Human serum albumin nano biomaterial and preparation method thereof
A technology of human serum albumin and nanofibers, applied in the direction of spinning solution preparation, single-component protein rayon, application, etc., to achieve the effect of simple production
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[0044] Example 1
[0045] To prepare a solution system for dissolving albumin: add 0.27ml of 0.1M ammonium hydroxide to 1.74ml of trifluoroethanol, then add 50ul of mercaptoethanol, stir to mix well. Weigh 0.3 g (10%, w / v) of genetically recombinant human serum albumin and dissolve in the above solution, gently stir with a magnetic rod for 30 minutes until the albumin is fully dissolved, and use a vacuum pump for 30 minutes to eliminate bubbles. Spinning solution.
[0046] The spinning solution passes through the spinneret at a flow rate of 0.3ml per hour, an electric field intensity of 1.0KV / cm, a distance between the electrode and the turntable 14cm, and the edge speed of the collector turntable is formed on a collector with 8 m / s. After being cured and dried in a glass drying dish (humidity 30%) for 24 hours, it becomes human albumin nanofibers.
[0047] The albumin fiber was soaked in deionized hot water at 37°C for 1 hour, soaked in 35% ethanol prepared with deionized water fo...
Example Embodiment
[0049] Example 2
[0050] Select human plasma source (non-gene recombination) albumin as the matrix material. To prepare a solution system for dissolving albumin: add 0.27ml of 0.1M ammonium hydroxide to 1.74ml of trifluoroethanol, then add 50ul of mercaptoethanol, stir to mix well. Weigh 0.3 g (10%, w / v) of genetically recombinant human serum albumin and dissolve in the above solution, gently stir with a magnetic rod for 30 minutes until the albumin is fully dissolved, and use a vacuum pump for 30 minutes to eliminate bubbles. Spinning solution.
[0051] The spinning solution passes through the spinneret at a flow rate of 0.3ml per hour, an electric field intensity of 1.0KV / cm, a distance between the electrode and the turntable 14cm, and the edge speed of the collector turntable is formed on a collector with 8 m / s. After being cured and dried in a glass drying dish (humidity 30%) for 24 hours, it becomes human albumin nanofibers.
[0052] The albumin fiber was soaked in deionized...
Example Embodiment
[0054] Example 3
[0055] To prepare a solution system for dissolving albumin: add 0.16ml of 0.1M ammonium hydroxide to 1.85ml of trifluoroethanol, then add 50ul of mercaptoethanol, stir to mix well. Weigh 0.37 g (12%, w / v) of genetically recombinant human serum albumin and dissolve in the above solution, gently stir with a magnetic rod for 30 minutes until the albumin is fully dissolved, and use a vacuum pump for 30 minutes to eliminate bubbles, and obtain Spinning solution.
[0056] The spinning solution is formed on a collector with a flow rate of 0.3ml per hour, an electric field intensity of 1.0KV / cm, a distance between the electrode and the turntable, and the edge speed of the collector turntable at 8 m / s through the spinneret. After being cured and dried in a glass drying dish (humidity 30%) for 24 hours, it becomes human albumin nanofibers.
[0057] The albumin fiber was soaked in deionized hot water at 37°C for 1 hour, soaked in 35% ethanol prepared with deionized water fo...
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