-hematopoietic tissues. Because it is easier to adhere to the wall and form fibroblast-like clones, some literatures MSCs are also called adherent cells (fibroblast colony forming units (CFU-F). Because they come from the support structure of bone marrow and can serve as a trophoblast to support the growth of hematopoietic stem cells, some people also call them bone marrow stromal cells.

[0003] The multilineage differentiation potential of MSCs has been verified by in vivo and in vitro experiments. Friedenstem first confirmed that MSG has the ability to differ...

Claims

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