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Cell cryopreservation liquid and preparation method and application thereof

A cryopreservation solution and cell technology, which is applied in the application, preservation of human or animal bodies, animal husbandry, etc., can solve the problems of cytotoxicity, stem cell damage, etc., and achieve simple and feasible operation, reasonable price, and ensure cell biological activity Effect

Active Publication Date: 2016-04-06
HENAN YINFENG BIOENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DMSO can be toxic to cells and cause irreversible damage to cryopreserved stem cells

Method used

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  • Cell cryopreservation liquid and preparation method and application thereof
  • Cell cryopreservation liquid and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 cell cryopreservation liquid

[0021] Dimethyl sulfoxide 8% w / v, epimedium total flavonoids 30ng / ml, polypeptide 1% w / v, bFGF 1% w / v, vitamin E 1% w / v, with DMEM medium and fetal bovine serum according to Dissolve at a ratio of 1:1, and set the volume to 100 ml to obtain the cell cryopreservation solution, wherein the sequence of the polypeptide is shown in SEQ ID NO:1.

Embodiment 2

[0022] The preparation of embodiment 2 stem cell cryopreservation liquid

[0023] Dimethyl sulfoxide 8% w / v, epimedium total flavonoids 30ng / ml, polypeptide 1% w / v, bFGF 1% w / v, vitamin E 1% w / v, with DMEM medium and fetal bovine serum according to Dissolve at a ratio of 1:1, and set the volume to 100ml to obtain the cell cryopreservation solution, wherein the sequence of the polypeptide is shown in SEQ ID NO:2.

Embodiment 3

[0024] The preparation of embodiment 3 stem cell cryopreservation liquid

[0025] Dimethyl sulfoxide 8% w / v, epimedium total flavonoids 30ng / ml, polypeptide 1% w / v, bFGF 1% w / v, vitamin E 1% w / v, with DMEM medium and fetal bovine serum according to Dissolve at a ratio of 1:1, and set the volume to 100ml to obtain the cell cryopreservation solution, wherein the sequence of the polypeptide is shown in SEQ ID NO: 1 and 2, which are obtained by mixing the two in equal proportions.

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Abstract

The invention provides cell cryopreservation liquid and a preparation method thereof. The cell cryopreservation liquid is prepared from, 8% w / v of dimethyl sulfoxide, 30 ng / ml of total flavonoids of herba epimedii, 1% w / v of polypeptide, 1% w / v of bFGF and 1% w / v of vitamin E. The cell cryopreservation liquid is used for cryopreserving culture cells, and especially the survival rate of the recovered cryopreserved cells is increased. The cell cryopreservation liquid has the advantages of being moderate in price and easy and convenient to operate and is suitable for being widely utilized.

Description

technical field [0001] The invention relates to the technical field of tissue cell culture, in particular to a cell cryopreservation solution and its preparation method and application. Background technique [0002] Neural stem cell (NeuralStemCell, NSC) is a kind of stem cell existing in embryonic or adult brain, spinal cord and other tissues. It is a kind of mother cell with division potential and self-renewal ability. It can differentiate into neurons, Various types of cells in nervous tissue such as astrocytes and oligodendrocytes can also be transdifferentiated into blood cells and skeletal muscle cells. In all nervous tissues such as the brain and spinal cord, different types of neural stem cells produce different types of progeny cells, and the distribution is also different. Although the cells are widely used, their source and quantity are still far from meeting the demand in order to be widely used in the field of clinical treatment. Therefore, large-scale expansi...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 张云国耿晓波朱学义赵明宇王小龙张雪梅
Owner HENAN YINFENG BIOENG CO LTD
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