A method for detecting ochratoxin a using fluorescence anisotropy technique

An ochratoxin and anisotropic technology, applied in the field of detection of ochratoxin A, can solve the problems of high antibody preparation cost, poor antibody stability, cumbersome and time-consuming operation, etc., and achieve easy label introduction and high-throughput analysis , convenient storage and transportation
CN110117641BActive Publication Date: 2021-02-09RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Publication Date
2021-02-09

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Abstract

The invention discloses a method for detecting ochratoxin A through a fluorescence anisotropy technology. According to the method, an aptamer capable of being specifically combined with the ochratoxinA is selected and marked with a fluorochrome; a single-stranded DNA molecule which is complementary to the aptamer is designed for the aptamer, and marked with streptavidin; after the aptamer is combined with the single-stranded DNA molecule, the fluorochrome and the streptavidin are adjacent to each other in space; the prepared aptamer and the prepared single-stranded DNA molecule react with a to-be-tested sample, and by measuring the fluorescence anisotropy value of the reaction system, the ochratoxin A in the to-be-tested sample is detected. The built method has the advantages that the method is sensitive, simple, quick and good in reproducibility, high-throughput analysis is easy to achieve, and the use quantity of the sample is small; the adopted materials are easy to prepare, the synthesis cost is low, the stability is high, storage and transportation are convenient, the shelf life is long, and the sensitivity and selectivity are high.
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Description

technical field

[0001] The invention relates to a method for detecting ochratoxin A by using fluorescence anisotropy technology. Background technique

[0002] Ochratoxin A (ochratoxin A, abbreviated as OTA) is a secondary metabolite produced by several Penicillium fungi such as Ochratoxin, which is listed as a 2B carcinogen by the International Agency for Research on Cancer. Cereals (wheat, corn, etc.), grains, grapes, coffee, etc. are often contaminated by OTA. Ingestion of OTA-contaminated food poses a serious threat to animal and human health. Sensitive and rapid detection of OTA is of great significance and demand in food safety, quality control, environmental analysis, especially on-site analysis.

[0003] Commonly used methods for detecting ochratoxin A mainly include chromatography, chromatography-mass spectrometry, mass spectrometry, and immunoassay sensing. Chromatography and mass spectrometry often require expensive instruments and equipment, and the operation i...

Claims

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