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34 results about "Fluorescence anisotropy" patented technology
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Fluorescence anisotropy or fluorescence polarization is the phenomenon where the light emitted by a fluorophore has unequal intensities along different axes of polarization. Early pioneers in the field include Aleksander Jablonski, Gregorio Weber, and Andreas Albrecht. The principles of fluorescence polarization and some applications of the method are presented in Lakowicz's book.
Method and apparatus for the non-invasive measurement of tissue function and metabolism by determination of steady-state fluorescence anisotropy
InactiveUS8129105B2Safe high-resolution calibration-free measurementNon-invasive determination of the functional and metabolic status of tissuesMicrobiological testing/measurementMaterial analysis by optical meansSteady state fluorescenceNon invasive
A non-invasive measurement of biological tissue reveals information about the function of that tissue. Polarized light is directed onto the tissue, stimulating the emission of fluorescence, due to one or more endogenous fluorophors in the tissue. Fluorescence anisotropy is then calculated. Such measurements of fluorescence anisotropy are then used to assess the functional status of the tissue, and to identify the existence and severity of disease states. Such assessment can be made by comparing a fluorescence anisotropy profile with a known profile of a control.
Owner:MOORING SYST
Method of detecting analyte-molecule interactions
The invention provides methods for detecting an interaction between an analyte and a biomolecule. The method comprises separating at least one biomolecule according to its isoelectric point in the presence of a given analyte and detecting an interaction between the analyte and a biomolecule using fluorescence anisotropy. The method may further comprise collecting the analyte-biomolecule complex and analyzing the biomolecule.
Owner:SOUTHERN ILLINOIS UNIVERSITY
High-accuracy fluorescence anisotropy microscopic imaging device and method
The invention relates to a high-accuracy fluorescence anisotropy microscopic imaging device and method. The imaging method comprises the following steps of: on a reflective fluorescence microscope, leading lights emitted by a light source to pass through a polarizer, an excitation filter plate and a spectroscope, and then focusing the lights on a sample; leading fluorescence reflected by the sample to pass through an objective lens, the spectroscope and an emission filter plate, and then imaging on an image plane of a CCD (Charge-Coupled Device) camera through an adjustable angle analyzer; controlling the adjustable angle analyzer to rotate around an optical axis of an imaging optical path step by step by using a computer, wherein when the adjustable angle analyzer rotates by an angle every time, the CCD camera shoots a frame of polarized fluorescence image, M images are totally shot; and finally calculating fluorescence anisotropic images from the M images by using Fourier transform.The imaging method is characterized in that the measuring resolution ratio of the fluorescence anisotropy is greatly improved by establishing a single-channel fluorescence analyzing optical path; andthe microscopic imaging system has higher stability and better anti-jamming capability.
Owner:GUANGDONG UNIV OF TECH
Method and apparatus for the non-invasive measurement of tissue function and metabolism by determination of steady-state fluorescence anisotropy
ActiveUS20070243521A1Safe high-resolution calibration-free measurementNon-invasive determinationMicrobiological testing/measurementMaterial analysis by optical meansDiseaseSteady state fluorescence
A non-invasive measurement of biological tissue reveals information about the function of that tissue. Polarized light is directed onto the tissue, stimulating the emission of fluorescence, due to one or more endogenous fluorophors in the tissue. Fluorescence anisotropy is then calculated. Such measurements of fluorescence anisotropy are then used to assess the functional status of the tissue, and to identify the existence and severity of disease states. Such assessment can be made by comparing a fluorescence anisotropy profile with a known profile of a control.
Owner:MOORING SYST
Nanoscale imaging of molecular positions and anisotropies
InactiveUS20120018651A1High resolutionImprove spatial resolutionRaman/scattering spectroscopyPhotometryBeam splitterLight beam
A Polarization Fluorescence Photoactivation Localization Microscopy (P-FPALM) system and method are provided to simultaneously image the localizations and fluorescence anisotropics of large numbers of single molecules within a sample. The system modifies known FPALM systems by adding a polarizing beam splitter. The beam splitter polarizes emissions perpendicular and parallel to an axis in the sample to allow spatially separate imaging of fluorescence emitted from a sample. The system includes lenses and mirrors so that the separate, polarized beams are detected simultaneously. The present invention includes methods of using the system to image localizations and fluorescence anisotropics of single molecules, and methods of using data obtained with the system to predict 3-D orientation of the molecules. The system and method achieve substantially improved lateral resolution within even dense samples over known microscopic imaging techniques, and does not compromise speed or sensitivity.
