Provided are a kit and method for quantitatively detecting
trimethylamine N-
oxide. The method for quantitatively detecting
trimethylamine N-
oxide comprise the following steps of adding
trimethylamine N-
oxide in an
aqueous solution of
human serum albumin to prepare a series of trimethylamine N-oxide standard solutions with a
concentration gradient, adding an
internal standard substance and a precipitating agent to each
standard solution respectively, centrifuging the obtained standard solutions, obtaining a series of supernatant, analyzing the supernatant by using liquid
chromatography and
mass spectrometry respectively, and obtaining the ratio of the chromatographic
peak area of trimethylamine N-oxide to that of the
internal standard substance in each trimethylamine N-oxide
standard solution and a relationship between the ratios and concentrations of trimethylamine N-oxide; adding the
internal standard substance and the precipitating agent to a to-be-tested
blood serum sample, conducting
centrifugation, analyzing obtained supernatant by using liquid
chromatography and
mass spectrometry, obtaining the ratio of the chromatographic
peak area of trimethylamine N-oxide to that of the internal standard substance in the to-be-tested
blood serum sample, and obtaining the concentration of trimethylamine N-oxide in the to-be-tested
blood serum sample according to the relationship between the ratios and the concentrations of trimethylamine N-oxide. According to the kit and method for quantitatively detecting trimethylamine N-oxide, the problem that unreliable detection results can be caused by application of water, a
bovine serum albumin solution or a
phosphate buffer solution and other blank biological matrix which are far from
plasma components of the
human body can be avoided, and the reliability of the detection results is improved.