Clinical NK cell cryopreservation liquid and cryopreservation method

A technology of NK cells and cryopreservation method, which is applied in the field of clinical NK cell cryopreservation solution and cryopreservation, which can solve the problems of inability to meet the needs of mass production, limited sources of human autologous serum, and increased introduction of animal-derived components. Achieve the effect of maintaining the activity and killing ability of frozen cells, improving the activity and killing ability of frozen cells, and protecting mechanical damage
CN112400863APending Publication Date: 2021-02-26COBAXER BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
COBAXER BIOTECH
Publication Date
2021-02-26

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Abstract

The invention discloses a clinical NK cell cryopreservation solution and a cryopreservation method. The clinical NK cell cryopreservation solution comprises the following components in percentage by volume: 3%-10% of dimethyl sulfoxide, 2%-4% of glycerol, 13%-20% of dextran, 0.1%-0.2% of polyvinylpyrrolidone, 5%-10% of N-acetylcysteine and 60%-70% of a glucose solution. Wherein the glucose solution is formed by compounding medical-grade glucose and medical-grade pure water; the mass concentration of glucose in the glucose solution is 5wt%; the clinical NK cell cryopreservation solution can bedirectly used for intravenous injection, the DMSO content is low, the toxicity to human bodies or cryopreserved cells is greatly reduced, and the clinical NK cell cryopreservation solution is safer; the cell viability and killing capability can be better preserved, and the damage to the cells in the cryopreservation process is reduced.
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Description

Technical field

[0001] The present invention relates to the technical field of cell cryopreservation, in particular to a clinically used NK cell cryopreservation solution and a freezing storage method.Background technique

[0002] Natural killer cell (Natural killer cell, NK) is a type of lymphocyte, derived from CD34+Hematopoietic progenitor cells achieve key immune regulation functions by regulating dendritic cells (DCs). As immune cells, NK cells have the function of resisting virus invasion and tumor killing. They are the body's first line of defense against tumors and have good application prospects in adoptive immunotherapy for malignant tumors. However, it occupies a very small comparison in peripheral blood, accounting for 5% to 15% of lymphocytes, and the function of NK cells in tumor patients is lacking in varying degrees, which makes it difficult to produce good anti-tumor effects. Therefore, NK cells have become one of the common types of immune cell therapy. At this stage, NK...

Claims

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