37 results about "Polarized fluorescence" patented technology

A polarization fluorometer and a method for calibrating such a fluorometer. The fluorometer includes an excitation channel with an excitation polarizer for generating light polarized in a first plane or in a second plane to be conducted as a excitation light to a sample, an emission channel for conducting emission light from the sample, in which emission channel there is an emission polarization filter, an excitation detector, to which light polarized by the excitation polarizer can be conducted from the excitation channel, and a reference detector, to which light polarized by the excitation polarizer can be conducted from the excitation channel before being conducted to the sample or to the excitation detector. The reference detector can be calibrated by the excitation detector so that correction measurements can be carried out in real time during sample measurement. The fluorometer can be used especially for fluorescence spectra measurements.

The invention discloses a butachlor polarization fluorescence immunoassay method, mainly comprising the following steps: mixing the sample to be analyzed with the fluorescent-labeled hapten, adding the butachlor antibodies, directly measuring the polarization fluorescence signals of the reaction system, and calculating the butachlor concentration in the sample to be analyzed according to the standard curve set up by the butachlor standard sample. The method can be operated at room temperature, and the entire reaction time is very short; the method can simultaneously detect a plurality of samples in one step, without washing; after being equipped with a mechanical hand, the invention can realize sample adding and automated reading, which is a high-flux automated immunoassay method.

The invention discloses a preparation method of a cellulose nano paper for circular polarization fluorescence emission. The preparation method specifically comprises the following steps: step 1, synthesizing 4-(4-vinylbenzyl) acetophenone VBAP; step 2, synthesizing 1-(4-(4-vinylbenzyl)phenyl)-trifluorobutane-1,3-dione VBTF from 4-(4-vinylbenzyl) acetophenone prepared in the step 1; step 3, synthesizing a water-soluble polymer PVD from the product prepared in the step 2; step 4, synthesizing a water-soluble lanthanide polymer PVD-Eu from the product prepared in the step 3; and step 5, preparingthe cellulose nano paper for circular polarization fluorescence emission from the product prepared in the step 4. The water-soluble lanthanide polymer and cellulose nano crystals are assembled by adopting an EISA method to prepare a toughened and optically adjustable CPL nano material.

A suspended particle flux measurement device using synchronous polarization and fluorescence, including a light source, a polarizer, an illumination optical system, a first receiving optical system, a second receiving optical system, a polarization imaging system, a fluorescence camera, and an image processing terminal, the light source The emitted light passes through the polarizer to generate incident polarized light, which is converted into a sheet light source through the illumination optical system, and illuminates multiple suspended particles in a liquid section. The first and second receiving optical systems are set at two symmetrical scattering angles , receive the scattered light of the suspended particles and send them to the polarization imaging system and the fluorescence camera respectively, so that each suspended particle forms a polarization image point and a fluorescence image point on the polarization imaging system and the fluorescence camera respectively, realizing multiple For the measurement of the flux of suspended particulate matter, the polarization-fluorescence synchronous measurement data of each suspended particulate matter is used to determine the type information of the suspended particulate matter. The device can quickly and high-throughput identify suspended particulate matter in water.

The invention provides synthesis of a binuclear liquid crystal polarized cyclometalated platinum complex by using 2,7-bi[4'-(4''-alkoxymethyl-2''-pyridine)-phenyl]-9,9-bi(dodecyl) fluorene as cyclomedtalating ligand and bi[(4-dodecoxy) benzoyl]methane as anionic ligand, and research of polarized fluorescence and liquid crystal performance thereof. In the method, alkoxymethyl is introduced to the 5-position of a pyridine ring of the traditional cyclomedtalating ligand 2-phenylpyridine molecule; a C N cyclomedtalating ligand of double alkoxy chain with dual coordination center and liquid crystal performance through fluorene bridge connection is constructed; a novel binuclear cyclometalated platinum complex is synthesized by using the bi[(4-dodecoxy) benzoyl]methane as the anionic ligand andthe 2,7-bi[4'-(4''-alkoxymethyl-2''-pyridine)-phenyl]-9,9-bi(dodecyl) fluorene liquid crystal material as the cyclomedtalating ligand. Compared with the traditional cyclometalated platinum luminescence material using the functional phenylpyridine as the cyclomedtalating ligand, the binuclear liquid crystal polarized cyclometalated platinum complex not only has high-efficiency luminescence performance, but also has good liquid crystal performance and polarized fluorescence performance. The binuclear liquid crystal polarized cyclometalated platinum complex of the invention provides a new way for obtaining the organic electrophosphorescent material with liquid crystal polarization.

The invention discloses a butachlor polarization fluorescence immunoassay method, mainly comprising the following steps: mixing the sample to be analyzed with the fluorescent-labeled hapten, adding the butachlor antibodies, directly measuring the polarization fluorescence signals of the reaction system, and calculating the butachlor concentration in the sample to be analyzed according to the standard curve set up by the butachlor standard sample. The method can be operated at room temperature, and the entire reaction time is very short; the method can simultaneously detect a plurality of samples in one step, without washing; after being equipped with a mechanical hand, the invention can realize sample adding and automated reading, which is a high-flux automated immunoassay method.