Owner:UNIVERSITY OF MAINE
Method for detecting ochracin A based on fluorescence anisotropy of nucleic acid aptamer
The invention provides a method for detecting ochracin A based on the fluorescence anisotropy (fluorescence polarization) of a nucleic acid aptamer marked by a fluorescent dye tetramethylrhodamine (TMR for short). According to the method, the TMR is modified to a specific position of the aptamer of the ochracin A, the fluorescence anisotropy (fluorescence polarization) value of the TMR-marked nucleic acid aptamer is obviously changed due to the combination of the ochracin A, and the fluorescence anisotropy (fluorescence polarization) value is detected, so as to realize the quantitative detection of the ochracin A. The method has the advantages of simplicity and rapidness in operation, easiness in high throughput test, good specificity, high sensitivity and low cost.
Owner:SHANXI UNIV
Fluorescence polarization assay
InactiveUS20040077100A1Bioreactor/fermenter combinationsBiological substance pretreatmentsAnalyteFluorescent polarization assay
The present invention relates to methods for detecting the presence of one or more analytes of interest in a sample by measuring changes in fluorescence anisotropy as a result of binding of the analytes to specific aptamers. The aptamers are immobilized on a solid support and may be in the form of an array.
Owner:APPL BIOSYSTEMS INC
Dual-channel and single light path structure fluorescent anisotropy microscopic imaging device and method
InactiveCN102967554ACalculation accuracy has no effectSimple structureFluorescence/phosphorescenceMicroscopic imageTime-sharing
The invention relates to a dual-channel and single light path structure fluorescent anisotropic microscopic imaging device and a fluorescent anisotropic microscopic imaging method, which are suitable for transmission and reflection type microscopes. In the fluorescent anisotropic microscopic imaging device, a twisted nematic liquid crystal polarization rotator and a polarization analyzer are positioned on a fluorescence imaging light path, and the rotation center of the twisted nematic liquid crystal polarization rotator is coincident with the center of an optical axis of the fluorescence imaging light path, and the polarization direction of a polarizer, the polarization detection direction of the polarization analyzer, and the liquid crystal molecule arrangement direction on one side of incident light of the twisted nematic liquid crystal polarization rotator are parallel. According to the fluorescent anisotropic microscopic imaging method provided by the invention, the time-sharing control twisted nematic liquid crystal polarization rotator outputs a polarization state of light by switching high and low level signals, two tested sample fluorescence images, which are mutually perpendicular to the polarization direction, are correspondingly shot by a digital camera, and finally, fluorescent anisotropic microscopic images of the tested samples are calculated. On the premise of enhancing the fluorescent anisotropic imaging precision, the fluorescent anisotropic microscopic imaging device and the fluorescent anisotropic microscopic imaging method can fully utilize the view field of the digital camera, simplify the system structure, and make the cost lower.
