Fluorescence molecule imaging device

Abstract

The invention provides a fluorescence molecule imaging device, comprising an excitation module and an imaging module, wherein the excitation module consists of an exciting light source, an exciting filter wheel, an exciting optical filter and a plurality of beams of optical fibers. The imaging module consists of a CCD (Charge Coupled Device) camera, a lens, a lens adjusting unit, an emitting filter wheel, an emitting optical filter and an imaging cavity. The exciting light source generates exciting light, which passes through the exciting optical filter in the exciting filter wheel, and then the exciting light of some set wave band is irradiated to the detected object evenly via one optical fiber; the detected object is excited to generate the fluorescence signal with wavelength longer than that of the exciting light; the signal passes through the emitting optical filter of the emitting filter wheel set in front of the lens, then the specific emitting fluorescence signal is received by the CCD camera, converted into an image signal and displayed in a computer. The fluorescence molecule imaging device is simple in design and structure, convenient in operation, low in cost, and is widely applied to the fields such as medical image and the like.

Description

technical field [0001] The invention relates to an imaging device, in particular to a device for imaging fluorescent molecules by using excitation light of a specific wavelength band, and the device can be used to acquire surface information of a detected object. Background technique [0002] With the rapid development of genomics, proteomics and disease genomics, the diagnosis of diseases is developing from the traditional observation of disease characterization and routine biochemical experiment detection to the recognition of microscopic features at the gene and molecular levels. Technology can deeply understand the occurrence and development process of diseases from the level of genes and proteins, and can realize a holistic, continuous, non-invasive specific detection method that cannot be replaced by existing microscopic analysis. The theory of biological molecular imaging and its technology in vivo will provide a new Prevention, diagnosis and treatment tools. Compare...

AI Technical Summary

Problems solved by technology

This method has many disadvantages. For example, after the excitation light is reflected once, the intensity of the excitation light is greatly attenuated, and the ratio of useful light is very low.

In addition, the separation of the CCD from the lens will change the focal length and object distance of the lens, which will have a great impact on the light transmittance of the lens and the quantum efficiency of the camera.

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