The invention discloses a chiral nanorod film with circular polarization fluorescence, the chiral nanorod film is formed by sequentially staggering and superposing a plurality of laminations clockwise or anticlockwise, and a preparation method of the chiral nanorod film comprises the following steps: (1) dispersing nanorods in n-hexane, and then dropwise adding to the surface of a water phase in a groove of a film drawing machine; (2) compressing a sliding barrier to the middle at a preset speed to preset surface pressure, and forming a nanorod film on the surface of a water phase; (3) transferring the nanorod film to a carrier by adopting a horizontal film forming mode, and drying; and (4) repeating the operation of the steps (1)-(3) to form a film, transferring, drying and overlapping to obtain the chiral nanorod film with circular polarization fluorescence. According to the method, the uniform and flat large-area chiral nanorod film with a high luminescence asymmetry factor (glum) can be prepared; in addition, the glum value of the chiral nanorod film can be regulated and controlled by controlling the rotation angle of the carrier and the number of layers of the film.

The invention discloses a preparation method of circularly polarized fluorescent emission cellulose nano paper, specifically comprising the following steps: step 1, synthesizing 4-(4-vinylbenzyl)acetophenone VBAP; step 2, preparing according to step 1 Synthetic 1-(4-(4-vinylbenzyl) phenyl)-trifluorobutane-1,3-diketone VBTF of 4-(4-vinylbenzyl) acetophenone; Step 3, according to step 2. Synthesize the water-soluble polymer PVD from the prepared product; step 4, synthesize the water-soluble lanthanide polymer PVD-Eu according to the product obtained in step 3; step 5, prepare circularly polarized fluorescence-emitting cellulose nanopaper based on the product obtained in step 4. The invention adopts the EISA method to co-assemble the water-soluble lanthanide polymer and the cellulose nano crystal to prepare the toughened and optically adjustable CPL nano material.

The invention pertains to a method of obtaining structural and functional information on proteins, based on polarization fluorescence microscopy, which comprises subjecting a protein tagged with a fluorophore to two- or multi-photon fluorescence microscopy, whereas the observed protein is irradiated with a laser beam with light of at least two different polarizations, which excites the fluorescence of the fluorophore, and wherein information on localization, intensities and polarizations of the fluorescence excited by the different polarizations of the excitation laser beam is used to identify, localize and quantify anisotropy of absorption and / or fluorescence, which information is then used to infer structural and functional properties of proteins. An example of a device for obtaining structural and functional information on proteins, based on polarization fluorescence microscopy, comprises a modulator (P) for rapid modulation of the excitation beam (1) for eliciting two- or multi-photon fluorescence, and a control unit (R), wherein the function of the modulator (P) and control unit (R) is synchronized with scanning of the microscope (M) such that information on fluorescence intensity acquired by the microscope (M) is attributable to a particular polarization state of the excitation beam (1) by virtue of knowing the temporal profile of the polarization modulation of the excitation beam (1) effected by the modulator (P). The method and device of the invention allow determining and monitoring structure and function of proteins, such as membrane proteins, and thereby observing physiological processes in living cells.

The invention discloses a device which has a special pre-sharpening processing unit and can automatically perform early diagnosis and treatment effect inspection on cancer. The device integrates a specific fluorescence spectrometer, polarizing devices with different characteristics, a refrigerated centrifuge, a specific diluter and a computer with various software, etc. It can detect the fluorescence spectrum and characteristic spectrum of the patient's blood and their changing characteristics. Including the 515nm main peak of the fluorescence spectrum and the 624nm characteristic peak intensity ratio, the normalized half-peak width of the main peak, the parallel-perpendicular, vertical-parallel, parallel-parallel and vertical-vertical normalized intensity values ​​of the polarized fluorescence spectrum, the degree of polarization of serum, anisotropy Indexes such as heterosexual value and viscosity are used to realize early diagnosis of cancer and check the effect of treatment. The feature of this device is that the above-mentioned five major devices are integrated into one instrument, which can make early diagnosis of various cancers. The diagnosis principle is clear, the operation is convenient, the precision is high, the speed is fast and the cost is low, and the in vitro detection is non-invasive.

The invention discloses novel dichromatic dye as well as a preparation method and a purpose thereof. A material is synthesized by adopting a Suzuki reaction, a hydrolysis reaction and a substitution reaction, is excellent dichromatic dye, has high quantum yield and dichromatic ratio, further, a molecular long axis is parallel to a dipole moment, and has a polymerizable terminal. The novel dichromatic dye can be used for obtaining high-polarization fluorescent light or preparing high-polarization absorption and fluorescent emission films. Thus, the material as well as the preparation method and the purpose thereof have wide application prospects in the fields of display techniques and polarization optics.

The invention discloses a produciton method of a two-dimensional crystal forbidden band fluorescence polarization modulation device. The method comprises the following steps: providing a high-conductivity material substrate; oxidizing an insulating layer on the high-conductivity material substrate; depositing a two-dimensional crystal single layer on the insulating layer; evaporating a light-transmitting dielectric layer on the two-dimensional crystal single layer; spin-coating electron beam negative photoresist on the light-transmitting dielectric layer; carrying out electron beam exposure and development on the negative photoresist to obtain a nano-structure pattern on the negative photoresist; evaporating a metal layer on the developed negative photoresist; and removing the residual negative photoresist to obtain a metal nano metamaterial with a periodic unit structure on the light-transmitting dielectric layer. According to the device for modulating polarization of two-dimensionalcrystal forbidden band fluorescence, an anisotropic resonance field of the metal nano metamaterial is used for regulating and controlling a semiconductor fluorescence field, polarized fluorescence isachieved, a purpose of polarization adjustability is achieved, and a linear adjustable range and a temperature application range of the two-dimensional crystal forbidden band fluorescence are effectively widened.

The present invention provides a simple and direct method for the simultaneous correction of steady-state polarized fluorescence intensities, depolarized (or scrambled) by the effects of applied hydrostatic pressure without having to first determine the scrambling factors from a separate experiment. The method involves direct recalculation of the measured polarized data obtained for the sample of interest at the time of data collection.