Owner:GUANGDONG UNIV OF TECH
High temperature resistant non-fluorescence anti-sloughing nano-filtrate reducer and preparation method thereof
The invention discloses a high temperature resistant non-fluorescence anti-sloughing nano-filtrate reducer and a preparation method of the high temperature resistant non-fluorescence anti-sloughing nano-filtrate reducer. The anti-sloughing nano-filtrate reducer is synthesized of at least one non-water-soluble monomer and at least one water-soluble monomer with amino or sulfonic acid group by using an emulsion polymerization method in a non-oxygen environment under the condition of stirring. The nanoscale of the anti-sloughing nano-filtrate reducer is 10nm-5,000nm, the surface of the anti-sloughing nano-filtrate reducer contains the amino group with an inhibition function or sulfonic acid base or sulfonic acid group with strong temperature resistance, and the anti-sloughing nano-filtrate reducer has a core-shell structure. The reduction rate of shale swelling inhibition can be more than 70%, the reduction rate of medium pressure filtration can be more than 50%, and the rheological properties of drilling fluid are not significantly affected. After the drilling fluid containing the prepared anti-sloughing filtrate reducer is subjected to hot rolling at 220 DEG C, the performance of the drilling fluid is not significantly changed, so that the anti-sloughing filtrate reducer has a good high temperature resistant effect. The anti-sloughing filtrate reducer has very good application prospect in the term of anti-sloughing inhibition.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Method for measuring polarization degree and polarization angle of fluorescence spectrum
InactiveCN104048922AAccurate degree of polarizationPolarisation-affecting propertiesFluorescence spectrometrySpectrograph
The invention discloses a method for measuring a polarization degree and a polarization angle of a fluorescence spectrum. The method is used for solving the problems of different measurement polarization angles and errors changing over the fluorescence polarization angles during conventional fluorescence anisotropy measurement. The method is characterized by comprising the steps of regulating a polarization direction of a polarization detection polarizer to be parallel with a polarization direction of an emergent ray (completely polarized light) of a polarizer by adopting a common L measurement manner in fluorescence measurement through rotating the polarization detection polarizer, then clockwise rotating the polarization detection polarizer for 0 DEG, 45 DEG, 90 DEG and 135 DEG, respectively measuring by using a spectrograph and recording the fluorescence spectrum intensity, and calculating the polarization degree and the polarization angle by using the obtained fluorescence spectrum intensity. Compared with the prior art, the method has the beneficial effects that the polarization degree and the polarization angle of the fluorescence spectrum can be simultaneously obtained, and the polarization degree is more accurate.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Method of detecting analyte-biomolecule interactions
Owner:SOUTHERN ILLINOIS UNIVERSITY
Kit for detecting ATP with nucleic acid aptamer and detection method thereof
ActiveCN106932577AIncrease manufacturing costImprove stabilityMaterial analysisNucleic acid detectionSimple sample
The invention provides a kit for detecting an ATP with a nucleic acid aptamer and a detection method thereof. The kit comprises a nucleic acid aptamer having a sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2, and an ATP labeled by Tetra-Methyl Rhodamine (TMR). The kit achieves detection of the ATP based on fluorescence polarization (fluorescence anisotropy) of the nucleic acid aptamer, and has the advantages of simple operation, fast detection, high sensitivity, high accuracy and good reproducibility, and is insusceptible to fluorescence intensity fluctuation and fluorescence photobleaching. Compared with such methods as chromatography, no expensive instrument is needed and the operation steps are simple; and moreover, determination can be achieved just by simple sample mixing.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Apparatus for the non-invasive measurement of tissue function and metabolism by determination of steady-state fluorescence anisotropy
ActiveUS20080254531A1Safe high-resolution calibration-free measurementNon-invasive determination of the functional and metabolic status of tissuesBioreactor/fermenter combinationsBiological substance pretreatmentsSteady state fluorescenceMedicine
A non-invasive measurement of biological tissue reveals information about the function of that tissue. Polarized light is directed onto the tissue, stimulating the emission of fluorescence, due to one or more endogenous fluorophors in the tissue. Fluorescence anisotropy is then calculated. Such measurements of fluorescence anisotropy are then used to assess the functional status of the tissue, and to identify the existence and severity of disease states. Such assessment can be made by comparing a fluorescence anisotropy profile with a known profile of a control.
