The invention provides a cell cryopreservation agent of hematopoietic stem cells. The cell cryopreservation agent comprises 20 mg of glutamine, 300 [mu]g of epidermal growth factors, 0.2% (w/v) of sucrose, 0.5% (w/v) of low density lipoproteins, 5% (w/v) of trehalose, 0.5% (w/v) of lecithin, 100 ppm (w/v) of polypeptides, 0.6% (w/v) of bFGF, 0.5% (w/v) of vitamin E and 2% (v/v) of glycerol, and a DMEM medium and fetal bovine serum are added to the cell cryopreservation agent according to a ratio of the DMEM medium to the fetal bovine serum of 1:1 until the volume is 100 ml. The glycerol, trehalose, vitamin E and polypeptides in the cryopreserved cells can clearly resist cell injuries brought by outside damages, the polypeptides also protect the cells from being damaged by ice crystals during freezing, and several other components are essential for maintaining the survival of specific hematopoietic cells. The cell cryopreservation agent is used for the cryopreservation of cultured cells, especially improves the survival rate of cryopreserved cells after resuscitation, and has a good application prospect.