37 results about "Polarized fluorescence" patented technology

The invention relates to a high-accuracy fluorescence anisotropy microscopic imaging device and method. The imaging method comprises the following steps of: on a reflective fluorescence microscope, leading lights emitted by a light source to pass through a polarizer, an excitation filter plate and a spectroscope, and then focusing the lights on a sample; leading fluorescence reflected by the sample to pass through an objective lens, the spectroscope and an emission filter plate, and then imaging on an image plane of a CCD (Charge-Coupled Device) camera through an adjustable angle analyzer; controlling the adjustable angle analyzer to rotate around an optical axis of an imaging optical path step by step by using a computer, wherein when the adjustable angle analyzer rotates by an angle every time, the CCD camera shoots a frame of polarized fluorescence image, M images are totally shot; and finally calculating fluorescence anisotropic images from the M images by using Fourier transform.The imaging method is characterized in that the measuring resolution ratio of the fluorescence anisotropy is greatly improved by establishing a single-channel fluorescence analyzing optical path; andthe microscopic imaging system has higher stability and better anti-jamming capability.

The invention provides synthesis of a binuclear liquid crystal polarized cyclometalated platinum complex by using 2,7-bi[4'-(4''-alkoxymethyl-2''-pyridine)-phenyl]-9,9-bi(dodecyl) fluorene as cyclomedtalating ligand and bi[(4-dodecoxy) benzoyl]methane as anionic ligand, and research of polarized fluorescence and liquid crystal performance thereof. In the method, alkoxymethyl is introduced to the 5-position of a pyridine ring of the traditional cyclomedtalating ligand 2-phenylpyridine molecule; a C N cyclomedtalating ligand of double alkoxy chain with dual coordination center and liquid crystal performance through fluorene bridge connection is constructed; a novel binuclear cyclometalated platinum complex is synthesized by using the bi[(4-dodecoxy) benzoyl]methane as the anionic ligand and the 2,7-bi[4'-(4''-alkoxymethyl-2''-pyridine)-phenyl]-9,9-bi(dodecyl) fluorene liquid crystal material as the cyclomedtalating ligand. Compared with the traditional cyclometalated platinum luminescence material using the functional phenylpyridine as the cyclomedtalating ligand, the binuclear liquid crystal polarized cyclometalated platinum complex not only has high-efficiency luminescence performance, but also has good liquid crystal performance and polarized fluorescence performance. The binuclear liquid crystal polarized cyclometalated platinum complex of the invention provides a new way for obtaining the organic electrophosphorescent material with liquid crystal polarization.

The present invention is directed to a novel multi-wavelength imaging method and apparatus that enables rapid imaging of tissue regions with accurate identification of tissue types within the region. Optical properties, such as co-polarized or cross-polarized fluorescence or reflectance intensity, optical density and / or reflectance, can be determined at a plurality of locations within the tissue region for each wavelength. Said properties at the two wavelengths, including calculated derivatives of the optical property with respect to wavelength, can be analyzed to image tissue structures and identify tissue types within the tissue region more accurately than can be achieved based on properties measured at a single wavelength.

The invention discloses a suspended particulate matter flux measuring device utilizing synchronous polarization and fluorescence. The system comprises a light source, a polarizer, an irradiation optical system, a first receiving optical system, a second receiving optical system, a polarization imaging system, a fluorescence camera and an image processing end, wherein light emitted by the light source passes through the polarizer and then generates the incident polarized light, is converted into a sheet light source through an irradiation optical system, irradiates a plurality of suspended particles in a liquid section, the first receiving optical system and the second receiving optical system are arranged at two symmetrical scattering angles, the scattered light of the suspended particulate matter is received and sent to the polarization imaging system and the fluorescence camera so that each suspended particulate matter forms a polarization image point and a fluorescence image point on the polarization imaging system and the fluorescence camera at the same time, flux measurement of multiple suspended particulate matters in a water body section is realized, and the type informationof the suspended particulate matters is determined by utilizing polarization-fluorescence synchronous measurement data of each suspended particulate matters. The system is advantaged in that the system can quickly identify the suspended particulate matters in a water body at high flux.

