The invention relates to an integral rigid runner droplet digital PCR
system. The
system comprises a power device, a droplet generation device, a rigid thermal circulation runner, a thermal circulation
temperature control module, a fluorescent detection controller and a PC control module. A rigid thermal circulation pipeline has
high heat conducting performance, and a
rigid wall surface has low roughness and small relative deformation. The integral rigid runner droplet digital PCR
system can be used for online detecting a droplet in real time by adopting the droplet generation device with a
high loading capacity and a terminal fluorescent detection system, and has the characteristics of flexible
temperature control, safety, no need of a special PCR reaction
chip, high fluorescent detection flux, high sensitivity and the like; and the system can avoid the problems of measurement accuracy reduction and the like caused by droplet breakage and mutual fusion among droplets since the traditional droplet digital PCR equipment needs to manually transfer droplets in different reaction modules. The whole system forms a highly-
integrated design by droplet generation, PCR reaction thermal circulation and terminal droplet fluorescent real-time online detection.