Owner:MOORING SYST
Method for analyzing aflatoxin B1 by fluorescence anisotropy of sensitive aptamer
ActiveCN110095442AEasy to introduceReduce dosageFluorescence/phosphorescenceDNA/RNA fragmentationAptamerBiotin-streptavidin complex
The invention discloses a method for analyzing aflatoxin B1 by fluorescence anisotropy of a sensitive aptamer. The method protected by the invention comprises the steps of: subjecting the aptamer marked by means of a fluorochrome, single-chain DNA molecules marked by means of streptavidin and a to-be-tested sample to co-reaction, and realizing detection of aflatoxin B1 in the to-be-tested sample through determining a fluorescence anisotropic value of a reaction system, wherein the aptamer can be specifically bound to the aflatoxin B1, and the single-chain DNA molecules can form a double-chainstructure with the aptamer through reverse complementation; and placing the fluorochrome and the streptavidin on the same end of the double-chain structure after the single-chain DNA molecules form the double-chain structure with the aptamer through reverse complementation, wherein the fluorochrome and the streptavidin are closed to each other spatially. The method has the advantages of being sensitive, simple and rapid, having good repeatability, being easy in high-throughput analysis, having low sample consumption and the like, the materials used are easy to prepare, the synthesis cost is low, the stability is good, the storage and transportation are convenient, the life of the shelf is long, and the method has high sensitivity and good selectivity.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Sulfion sensor based on polyethylene glycol and preparation method and application thereof
ActiveCN104927045AReduce cost inputHigh sensitivityFluorescence/phosphorescenceLuminescent compositionsSolubilityPolyethylene glycol
The invention discloses a sulfion sensor based on polyethylene glycol and a preparation method and application thereof. A fluorescence sensor is prepared by raw materials such as monoamino group polyethylene glycol, 4-bromine-1, 8-anhydride naphthalene and sodium azide, and the fluorescence sensor can identify a sulfion with high sensitivity and high selectivity in the water. According to the sensor, the sulfion is identified selectively through the theory of intramolecular charge transfer (ICT). Due to the fact that sulfion concentration in a system increases gradually, the reducibility of the sulfion can give rise to the generation of the ICT process in the sensor, the fluorescence of the sensor is enhanced gradually, and the absorption strength changes correspondingly. Compared with an existing fluorescence detection technology, the fluorescence sensor is great in water solubility, the detection to the sulfion is high in sensitivity and good in selectivity, the synthetic route is simple, the detection equipment and method are simple and convenient, the sensor is applicable to magnify synthesis and practical production application, and the sensor has a vast application prospect in technical fields such as analytical chemistry, life sciences and environmental sciences.
Owner:HUNAN UNIV OF SCI & TECH
System and method for carrying out polarization super-resolution imaging on fluorescence anisotropy
ActiveCN113466187AHigh image resolutionDouble the spatial resolutionFluorescence/phosphorescenceImage resolutionDeconvolution
The invention relates to a system and method for carrying out polarization super-resolution imaging on fluorescence anisotropy. According to the system, an optical phase-locked detection technology and a linear dichroic system are used to obtain a polarization modulation image sequence. The method comprises the following steps of: S1, carrying out frequency domain phase-locked processing on an acquired fluorescence image sequence modulated by rotating polarized light, extracting signals with the same modulation frequency, and obtaining a sequence image IOLID which only retains background and modulation frequency information; and S2, performing demodulation, namely three-dimensional deconvolution, on the sequence image IOLID to obtain a super-resolution ac image G'ac, a reconstructed dc image G'dc and a super-resolution reconstructed image G 'at the same time, and performing phase extraction to obtain a dipole orientation distribution image. The OLID-SDOM disclosed by the invention reveals fluorescence anisotropy which is never observed before in part of samples, which shows that the OLID-SDOM has potential to observe dynamic molecular structures; and compared with a traditional microscope, the OLID-SDOM has the advantages that the spatial resolution is doubled, and biological samples are not limited during imaging.
Owner:TSINGHUA UNIV
Method for detecting ochratoxin A through fluorescence anisotropy technology
ActiveCN110117641AReduce dosageSolution to short lifeMicrobiological testing/measurementBiotin-streptavidin complexAptamer
The invention discloses a method for detecting ochratoxin A through a fluorescence anisotropy technology. According to the method, an aptamer capable of being specifically combined with the ochratoxinA is selected and marked with a fluorochrome; a single-stranded DNA molecule which is complementary to the aptamer is designed for the aptamer, and marked with streptavidin; after the aptamer is combined with the single-stranded DNA molecule, the fluorochrome and the streptavidin are adjacent to each other in space; the prepared aptamer and the prepared single-stranded DNA molecule react with a to-be-tested sample, and by measuring the fluorescence anisotropy value of the reaction system, the ochratoxin A in the to-be-tested sample is detected. The built method has the advantages that the method is sensitive, simple, quick and good in reproducibility, high-throughput analysis is easy to achieve, and the use quantity of the sample is small; the adopted materials are easy to prepare, the synthesis cost is low, the stability is high, storage and transportation are convenient, the shelf life is long, and the sensitivity and selectivity are high.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Fluorescent dye labeling aptamer for immune globulin E with sensitive fluorescence anisotropy response
ActiveCN107868787AHigh affinitySignificant fluorescence polarization (fluorescence anisotropy) changesBiological testingDNA/RNA fragmentationFluoresceinGlobulin
The invention discloses a fluorescent dye labeling aptamer for immune globulin E with sensitive fluorescence anisotropy (fluorescence polarization) response. The aptamer disclosed by the invention canbe specifically bound to immune globulin E (IgE) so as to produce an obvious fluorescence anisotropy (fluorescence polarization) signal change. A fluorescent dye fluorescein or tetramethyl rhodamineis modified to base sites of specific nucleotides of the aptamer of the IgE, so that the obtained fluorescent dye labeling aptamer maintains high affinity and is capable of producing sensitive signalresponse to the IgE. The aptamer labeled by the fluorescent dyes is capable of sensitively and rapidly detecting the IgE. The fluorescence anisotropy (fluorescence polarization) detection method is simple in operation and excellent in reproducibility.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Fluorescence polarization assay
InactiveUS20070054308A1Bioreactor/fermenter combinationsBiological substance pretreatmentsAnalyteFluorescent polarization assay
The present invention relates to methods for detecting the presence of one or more analytes of interest in a sample by measuring changes in fluorescence anisotropy as a result of binding of the analytes to specific aptamers. The aptamers are immobilized on a solid support and may be in the form of an array.