The invention provides a wavelength / angle modulation free conversion polarized fluorescence imaging surface plasmon resonance instrument comprising a polarized upright fluorescence microscopy opticalpath, a sample three-dimensional stage, a cage incident optical path, a cage reflected optical path, an electromechanical turning device, a data processing system and a syringe pump, wherein the sample three-dimensional stage is integrated with a right-angle prism, a gold-plated sample substrate and a multi-channel microfluidic flow cell; and the liquid inlet and outlet of the multi-channel microfluidic flow cell are respectively connected to the liquid outlet and inlet of the syringe pump through pipelines. The wavelength / angle modulation free conversion polarized fluorescence imaging surfaceplasmon resonance instrument provided by the invention can realize real-time observation through polarized fluorescence imaging at the same time when performing high-sensitivity quantitative and qualitative wavelength angle modulation free conversion surface plasmon resonance detection on the interaction between anisotropic biological samples, and the refractive index changes up to 1.0*10-5, thedetection sensitivity reaches 10-11g / ml, and the image resolution is up to 2um. The wavelength / angle modulation free conversion polarized fluorescence imaging surface plasmon resonance instrument provided by the invention takes into account the standing balance training modes at all levels, and satisfies the needs of the patient group for standing balance and stable training at all stages.

This invention relates to a fiber polarized super fluorescence light source including: setting a first broadband reflector, a first doped fiber, a first double-color plate, an optical isolator, a first polarized beam-splitter, a Faraday rotatory device, a second polarized beam-splitter, a second double color plates, a second doped fiber, 1 / 4 wave plate and a second broadband reflector on a same optical axis, the included angle of the first and second double color plates and said optical axis is 45deg., a first pump light source is set at th cross point of the first double color plate and said optical axis and vertical to the optical axis, a second pump light source is set at the cross point of the second double color plate and the axis and vertical to the axis, a third broadband reflector is set at the cross point of the second polarized beam splitter and said axis and vertical to the axis direction and the direction vertical to the axis at the cross point of the first polarized beam splitter and the axis is the output direction of the polarized fluorescence light.

A polarization fluorometer and a method for calibrating such a fluorometer. The fluorometer includes an excitation channel with an excitation polarizer for generating light polarized in a first plane or in a second plane to be conducted as a excitation light to a sample, an emission channel for conducting emission light from the sample, in which emission channel there is an emission polarization filter, an excitation detector, to which light polarized by the excitation polarizer can be conducted from the excitation channel, and a reference detector, to which light polarized by the excitation polarizer can be conducted from the excitation channel before being conducted to the sample or to the excitation detector. The reference detector can be calibrated by the excitation detector so that correction measurements can be carried out in real time during sample measurement. The fluorometer can be used especially for fluorescence spectra measurements.

The invention discloses a butachlor polarization fluorescence immunoassay method, mainly comprising the following steps: mixing the sample to be analyzed with the fluorescent-labeled hapten, adding the butachlor antibodies, directly measuring the polarization fluorescence signals of the reaction system, and calculating the butachlor concentration in the sample to be analyzed according to the standard curve set up by the butachlor standard sample. The method can be operated at room temperature, and the entire reaction time is very short; the method can simultaneously detect a plurality of samples in one step, without washing; after being equipped with a mechanical hand, the invention can realize sample adding and automated reading, which is a high-flux automated immunoassay method.

A method for evaluating an orientation state of an oriented layer, comprising: applying, on the oriented layer, a solution of a light emitting polymer containing a fluorescent material that is capable of being oriented according to an orientation state of an underlying layer; performing a heat treatment on the applied solution of the light emitting polymer to form a light emitting polymer film; obtaining a polarized fluorescence spectrum of the light emitting polymer film; determining an orientation parameter using an orientation function equation based on the polarized fluorescence spectrum; and evaluating the orientation state of the oriented layer using the orientation parameter.

