Plasmid vector continuous lysis device

Abstract

The invention discloses a plasmid vector continuous lysis device. The device comprises a reaction container and a driving assembly, wherein the reaction container comprises a first container, a secondcontainer, a third container and a fourth container; the first container is used for containing a resuspension turbid solution containing thalli and a resuspension solution; the second container is used for containing a lysis solution; the third container is used for containing a neutralization solution, and the fourth container is used for containing a lysis turbid solution containing the resuspension turbid solution and the lysis solution; the driving assembly comprises a first driving pump, a second driving pump and a third driving pump; the first driving pump is used for pumping the resuspension turbid solution in the first container into the fourth container, and the second driving pump is used for pumping the lysis solution in the second container into the fourth container; and thethird driving pump is used for pumping the lysis turbid solution in the fourth container into the third container. According to the plasmid vector continuous lysis device disclosed by the invention, the stability of thallus lysis is ensured, and the thallus lysis time is shortened.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a continuous cracking device for plasmid vectors. Background technique [0002] Extracting plasmids by lysing bacterial cells is a common technique in the field of biotechnology. In the traditional manual lysis method, the operator pours the resuspension solution into the container containing the bacteria, resuspends the bacteria and forms a resuspension cloudy solution, then adds the lysate solution to the container to form a lysis cloudy solution, and then quickly rotates the container , during the rotation process of the operator, the bacteria are continuously lysed by the lysate, releasing deoxyribonucleic acid (DeoxyriboNucleic Acid, DNA), ribonucleic acid (Ribonucleic Acid, RNA), host cell protein (Host Cell protein, HCP), host Cell DNA (Host Cell DNA, HCD) and carbohydrates and other substances. [0003] In order to ensure a better cell lysis effect, the operator needs to ro...

AI Technical Summary

Problems solved by technology

However, each manual operation produces inevitable differences, and the differences are relatively large, and the effect of each cell lysis cannot be completely consistent; and the operation of cell lysis takes a long time and consumes high manpower and material resources.

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