Owner:APPL BIOSYSTEMS INC
A quick way to identify ag + Molecular logic gate with three-phase output function of and cys and its construction method
ActiveCN111122523BImprove accuracyHigh sensitivityBiomolecular computersFluorescence/phosphorescenceAptamerBerberine
A rapid identification of Ag + The molecular logic gate and the construction method thereof of the three-phase output function of Cys belong to the field of molecular computer technology, which can solve the limitation problem of the single signal output of the existing molecular logic gate, including: using Cys and Ag + As two parallel input signals, the output signals are fluorescence intensity, fluorescence anisotropy and fluorescence lifetime. With berberine as the signal reporting unit, Ag + ‑Nucleic acid aptamer is a molecular recognition substrate, using berberine in Ag + ‑Aptamer and Cys, Ag + The changes of fluorescence intensity, fluorescence anisotropy and fluorescence attenuation in different microenvironments when it exists, construct the basic Boolean logic gate of "forbidden" gate. The constructed molecular logic gate circuit has good performance, high sensitivity, and is easy to distinguish, and can solve the limitation brought by a single output signal.
Owner:SHANXI UNIV
Blue light polarizing thin film and preparation method thereof as well as blue light backlight source and liquid crystal display device
InactiveCN104327845APolarizedPolarising elementsLuminescent compositionsLiquid-crystal displayFluorescence spectrometry
The invention discloses a blue light polarizing thin film and a preparation method thereof as well as a blue light backlight source and a liquid crystal display device. The blue light polarizing thin film disclosed by the invention is a multi-layer thin film formed by virtue of layer-by-layer deposition of magnesium-aluminum hydrotalcite and tri(8-hydroxyquinoline-5-sulfonic acid) aluminum. The maximum emission value of fluorescence spectrum of the blue light polarizing thin film disclosed by the invention is 470-490nm; within a blue light area and fractional anisotropy of fluorescence is 0.1-0.2, the thin film has a polarizing characteristic and is applicable to the backlight source of a liquid crystal display.
Owner:BOE TECH GRP CO LTD +1
A kit for detecting ATP with nucleic acid aptamer and its detection method
ActiveCN106932577BIncrease manufacturing costImprove stabilityMaterial analysisDNA/RNA fragmentationAptamerSimple sample
The invention provides a kit for detecting an ATP with a nucleic acid aptamer and a detection method thereof. The kit comprises a nucleic acid aptamer having a sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2, and an ATP labeled by Tetra-Methyl Rhodamine (TMR). The kit achieves detection of the ATP based on fluorescence polarization (fluorescence anisotropy) of the nucleic acid aptamer, and has the advantages of simple operation, fast detection, high sensitivity, high accuracy and good reproducibility, and is insusceptible to fluorescence intensity fluctuation and fluorescence photobleaching. Compared with such methods as chromatography, no expensive instrument is needed and the operation steps are simple; and moreover, determination can be achieved just by simple sample mixing.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Fluorescence anisotropic microscopic imaging device and method with dual-channel and single-light path structure
InactiveCN102967554BCalculation accuracy has no effectSimple structureFluorescence/phosphorescenceMicroscopic imageMicro imaging
The invention relates to a dual-channel and single light path structure fluorescent anisotropic microscopic imaging device and a fluorescent anisotropic microscopic imaging method, which are suitable for transmission and reflection type microscopes. In the fluorescent anisotropic microscopic imaging device, a twisted nematic liquid crystal polarization rotator and a polarization analyzer are positioned on a fluorescence imaging light path, and the rotation center of the twisted nematic liquid crystal polarization rotator is coincident with the center of an optical axis of the fluorescence imaging light path, and the polarization direction of a polarizer, the polarization detection direction of the polarization analyzer, and the liquid crystal molecule arrangement direction on one side of incident light of the twisted nematic liquid crystal polarization rotator are parallel. According to the fluorescent anisotropic microscopic imaging method provided by the invention, the time-sharing control twisted nematic liquid crystal polarization rotator outputs a polarization state of light by switching high and low level signals, two tested sample fluorescence images, which are mutually perpendicular to the polarization direction, are correspondingly shot by a digital camera, and finally, fluorescent anisotropic microscopic images of the tested samples are calculated. On the premise of enhancing the fluorescent anisotropic imaging precision, the fluorescent anisotropic microscopic imaging device and the fluorescent anisotropic microscopic imaging method can fully utilize the view field of the digital camera, simplify the system structure, and make the cost lower.