The invention discloses an intelligent regulation circular polarization fluorescent perylene derivative photoelectric material, which has the following chemical structural formula defined in the specification, wherein the substituted functional group R is any one of the following structures in the structural formula, R1 is a C1-C12 linear chain or branched chain alkyl or alkoxy chain, and *1 or *2or* 3 is a coupling position. According to the present invention, the chromophore using perylene as the core and having the side chain of the chiral inducing functional group and other chromophores are subjected to the Suzuki coupling reaction to construct a class of the photoelectric materials with the circular polarization fluorescence, wherein the class of the CPL materials have the followingadvantages that the intelligent CPL signal regulation is achieved in the case of the external stimuli response, the CPL luminescence of the single molecule and the dimer is achieved by regulating theconcentration in the good solvent tetrahydrofuran, the aggregation-induced CPL intelligent regulation is achieved in the tetrahydrofuran-water system, and especially the un-annealed film has a good CPL signal.

The invention discloses an adiponectin receptor ligand screening method which uses the polarized fluorescence technology for screening adiponectin receptor ligands. The invention further discloses an adiponectin receptor agonist screening method including the steps: taking a to-be-detected sample, adopting the adiponectin receptor ligand screening method for screening, and screening by the aid of the cell culture immune polarized fluorescence technology. The adiponectin receptor ligand screening method and the adiponectin receptor agonist screening method can accurately and effectively screen the adiponectin receptor ligands and adiponectin receptor agonists, lay a good foundation for screening anti-inflammation, antidiabetic and atherosclerotic drugs and have bright industrial application prospect.

The invention discloses a preparation method of a cellulose nano paper for circular polarization fluorescence emission. The preparation method specifically comprises the following steps: step 1, synthesizing 4-(4-vinylbenzyl) acetophenone VBAP; step 2, synthesizing 1-(4-(4-vinylbenzyl)phenyl)-trifluorobutane-1,3-dione VBTF from 4-(4-vinylbenzyl) acetophenone prepared in the step 1; step 3, synthesizing a water-soluble polymer PVD from the product prepared in the step 2; step 4, synthesizing a water-soluble lanthanide polymer PVD-Eu from the product prepared in the step 3; and step 5, preparingthe cellulose nano paper for circular polarization fluorescence emission from the product prepared in the step 4. The water-soluble lanthanide polymer and cellulose nano crystals are assembled by adopting an EISA method to prepare a toughened and optically adjustable CPL nano material.

The invention discloses a device and a method for modulating forbidden band fluorescence polarization of a two-dimensional crystal. The device comprises a high-conductivity material substrate, an insulating layer formed on the high-conductivity material substrate, a two-dimensional crystal monolayer formed on the insulating layer, a light-transmitting dielectric layer formed on the two-dimensionalcrystal monolayer, and a metal nano metamaterial with a periodic unit structure formed on the light-transmitting dielectric layer. Based on a metal surface plasmon resonance principle, a semiconductor fluorescence field is regulated and controlled by utilizing an anisotropic resonance field of the metal nano metamaterial so that polarized fluorescence is realized, and a purpose of polarization adjustability is achieved. According to the device and the method for modulating the polarization of the two-dimensional crystal forbidden band fluorescence, a linear adjustable range and a temperatureapplication range of the two-dimensional crystal forbidden band fluorescence are effectively widened.

A method of determining a concentration of analytes of interest in a sample reaction mixture is disclosed. The method can include measuring intensities of the polarized fluorescence of at least one comparative reaction mixture containing different, known amounts of the analytes of interest, and measuring the intensities of the polarized fluorescence of a sample reaction mixture. The method can also include determining the preliminary concentrations of the analytes of interest in the sample reaction mixture by comparing the measured intensities of the sample reaction mixtures at various time points. The margin of error for the preliminary concentration of the analytes of interest in the sample can be determined at the various time points. Finally, the concentration of the analytes of interest in the sample reaction mixture can be determined by comparing the preliminary concentrations and the respective margin of error at the given time points.

