In-water polycyclic aromatic hydrocarbon detection method based on two-dimensional fluorescence correlation spectroscopy

Abstract

The invention discloses an in-water polycyclic aromatic hydrocarbon detection method based on two-dimensional fluorescence correlation spectroscopy.The method includes: (1) preparing mixed experimental polycyclic aromatic hydrocarbon aqueous solutions of different concentrations; (2) scanning fluorescence spectrums of each mixed experimental polycyclic aromatic hydrocarbon aqueous solution under different excitation wavelengths to obtain one-dimensional dynamic fluorescence spectrums; (3) adopting data of the obtained one-dimensional dynamic fluorescence spectrums to form a spectrum matrix, and carrying out two-dimensional correlation calculation to obtain a synchronous two-dimensional fluorescence correlation spectrum matrix; (4) establishing a quantitative analysis model according to the synchronous two-dimensional fluorescence correlation spectrum matrix and a concentration variable matrix of anthracene, phenanthrene and pyrene in each mixed polycyclic aromatic hydrocarbon aqueous solution; (5) substituting a synchronous two-dimensional fluorescence correlation spectrum of an unknown sample aqueous solution into the quantitative analysis model established at the step (4) to obtain concentrations of anthracene, phenanthrene and pyrene in the unknown sample aqueous solution.The in-water polycyclic aromatic hydrocarbon detection method based on two-dimensional fluorescence correlation spectroscopy has the advantages that feature information of polycyclic aromatic hydrocarbon pollutants can be extracted more effectively, quantitative analysis of in-water polycyclic aromatic hydrocarbon is realized, and easiness in operation and high analysis efficiency and analysis precision are achieved.

Description

technical field [0001] The invention belongs to the field of detection methods, in particular to a detection method for polycyclic aromatic hydrocarbons in water based on two-dimensional fluorescence correlation spectrum. Background technique [0002] Polycyclic aromatic hydrocarbons are hydrocarbon compounds in which two or more benzene rings are linked together. PAHs are ubiquitous in the environment, and many PAHs are carcinogenic, teratogenic, mutagenic, and bioaccumulative, and can remain in the environment for a long time. Almost all rivers and lakes in my country have been polluted by polycyclic aromatic hydrocarbons to varying degrees. To restore and treat polluted water bodies, it is first necessary to clarify the basic information such as the types and contents of polycyclic aromatic hydrocarbon pollutants in the water. Therefore, the research on the rapid detection method of PAH content in water is of great significance. [0003] At present, there have been man...

AI Technical Summary

Problems solved by technology

However, since the polycyclic aromatic hydrocarbons to be analyzed are mostly located in complex matrices, and there are many types and similar structures, the conventional fluorescence spectra (one-dimensional spectrum and excitation-emission matrix spectrum) overlap each other, and cannot analyze the polycyclic aromatic hydrocarbons in the environment. Effective extraction of characteristic fluorescence information of aromatic hydrocarbon pollutants

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