The invention belongs to the technical field of feed additives and relates to a jewfish feed phagostimulant and a preparation method thereof. The weight of the phagostimulant in each ton of jewfish feed is 13.3-25.5 kg, and the phagostimulant in each ton of jewfish feed consists of the following raw materials by weight: 500-1,500 g of L-glycine, 1 000-3,000 g of L-alanine, 300-1,000 g of L-glutamic acid, 1,000-2,000 g of proline, 8,500-12,000 g of trimethyl glycine, 1,000-3,000 g of oxidized trimethylamine and 1,000-3000 g of squid organ meal. The invention has the advantages that the composite phagostimulant is free of overrich and over-fragrant stimulation, can be used for a long term, is safe and efficient and has no side effect; the raw materials of the feed phagostimulant are stable in source and simple in manufacturing technology and remarkably increase the feeding speed; the anti-stress capability of cultivating objects is remarkably improved while the food calling performance is enhanced, the cultivated jewfish has high survival rate and is brilliantly colored in body; and a majority of compositions in the phagostimulant are indispensable feed nutritional ingredients for growth of jewfish, so that the feed efficiency is improved, the feed coefficient is lowered and the cultivation cost is saved.