34 results about "Fluorescence anisotropy" patented technology

The invention provides methods for detecting an interaction between an analyte and a biomolecule. The method comprises separating at least one biomolecule according to its isoelectric point in the presence of a given analyte and detecting an interaction between the analyte and a biomolecule using fluorescence anisotropy. The method may further comprise collecting the analyte-biomolecule complex and analyzing the biomolecule.

The present invention relates to methods for detecting the presence of one or more analytes of interest in a sample by measuring changes in fluorescence anisotropy as a result of binding of the analytes to specific aptamers. The aptamers are immobilized on a solid support and may be in the form of an array.

The invention provides a kit for detecting an ATP with a nucleic acid aptamer and a detection method thereof. The kit comprises a nucleic acid aptamer having a sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2, and an ATP labeled by Tetra-Methyl Rhodamine (TMR). The kit achieves detection of the ATP based on fluorescence polarization (fluorescence anisotropy) of the nucleic acid aptamer, and has the advantages of simple operation, fast detection, high sensitivity, high accuracy and good reproducibility, and is insusceptible to fluorescence intensity fluctuation and fluorescence photobleaching. Compared with such methods as chromatography, no expensive instrument is needed and the operation steps are simple; and moreover, determination can be achieved just by simple sample mixing.

The invention relates to a preparation method of fluorescence antibody for detecting a Newcastle disease virus and a solid-phase immunofluorescence detection assay kit. The preparation method comprises the following steps of: respectively deriving R-phycoerythrin (RPE) and an antibody resisting the Newcastle disease virus (NDV) by using a cross-linking agent SPDP (N-succinimidyl-3-(2-pyridyldithiol) propionate), cross-linking the derivatives in a proper molar ratio, and purifying through HPLC (High Performance Liquid Chromatography) to prepare an RPE marked NDV fluorescence antibody. The solid-phase immunofluorescence detection assay kit is formed from the fluorescence antibody, an NDV-resisting antibody, an agarose microsphere, diluted hydrochloric acid and a washing liquid. The assay kit comprises the following detection flows of: coating an activated microsphere with the antibody, washing, combining with a sample to be measured, washing, combining with the fluorescence antibody, fully washing, exciting by blue and green light in a fluorescence microscope, observing and judging the result. The prepared fluorescence antibody has the advantages of high yield, high purity, bright orange fluorescence and good stability; and the assay kit has signal enrichment action by using a spherical carrier and can increase detection sensitivity. The invention is applicable to the rapid detection of the Newcastle disease virus.

The invention discloses a method for detecting ochratoxin A through a fluorescence anisotropy technology. According to the method, an aptamer capable of being specifically combined with the ochratoxinA is selected and marked with a fluorochrome; a single-stranded DNA molecule which is complementary to the aptamer is designed for the aptamer, and marked with streptavidin; after the aptamer is combined with the single-stranded DNA molecule, the fluorochrome and the streptavidin are adjacent to each other in space; the prepared aptamer and the prepared single-stranded DNA molecule react with a to-be-tested sample, and by measuring the fluorescence anisotropy value of the reaction system, the ochratoxin A in the to-be-tested sample is detected. The built method has the advantages that the method is sensitive, simple, quick and good in reproducibility, high-throughput analysis is easy to achieve, and the use quantity of the sample is small; the adopted materials are easy to prepare, the synthesis cost is low, the stability is high, storage and transportation are convenient, the shelf life is long, and the sensitivity and selectivity are high.