A suspended particle flux measurement device using synchronous polarization and fluorescence, including a light source, a polarizer, an illumination optical system, a first receiving optical system, a second receiving optical system, a polarization imaging system, a fluorescence camera, and an image processing terminal, the light source The emitted light passes through the polarizer to generate incident polarized light, which is converted into a sheet light source through the illumination optical system, and illuminates multiple suspended particles in a liquid section. The first and second receiving optical systems are set at two symmetrical scattering angles , receive the scattered light of the suspended particles and send them to the polarization imaging system and the fluorescence camera respectively, so that each suspended particle forms a polarization image point and a fluorescence image point on the polarization imaging system and the fluorescence camera respectively, realizing multiple For the measurement of the flux of suspended particulate matter, the polarization-fluorescence synchronous measurement data of each suspended particulate matter is used to determine the type information of the suspended particulate matter. The device can quickly and high-throughput identify suspended particulate matter in water.

The invention discloses a polarization resolution fluorescence test system based on a three-dimensional movable light path, which comprises a laser generation assembly used for generating exciting light; the laser polarization adjusting assembly is used for adjusting the polarization state of the exciting light; the optical filter is used for reflecting the exciting light and transmitting fluorescent light emitted by a sample; the three-dimensional independent light path displacement assembly is used for adjusting a light path in a three-dimensional manner so as to focus a light spot to independently move on the surface of the sample on the x axis, the y axis and the z axis; the microscope system is used for carrying out illumination and microscopic imaging on the surface appearance, the exciting light and the fluorescent light of the sample; the fluorescence polarization adjusting assembly is used for adjusting the polarization state of fluorescence; and the spectrum detection module is used for detecting the spectrum and the service life of the fluorescence. The device has the characteristics that a sample does not move and a light path moves, can be compatible with most non-displaceable cryostats on the market, and realizes any circular polarization or linear polarization fluorescence detection by adopting polarization adjustable components and a small-angle incidence mode.

The invention provides synthesis of a binuclear liquid crystal polarized cyclometalated platinum complex by using 2,7-bi[4'-(4''-alkoxymethyl-2''-pyridine)-phenyl]-9,9-bi(dodecyl) fluorene as cyclomedtalating ligand and bi[(4-dodecoxy) benzoyl]methane as anionic ligand, and research of polarized fluorescence and liquid crystal performance thereof. In the method, alkoxymethyl is introduced to the 5-position of a pyridine ring of the traditional cyclomedtalating ligand 2-phenylpyridine molecule; a C N cyclomedtalating ligand of double alkoxy chain with dual coordination center and liquid crystal performance through fluorene bridge connection is constructed; a novel binuclear cyclometalated platinum complex is synthesized by using the bi[(4-dodecoxy) benzoyl]methane as the anionic ligand andthe 2,7-bi[4'-(4''-alkoxymethyl-2''-pyridine)-phenyl]-9,9-bi(dodecyl) fluorene liquid crystal material as the cyclomedtalating ligand. Compared with the traditional cyclometalated platinum luminescence material using the functional phenylpyridine as the cyclomedtalating ligand, the binuclear liquid crystal polarized cyclometalated platinum complex not only has high-efficiency luminescence performance, but also has good liquid crystal performance and polarized fluorescence performance. The binuclear liquid crystal polarized cyclometalated platinum complex of the invention provides a new way for obtaining the organic electrophosphorescent material with liquid crystal polarization.

The invention discloses a butachlor polarization fluorescence immunoassay method, mainly comprising the following steps: mixing the sample to be analyzed with the fluorescent-labeled hapten, adding the butachlor antibodies, directly measuring the polarization fluorescence signals of the reaction system, and calculating the butachlor concentration in the sample to be analyzed according to the standard curve set up by the butachlor standard sample. The method can be operated at room temperature, and the entire reaction time is very short; the method can simultaneously detect a plurality of samples in one step, without washing; after being equipped with a mechanical hand, the invention can realize sample adding and automated reading, which is a high-flux automated immunoassay method.