Owner:GUANGDONG UNIV OF TECH
Preparation method of fluorescence antibody for detecting Newcastle disease virus and solid-phase immunofluorescence detection assay kit
InactiveCN101975856BHigh sensitivityReduce distractionsSerum immunoglobulinsImmunoglobulins against virusesCross-linkMicrosphere
Owner:QILU UNIV OF TECH
Blue light polarizing film, preparation method thereof, blue light backlight source and liquid crystal display device
InactiveCN104327845BPolarizedPolarising elementsLuminescent compositionsLiquid-crystal displayFluorescence spectrometry
The invention discloses a blue light polarizing film, a preparation method thereof, a blue light backlight source and a liquid crystal display device. The blue light polarizing film of the present invention is a multilayer film formed by alternate deposition of magnesium aluminum hydrotalcite and tris(8-hydroxyquinoline-5-sulfonic acid)aluminum. The maximum emission value of the fluorescence spectrum of the blue light polarizing film of the present invention is 470-490nm, which is in the blue light region, and the fluorescence anisotropy value is 0.1-0.2, has polarization characteristics, and can be used for liquid crystal display backlight.
Owner:BOE TECH GRP CO LTD +1
A method for detecting ochratoxin a using fluorescence anisotropy technique
ActiveCN110117641BReduce dosageSolution to short lifeMicrobiological testing/measurementAptamerSingle strand
The invention discloses a method for detecting ochratoxin A through a fluorescence anisotropy technology. According to the method, an aptamer capable of being specifically combined with the ochratoxinA is selected and marked with a fluorochrome; a single-stranded DNA molecule which is complementary to the aptamer is designed for the aptamer, and marked with streptavidin; after the aptamer is combined with the single-stranded DNA molecule, the fluorochrome and the streptavidin are adjacent to each other in space; the prepared aptamer and the prepared single-stranded DNA molecule react with a to-be-tested sample, and by measuring the fluorescence anisotropy value of the reaction system, the ochratoxin A in the to-be-tested sample is detected. The built method has the advantages that the method is sensitive, simple, quick and good in reproducibility, high-throughput analysis is easy to achieve, and the use quantity of the sample is small; the adopted materials are easy to prepare, the synthesis cost is low, the stability is high, storage and transportation are convenient, the shelf life is long, and the sensitivity and selectivity are high.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Controlled doping of anodic aluminum oxide for enhanced fluorescence and methods of preparation
PendingUS20210262939A1Controlled level of fluorescence enhancementSafe working environmentAnodisationSemi-permeable membranesDrug discoveryFörster resonance energy transfer
Nano-structured anodic aluminum oxide ceramic films and membranes doped with chelated metals for fluorescence enhancement. Controlled doping during production steps results in the inclusion of traces of ions in the finished materials while maintaining high control over the film structure. This approach yields products suitable for optical applications, including fluorescence enhancement. The nano-structured anodic aluminum oxide ceramic films and membranes are particularly useful for in vitro diagnostics, drug discovery, DNA sequencing, proteomics, immunofluorescence, immunohistochemistry, biosensing, and bio-assay fluorescence technologies such as time resolved Forster resonance energy transfer (TR-FRET), Fluorescence in situ hybridization (FISH), Fluorescence-lifetime imaging microscopy (FLIM), Fluorescence polarization immunoassay (FPIA), Fluorescence anisotropy or fluorescence polarization, Fluorescence recovery after photobleaching (FRAP), Fluorescence Loss in Photobleaching (FLIP), Fluorescence correlation spectroscopy (FCS), and Falck-Hillarp fluorescence (F-H).
