Kit and method for quantitatively detecting trimethylamine N-oxide

Abstract

Provided are a kit and method for quantitatively detecting trimethylamine N-oxide. The method for quantitatively detecting trimethylamine N-oxide comprise the following steps of adding trimethylamine N-oxide in an aqueous solution of human serum albumin to prepare a series of trimethylamine N-oxide standard solutions with a concentration gradient, adding an internal standard substance and a precipitating agent to each standard solution respectively, centrifuging the obtained standard solutions, obtaining a series of supernatant, analyzing the supernatant by using liquid chromatography and mass spectrometry respectively, and obtaining the ratio of the chromatographic peak area of trimethylamine N-oxide to that of the internal standard substance in each trimethylamine N-oxide standard solution and a relationship between the ratios and concentrations of trimethylamine N-oxide; adding the internal standard substance and the precipitating agent to a to-be-tested blood serum sample, conducting centrifugation, analyzing obtained supernatant by using liquid chromatography and mass spectrometry, obtaining the ratio of the chromatographic peak area of trimethylamine N-oxide to that of the internal standard substance in the to-be-tested blood serum sample, and obtaining the concentration of trimethylamine N-oxide in the to-be-tested blood serum sample according to the relationship between the ratios and the concentrations of trimethylamine N-oxide. According to the kit and method for quantitatively detecting trimethylamine N-oxide, the problem that unreliable detection results can be caused by application of water, a bovine serum albumin solution or a phosphate buffer solution and other blank biological matrix which are far from plasma components of the human body can be avoided, and the reliability of the detection results is improved.

Description

technical field [0001] The invention relates to the technical field of substance content detection, in particular to a trimethylamine oxide quantitative detection kit and method. Background technique [0002] The quantitative detection method of trimethylamine oxide (Trimetlylamine oxide, TMAO) in plasma has important clinical value. At present, many methods for the quantitative detection of trimethylamine oxide are used to detect the concentration of trimethylamine oxide in aquatic products, most of which are simple chromatography methods with limited sensitivity and accuracy. To detect the concentration of TMAO in biological samples such as plasma and serum, liquid chromatography-mass spectrometry (HPLC-MS) is currently the most commonly used method. Almost all human plasma contains TMAO, so it is difficult to obtain blank biological matrices during methodological verification. Many methods use blank biological matrices such as water, bovine serum albumin solution, and ph...

AI Technical Summary

Problems solved by technology

Almost all human plasma contains TMAO, so it is difficult to obtain blank biological matrices during methodological verification. Many methods use blank biological matrices such as water, bovine serum albumin solution, and phosphate buffered saline. The matrix components of the test samples are quite different, which leads to the low convincingness of the method validation and the unreliability of the test results obtained

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