The invention discloses a chiral nanorod film with circular polarization fluorescence, the chiral nanorod film is formed by sequentially staggering and superposing a plurality of laminations clockwise or anticlockwise, and a preparation method of the chiral nanorod film comprises the following steps: (1) dispersing nanorods in n-hexane, and then dropwise adding to the surface of a water phase in a groove of a film drawing machine; (2) compressing a sliding barrier to the middle at a preset speed to preset surface pressure, and forming a nanorod film on the surface of a water phase; (3) transferring the nanorod film to a carrier by adopting a horizontal film forming mode, and drying; and (4) repeating the operation of the steps (1)-(3) to form a film, transferring, drying and overlapping to obtain the chiral nanorod film with circular polarization fluorescence. According to the method, the uniform and flat large-area chiral nanorod film with a high luminescence asymmetry factor (glum) can be prepared; in addition, the glum value of the chiral nanorod film can be regulated and controlled by controlling the rotation angle of the carrier and the number of layers of the film.

The invention relates to the field of nano materials, in particular to a circularly polarized luminous chiral rare earth phosphate nano material and a preparation method and application thereof, and the preparation method comprises the following steps: S1, preparing a rare earth reaction precursor solution and a phosphate ion reaction precursor solution; s2, adding the phosphate ion reaction precursor solution into the rare earth reaction precursor solution to obtain a mixed solution; s3, carrying out heat preservation on the mixed solution until a mixed turbid solution is obtained; and S4, centrifugally washing the mixed turbid liquid to obtain the chiral rare earth phosphate nanocrystal with the circularly polarized light-emitting property. According to the invention, a rare earth inorganic compound and chiral molecules are combined to synthesize the chiral rare earth phosphate nanocrystal with circular polarization fluorescence property. The simple and efficient coprecipitation method is adopted, operation is easy, reaction conditions are easy to achieve, cost is low, and good universality is achieved.

The invention pertains to a method of obtaining structural and functional information on proteins, based on polarization fluorescence microscopy, which comprises subjecting a protein tagged with a fluorophore to two- or multi-photon fluorescence microscopy, whereas the observed protein is irradiated with a laser beam with light of at least two different polarizations, which excites the fluorescence of the fluorophore, and wherein information on localization, intensities and polarizations of the fluorescence excited by the different polarizations of the excitation laser beam is used to identify, localize and quantify anisotropy of absorption and / or fluorescence, which information is then used to infer structural and functional properties of proteins. An example of a device for obtaining structural and functional information on proteins, based on polarization fluorescence microscopy, comprises a modulator (P) for rapid modulation of the excitation beam (1) for eliciting two- or multi-photon fluorescence, and a control unit (R), wherein the function of the modulator (P) and control unit (R) is synchronized with scanning of the microscope (M) such that information on fluorescence intensity acquired by the microscope (M) is attributable to a particular polarization state of the excitation beam (1) by virtue of knowing the temporal profile of the polarization modulation of the excitation beam (1) effected by the modulator (P). The method and device of the invention allow determining and monitoring structure and function of proteins, such as membrane proteins, and thereby observing physiological processes in living cells.

The present invention provides a simple and direct method for the simultaneous correction of steady-state polarized fluorescence intensities, depolarized (or scrambled) by the effects of applied hydrostatic pressure without having to first determine the scrambling factors from a separate experiment. The method involves direct recalculation of the measured polarized data obtained for the sample of interest at the time of data collection.

The invention relates to a microscopic circular polarization fluorescence spectrum detection system and method based on a single photon counting method. The system is characterized in that a white light source, a detected sample, a dichroscope, a photoelastic modulator and a Glan prism are arranged in a line, and light emitted by a switchable light source system enters the dichroscope through a depolarizer; the detection switching reflecting mirror is movably arranged between the dichroscope and the photoelastic modulator, when the detection switching reflecting mirror moves in, emergent light of the dichroscope is reflected by the detection switching reflecting mirror to enter the microscopic imaging system, and when the detection switching reflecting mirror moves out, emergent light of the dichroscope enters the spectrum acquisition system through the photoelastic modulator and the Glan prism, the photoelastic modulator is connected with the photoelastic modulator controller through a line, and the photoelastic modulator controller is connected with the spectrum acquisition system through a line. According to the invention, light source and system conversion can be realized as required, and circular polarization fluorescence spectrum detection of solid samples, especially high-voltage module samples, can be realized.

The invention discloses a device which has a special pre-sharpening processing unit and can automatically perform early diagnosis and treatment effect inspection on cancer. The device integrates a specific fluorescence spectrometer, polarizing devices with different characteristics, a refrigerated centrifuge, a specific diluter and a computer with various software, etc. It can detect the fluorescence spectrum and characteristic spectrum of the patient's blood and their changing characteristics. Including the 515nm main peak of the fluorescence spectrum and the 624nm characteristic peak intensity ratio, the normalized half-peak width of the main peak, the parallel-perpendicular, vertical-parallel, parallel-parallel and vertical-vertical normalized intensity values ​​of the polarized fluorescence spectrum, the degree of polarization of serum, anisotropy Indexes such as heterosexual value and viscosity are used to realize early diagnosis of cancer and check the effect of treatment. The feature of this device is that the above-mentioned five major devices are integrated into one instrument, which can make early diagnosis of various cancers. The diagnosis principle is clear, the operation is convenient, the precision is high, the speed is fast and the cost is low, and the in vitro detection is non-invasive.

The invention discloses novel dichromatic dye as well as a preparation method and a purpose thereof. A material is synthesized by adopting a Suzuki reaction, a hydrolysis reaction and a substitution reaction, is excellent dichromatic dye, has high quantum yield and dichromatic ratio, further, a molecular long axis is parallel to a dipole moment, and has a polymerizable terminal. The novel dichromatic dye can be used for obtaining high-polarization fluorescent light or preparing high-polarization absorption and fluorescent emission films. Thus, the material as well as the preparation method and the purpose thereof have wide application prospects in the fields of display techniques and polarization optics.

The present invention relates to materials with strong circular dichroism (CD), circularly polarized luminescence (CPL), and high fluorescence quantum yield in the aggregate state and methods of preparation and use thereof.

The invention provides ink with multiple anti-counterfeiting effects. The ink is prepared from the following raw materials in parts by weight: 3 parts of molecules with macroscopic co-assembly capability; 1-2 parts by weight of an organic chiral dopant; 0.1 part by weight to 0.3 part by weight of an inorganic luminescent material; and the inorganic luminescent material is a high-quality II-VI group quantum dot or a high-quality III-V group quantum dot. Compared with the prior art, the ink with multiple anti-counterfeiting effects provided by the invention adopts components with specific content, realizes relatively good overall interaction, has selectively reflective structural colors, can observe different effects under left and right polaroids respectively, can emit circularly polarized fluorescence under the excitation of ultraviolet light (such as a 365nm ultraviolet lamp), and has the advantages of high anti-counterfeiting effect, high anti-counterfeiting effect and the like. The fluorescent light has a large asymmetric factor, can present an obvious brightness difference under a left / right polaroid, and can respond to temperature change to lose structural color, and in addition, an obvious blue shift phenomenon can be observed along with inclination of an observation angle.

The invention discloses a produciton method of a two-dimensional crystal forbidden band fluorescence polarization modulation device. The method comprises the following steps: providing a high-conductivity material substrate; oxidizing an insulating layer on the high-conductivity material substrate; depositing a two-dimensional crystal single layer on the insulating layer; evaporating a light-transmitting dielectric layer on the two-dimensional crystal single layer; spin-coating electron beam negative photoresist on the light-transmitting dielectric layer; carrying out electron beam exposure and development on the negative photoresist to obtain a nano-structure pattern on the negative photoresist; evaporating a metal layer on the developed negative photoresist; and removing the residual negative photoresist to obtain a metal nano metamaterial with a periodic unit structure on the light-transmitting dielectric layer. According to the device for modulating polarization of two-dimensionalcrystal forbidden band fluorescence, an anisotropic resonance field of the metal nano metamaterial is used for regulating and controlling a semiconductor fluorescence field, polarized fluorescence isachieved, a purpose of polarization adjustability is achieved, and a linear adjustable range and a temperature application range of the two-dimensional crystal forbidden band fluorescence are effectively widened.

The present invention provides a simple and direct method for the simultaneous correction of steady-state polarized fluorescence intensities, depolarized (or scrambled) by the effects of applied hydrostatic pressure without having to first determine the scrambling factors from a separate experiment. The method involves direct recalculation of the measured polarized data obtained for the sample of interest at the time of data collection.