Owner:NANOPEC INC
Molecular logic gate with three-phase output function for quickly identifying Ag<+> and Cys, and construction method of molecular logic gate
ActiveCN111122523AImprove accuracyHigh sensitivityBiomolecular computersFluorescence/phosphorescenceAptamerBerberine
The invention discloses a molecular logic gate with a three-phase output function for quickly identifying Ag<+> and Cys and a construction method of the molecular logic gate, which belong to the technical field of molecular computers, and can solve the problem of limitation of single signal output of an existing molecular logic gate. The molecular logic gate is characterized in that: Cys and Ag<+>are taken as two parallel input signals, and fluorescence intensity, fluorescence anisotropy and fluorescence lifetime are taken as output signals; and an ''inhibit '' gate, namely, a basic Boolean logic gate is constructed by taking berberine as a signal report unit and an Ag<+>-aptamer as a molecular recognition matrix and utilizing changes of fluorescence intensity, fluorescence anisotropy andfluorescence attenuation of the berberine in different microenvironments in the presence of the Ag<+>-aptamer, Cys and Ag<+>. The constructed molecular logic gate circuit is good in performance, highin sensitivity and easy to distinguish, and can solve the limitation caused by a single output signal.
Owner:SHANXI UNIV
Application of amentoflavone in preparation of staphylococcus aureus MgrA inhibitor
InactiveCN112826818AReduce adhesionInhibitory activityAntibacterial agentsOrganic active ingredientsStaphyloccocus aureusDrug target
The invention relates to an application of amentoflavone in preparation of a staphylococcus aureus transcriptional adjustment factor (MgrA) inhibitor, the staphylococcus aureus transcriptional adjustment factor (MgrA) is taken as a drug target, and the drug amentoflavone capable of remarkably inhibiting the activity of the MgrA is screened from natural compounds by using a fluorescence anisotropy analysis method. The toxicity of the methicillin-resistant staphylococcus aureus can be obviously reduced on the premise of not influencing the normal growth of bacteria, and the generation of bacterial drug resistance is not easily caused. In in-vivo experiments, amentoflavone has a remarkable treatment effect on mouse pneumonia caused by methicillin-resistant staphylococcus aureus infection, and can greatly improve the viability of infected mice and relieve the inflammatory response of lung tissues. In conclusion, the amentoflavone serves as an effective staphylococcus aureus transcriptional adjustment factor MgrA inhibitor, and a new research and development strategy and a lead compound are provided for effectively preventing and treating methicillin-resistant staphylococcus aureus infection clinically.
Owner:JILIN UNIV
Quantitative measurement method for fluorescence lifetime and fluorescence dynamic anisotropic parameters
InactiveCN102680438BQuantitative measurement of fluorescence lifetimeFluorescence/phosphorescencePhotovoltaic detectorsRotational correlation time
The invention relates to a measuring method for fluorescence characteristics of a fluorescence product and particularly relates to a method for quantitative measurement method for fluorescence lifetime and fluorescence dynamic anisotropic parameters. The method comprises the steps of: exciting a product by a pulse or sine polarization exciting light to produce fluorescence; detecting intensity of polarized fluorescence which is parallel to the pulse or sine polarization exciting light and intensity of polarized fluorescence which is perpendicular to the pulse or sine polarization exciting light respectively with a photoelectric detector; and calculating the average life, the fluorescence anisotropy at the initial time, the fluorescence anisotropy at the infinite time and fluorescence molecule rotational correlation time of the sample. With the adoption of the method provided by the invention, the fluorescence lifetime of all samples can be effectively and quantitatively measured; moreover, the invention provides a quantitative measurement method for fluorescence parameters of the fluorescence product, so the method has great application value for quantitative study of the interaction between large biological molecules, such as proteins, in the living cell.
Owner:GUANGDONG UNIV OF TECH
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