Integral rigid runner droplet digital PCR system and method

An integrated, rigid technology, applied in chemical instruments and methods, biochemical equipment and methods, biochemical instruments, etc., to achieve the effects of reducing sample contamination, droplet fusion, and improving thermal conductivity
CN106520524AInactive Publication Date: 2017-03-22UNIV OF SCI & TECH OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
UNIV OF SCI & TECH OF CHINA
Publication Date
2017-03-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an integral rigid runner droplet digital PCR system. The system comprises a power device, a droplet generation device, a rigid thermal circulation runner, a thermal circulation temperature control module, a fluorescent detection controller and a PC control module. A rigid thermal circulation pipeline has high heat conducting performance, and a rigid wall surface has low roughness and small relative deformation. The integral rigid runner droplet digital PCR system can be used for online detecting a droplet in real time by adopting the droplet generation device with a high loading capacity and a terminal fluorescent detection system, and has the characteristics of flexible temperature control, safety, no need of a special PCR reaction chip, high fluorescent detection flux, high sensitivity and the like; and the system can avoid the problems of measurement accuracy reduction and the like caused by droplet breakage and mutual fusion among droplets since the traditional droplet digital PCR equipment needs to manually transfer droplets in different reaction modules. The whole system forms a highly-integrated design by droplet generation, PCR reaction thermal circulation and terminal droplet fluorescent real-time online detection.
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Description

technical field

[0001] The invention relates to an integrated rigid channel droplet digital PCR system and method, which belongs to the field of medical equipment and is suitable for polymerase chain reaction (PCR) requiring high throughput, on-line detection and complete quantification of amplified templates. Background technique

[0002] Polymerase chain reaction (PCR) is a very important method for nucleic acid analysis. Thanks to its ability to quickly enrich target molecules and prepare for the next step of analysis, it is widely used in the fields of medical and biological research, greatly accelerating The research process of biological genome structure. Due to the large volume of liquid, traditional PCR instruments require complex heating and cooling cycles, consume a lot of energy, lack real-time monitoring and cannot perform absolute quantitative analysis. Digital PCR technology is a new type of PCR technology that has developed rapidly in recent years, and it can...

Claims

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