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5 results about "Fluorescence correlation spectroscopy" patented technology
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Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity. The analysis provides parameters of the physics under the fluctuations. One of the interesting applications of this is an analysis of the concentration fluctuations of fluorescent particles (molecules) in solution. In this application, the fluorescence emitted from a very tiny space in solution containing a small number of fluorescent particles (molecules) is observed. The fluorescence intensity is fluctuating due to Brownian motion of the particles. In other words, the number of the particles in the sub-space defined by the optical system is randomly changing around the average number. The analysis gives the average number of fluorescent particles and average diffusion time, when the particle is passing through the space. Eventually, both the concentration and size of the particle (molecule) are determined. Both parameters are important in biochemical research, biophysics, and chemistry.
Multichannel fluorescence correlation spectrum multimer detection data processing method
The invention discloses a multi-channel fluorescence correlation spectrum polymer detection data processing method, and relates to the technical field of biological detection and spectral analysis, and the method comprises the following steps: according to a calibrated data set, calculating and correcting the light path difference of each pair of channels one by one, and filtering pseudo correlation signals at the same time to obtain a time stamp data set after noise filtering; marking the target polymer molecule as a donor probe and an acceptor probe according to the timestamp data set after noise filtering, and calculating the time correlation between the donor and the acceptor to obtain a timestamp pair set of the FRET event; and performing FCS analysis on the timestamp pair set of the FRET event, calculating diffusion time, comparing the diffusion time with known monomer diffusion time, judging whether the FRET event is a real polymer signal or not, and outputting a polymer event detection result set. According to the invention, by adjusting the physical optical path difference between different channels, the time deviation caused by the physical distance is effectively reduced.
Owner:HANGZHOU BOHENG TECH CO LTD
A method and apparatus for fast random addressing of scanning fluorescence correlation spectroscopy
The application provides a fast random addressing scanning fluorescence correlation spectroscopy method and device thereof, comprising the following steps: S1, using a surface array camera to collect a series of wide field images under light sheet illumination, and obtaining a global low time resolution image fluorescence correlation spectroscopy diffusion image by calculation; S2, selecting an interested region according to the wide field fluorescence correlation spectroscopy image and setting a scanning array generation condition; S3, automatically generating a scanning array according to the selected arbitrary interested region and the set condition.The fast random addressing scanning fluorescence correlation spectroscopy method and device thereof provided by the application can solve the problem that the traditional scanning FCS cannot detect fast kinetics due to slow scanning speed, and can also solve the problem that the traditional scanning FCS cannot simultaneously monitor multiple regions due to the mechanical scanning of the scanning galvanometer.
Owner:SOUTH CHINA NORMAL UNIV
A data processing method for polymer detection using multi-channel fluorescence correlation spectroscopy
The present invention discloses a multi-channel fluorescence correlation spectroscopy (FCS) polymer detection data processing method, which relates to the field of biological detection and spectral analysis technology. The method comprises the following steps: calculating the optical path difference of each pair of channels one by one and correcting it based on a calibrated data set; filtering out pseudo-correlation signals; obtaining a noise-filtered timestamp data set; labeling the target polymer molecules as donor probes and acceptor probes based on the noise-filtered timestamp data set; calculating the temporal correlation between the donor and the acceptor; obtaining a timestamp pair set of FRET events; performing FCS analysis on the timestamp pair set of FRET events, calculating the diffusion time and comparing it with the known monomer diffusion time, determining whether the FRET event is a true polymer signal, and outputting a set of polymer event detection results. The present invention effectively reduces the time deviation caused by physical distance by adjusting the physical optical path differences between different channels.
Owner:HANGZHOU BOHENG TECH CO LTD
Microscopy method, especially for fluorescence correlation spectroscopy
A microscopy method involves directing a focused beam of excitation radiation at an examination location of an object to be examined, to create an excitation volume in the object. An overview image is used to identify at least one structure of the object and define at least one of the identified structures as a reference structure. A spatial relationship is defined between the positions of the examination location and the reference structure. Detection radiation coming from the excitation volume is acquired as measurement values over an overall measurement duration, the overall measurement duration being subdivided into a plurality of measurement intervals. At least every second measurement interval is preceded by a comparison of the current position of the focused beam with a current position of the examination location. The positioning of the focused beam is corrected in the case of an inadmissible deviation of the current positions.
Owner:CARL ZEISS MICROSCOPY GMBH
Method for quantitatively analyzing fluorescent dyes labeled on extracellular vesicle by using fluorescence correlation spectroscopy, and use thereof
A method of quantifying fluorescent dyes labeled on extracellular vesicles, including providing an extracellular vesicle molecule labeled with a fluorescence dye molecule, and determining a brightness of each of the extracellular vesicle molecule labeled with the fluorescence dye molecule and the fluorescence dye molecule through fluorescence correlation spectroscopy (FCS) of the extracellular vesicle molecule and the fluorescence dye molecule.
Owner:THE ASAN FOUND +1