Plasmid vector continuous lysis device

A cracking device and plasmid carrier technology, which is applied in the field of continuous cracking devices for plasmid carriers, can solve the problems of large differences, high manpower and material resources, and inability to achieve completely consistent bacterial cracking effects, so as to achieve the effect of ensuring stability and reducing time

Pending Publication Date: 2020-11-24
SHENZHEN PREGENE BIOPHARMA CO LTD
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, each manual operation produces inevitable differences, and the differences are relatively large, and the effect of each cell lysis cannot be completely consistent; and the operation of cell lysis takes a long time and consumes high manpower and material resources.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plasmid vector continuous lysis device
  • Plasmid vector continuous lysis device
  • Plasmid vector continuous lysis device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] It should be noted that if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. Implying their relative importance or implying the number of technical features indicated. Thus, the features defined as "first" and "second" may explicitly or implicitly include at least one of these features. In addition, the meaning of "and / or" appearing in the whole text includes three parallel schemes, taking "A and / or B" as an example, including scheme A, scheme B, or schemes that both A and B satisfy.

[0033] The invention provides a plasmid carrier continuous cracking device.

[0034] see figure 1 and figure 2 , the plasmid carrier continuous cracking device 100 comprises a reaction vessel 110 and a drive assembly 120; the reaction vessel 110 comprises a first vessel 111, a second vessel 112, a third vessel 113 and a fourth vessel 114, and the first vessel 111 For containing the resuspe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a plasmid vector continuous lysis device. The device comprises a reaction container and a driving assembly, wherein the reaction container comprises a first container, a secondcontainer, a third container and a fourth container; the first container is used for containing a resuspension turbid solution containing thalli and a resuspension solution; the second container is used for containing a lysis solution; the third container is used for containing a neutralization solution, and the fourth container is used for containing a lysis turbid solution containing the resuspension turbid solution and the lysis solution; the driving assembly comprises a first driving pump, a second driving pump and a third driving pump; the first driving pump is used for pumping the resuspension turbid solution in the first container into the fourth container, and the second driving pump is used for pumping the lysis solution in the second container into the fourth container; and thethird driving pump is used for pumping the lysis turbid solution in the fourth container into the third container. According to the plasmid vector continuous lysis device disclosed by the invention, the stability of thallus lysis is ensured, and the thallus lysis time is shortened.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a continuous cracking device for plasmid vectors. Background technique [0002] Extracting plasmids by lysing bacterial cells is a common technique in the field of biotechnology. In the traditional manual lysis method, the operator pours the resuspension solution into the container containing the bacteria, resuspends the bacteria and forms a resuspension cloudy solution, then adds the lysate solution to the container to form a lysis cloudy solution, and then quickly rotates the container , during the rotation process of the operator, the bacteria are continuously lysed by the lysate, releasing deoxyribonucleic acid (DeoxyriboNucleic Acid, DNA), ribonucleic acid (Ribonucleic Acid, RNA), host cell protein (Host Cell protein, HCP), host Cell DNA (Host Cell DNA, HCD) and carbohydrates and other substances. [0003] In order to ensure a better cell lysis effect, the operator needs to ro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/33C12M1/02C12M1/00
CPCC12N15/1003
Inventor 郑孟韬杜保平贾士闯丁怡瑾杨谦
Owner SHENZHEN PREGENE BIOPHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap