Method for measuring polarization degree and polarization angle of fluorescence spectrum

Abstract

The invention discloses a method for measuring a polarization degree and a polarization angle of a fluorescence spectrum. The method is used for solving the problems of different measurement polarization angles and errors changing over the fluorescence polarization angles during conventional fluorescence anisotropy measurement. The method is characterized by comprising the steps of regulating a polarization direction of a polarization detection polarizer to be parallel with a polarization direction of an emergent ray (completely polarized light) of a polarizer by adopting a common L measurement manner in fluorescence measurement through rotating the polarization detection polarizer, then clockwise rotating the polarization detection polarizer for 0 DEG, 45 DEG, 90 DEG and 135 DEG, respectively measuring by using a spectrograph and recording the fluorescence spectrum intensity, and calculating the polarization degree and the polarization angle by using the obtained fluorescence spectrum intensity. Compared with the prior art, the method has the beneficial effects that the polarization degree and the polarization angle of the fluorescence spectrum can be simultaneously obtained, and the polarization degree is more accurate.

Description

Technical field [0001] The invention relates to a polarization spectrum analysis technology, in particular to a method for measuring the polarization degree and the polarization angle of the fluorescence spectrum, which is suitable for spectrum biological material analysis and belongs to the technical field of spectrum analysis. Background technique [0002] Traditional polarization spectroscopy technology is to analyze the biological response by detecting the degree of polarization or anisotropy of the fluorescence spectrum. The polarization angle associated with the fluorescence mechanism and electronic structure of organisms or materials has not been measured. In the polarization measurement, the default fluorescence polarization direction is consistent with the polarization direction of the incident light, that is, the polarization angle is 0 degrees. The actual situation is that the polarization angle changes with the wavelength of the incident light, and its value is uncert...

AI Technical Summary

Problems solved by technology

The actual situation is that the polarization angle varies with the wavelength of the incident light, and its value is uncertain, so the traditional method of measuring the degree of polarization is inaccurate

Especially when the actual polarization angle of the fluorescence is 45 degrees, the value of the degree of polarization measured by the traditional measurement method is always 0, and its error rate reaches 100%.

[0003] In the existing literature, no measurement method for the polarization angle of fluorescence has been found, and the measurement of the degree of polarization has shortcomings such as inaccuracy

Images