Method for measuring polarization degree and polarization angle of fluorescence spectrum

A fluorescence spectrum and fluorescence polarization technology, applied in the field of spectral analysis, can solve the problems of inaccurate measurement of polarization degree, inaccurate measurement method of polarization degree, uncertain value, etc., and achieve the effect of accurate polarization degree

Inactive Publication Date: 2014-09-17
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF6 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The actual situation is that the polarization angle varies with the wavelength of the incident light, and its value is uncertain, so the traditional method of measuring the degree of polarization is inaccurate
Especially when the actual polarization angle of the fluorescence is 45 degrees, the value of the degr

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring polarization degree and polarization angle of fluorescence spectrum
  • Method for measuring polarization degree and polarization angle of fluorescence spectrum
  • Method for measuring polarization degree and polarization angle of fluorescence spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] According to the above technical solution, the present invention will be described in detail below with reference to the drawings and implementation examples.

[0038] In this embodiment, the polarization degree and the polarization angle of the olive oil are measured by the method for measuring the polarization degree and the polarization angle of the fluorescence spectrum provided by the present invention, and the specific steps are as follows:

[0039] Step 1: Place the laser light source 1, the polarizing polarizer 2, the cuvette 3, the analyzer 4 and the spectrometer 5 on the same horizontal plane P, such as figure 1 Shown. The cuvette 3 is filled with olive oil.

[0040] Step 2: The continuous laser light with a wavelength of 473 nanometers emitted by the laser light source 1 is incident on the polarizing polarizer 2, and the emitted light is completely polarized light, and the polarization direction is perpendicular to the horizontal plane P;

[0041] Step 3: The fully p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for measuring a polarization degree and a polarization angle of a fluorescence spectrum. The method is used for solving the problems of different measurement polarization angles and errors changing over the fluorescence polarization angles during conventional fluorescence anisotropy measurement. The method is characterized by comprising the steps of regulating a polarization direction of a polarization detection polarizer to be parallel with a polarization direction of an emergent ray (completely polarized light) of a polarizer by adopting a common L measurement manner in fluorescence measurement through rotating the polarization detection polarizer, then clockwise rotating the polarization detection polarizer for 0 DEG, 45 DEG, 90 DEG and 135 DEG, respectively measuring by using a spectrograph and recording the fluorescence spectrum intensity, and calculating the polarization degree and the polarization angle by using the obtained fluorescence spectrum intensity. Compared with the prior art, the method has the beneficial effects that the polarization degree and the polarization angle of the fluorescence spectrum can be simultaneously obtained, and the polarization degree is more accurate.

Description

Technical field [0001] The invention relates to a polarization spectrum analysis technology, in particular to a method for measuring the polarization degree and the polarization angle of the fluorescence spectrum, which is suitable for spectrum biological material analysis and belongs to the technical field of spectrum analysis. Background technique [0002] Traditional polarization spectroscopy technology is to analyze the biological response by detecting the degree of polarization or anisotropy of the fluorescence spectrum. The polarization angle associated with the fluorescence mechanism and electronic structure of organisms or materials has not been measured. In the polarization measurement, the default fluorescence polarization direction is consistent with the polarization direction of the incident light, that is, the polarization angle is 0 degrees. The actual situation is that the polarization angle changes with the wavelength of the incident light, and its value is uncert...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/21
Inventor 陈思颖牟涛涛张寅超郭磐陈和
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap