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Cell cryopreservation agent of hematopoietic stem cells

A technology for hematopoietic stem cells and cryopreservation agents, which is applied in the field of hematopoietic stem cell cryopreservation agents and its preparation, can solve the problems of stem cell damage, cytotoxicity, etc., achieve the effect of reasonable price, simple and feasible operation, and ensure the biological activity of cells

Inactive Publication Date: 2017-09-15
陈印平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DMSO can be toxic to cells and cause irreversible damage to cryopreserved stem cells

Method used

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  • Cell cryopreservation agent of hematopoietic stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add glutamine 20mg, epidermal growth factor 300μg, 0.2% w / v sucrose, 0.5% w / v low-density lipoprotein, 0.5% w / v trehalose, 0.5% w / v lecithin, peptide 100ppm w / v, bFGF 0.6% w / v, vitamin E 0.5% w / v, glycerol 2% v / v, and finally use DMEM medium and fetal bovine serum to adjust the volume to 100ml according to the ratio of 1:1, the sequence of the polypeptide As shown in SEQ ID NO:1.

Embodiment 2

[0026] Add glutamine 20mg, epidermal growth factor 300μg, 0.2% w / v sucrose, 0.5% w / v low-density lipoprotein, 0.5% w / v trehalose, 0.5% w / v lecithin, peptide 100ppm w / v, bFGF 0.6% w / v, vitamin E 0.5% w / v, glycerol 2% v / v, and finally use DMEM medium and fetal bovine serum to adjust the volume to 100ml according to the ratio of 1:1, the sequence of the polypeptide As shown in SEQ ID NO:2.

Embodiment 3

[0028] Add glutamine 20mg, epidermal growth factor 300μg, 0.2% w / v sucrose, 0.5% w / v low-density lipoprotein, 0.5% w / v trehalose, 0.5% w / v lecithin, peptide 100ppm w / v, bFGF 0.6% w / v, vitamin E 0.5% w / v, glycerol 2% v / v, and finally use DMEM medium and fetal bovine serum to adjust the volume to 100ml according to the ratio of 1:1, the sequence of the polypeptide Shown in SEQ ID NO:3.

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Abstract

The invention provides a cell cryopreservation agent of hematopoietic stem cells. The cell cryopreservation agent comprises 20 mg of glutamine, 300 [mu]g of epidermal growth factors, 0.2% (w / v) of sucrose, 0.5% (w / v) of low density lipoproteins, 5% (w / v) of trehalose, 0.5% (w / v) of lecithin, 100 ppm (w / v) of polypeptides, 0.6% (w / v) of bFGF, 0.5% (w / v) of vitamin E and 2% (v / v) of glycerol, and a DMEM medium and fetal bovine serum are added to the cell cryopreservation agent according to a ratio of the DMEM medium to the fetal bovine serum of 1:1 until the volume is 100 ml. The glycerol, trehalose, vitamin E and polypeptides in the cryopreserved cells can clearly resist cell injuries brought by outside damages, the polypeptides also protect the cells from being damaged by ice crystals during freezing, and several other components are essential for maintaining the survival of specific hematopoietic cells. The cell cryopreservation agent is used for the cryopreservation of cultured cells, especially improves the survival rate of cryopreserved cells after resuscitation, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of tissue cell culture, in particular to a cryopreservation agent for hematopoietic stem cells and its preparation method and application. Background technique [0002] Hematopoietic stem cells are adult stem cells in the blood system. They are a heterogeneous group with the ability of long-term self-renewal and the potential to differentiate into various mature blood cells. It is a type of adult stem cell with the longest and most in-depth research history, and it has important guiding significance for the study of various stem cells, including tumor stem cells. [0003] In recent years. Autologous hematopoietic stem cell transplantation has been widely used in the treatment of malignant hematological diseases and various solid tumors. How to preserve the collected autologous hematopoietic stem cells with high quality is one of the keys to the success of AHSCT treatment. The preservation of AHSC is divide...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 陈印平
Owner 陈印平
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