Method for continuously detecting glucose concentration in sample, reagent kit and method for using biosensor

Abstract

The invention relates to a method for continuously detecting the glucose concentration in a sample, a reagent kit and a method for using a biosensor. The method for continuously detecting the glucose concentration in the sample comprises the following steps of: (a) providing the biosensor, wherein the biosensor comprises a converter and polysaccharide covering on the surface of the converter; (b) providing a carbohydrate-binding protein solution with specific concentration, wherein carbohydrate-binding protein is at least provided with a receptor, and the receptor has the capability of being combined with carbohydrate and glucose; (c) mixing the sample to be detected and the carbohydrate-binding protein solution with the specific concentration into a mixture; (d) contacting the mixture with the biosensor; (e) detecting the quantity of the carbohydrate-binding protein combined with the carbohydrate through the biosensor, wherein the glucose concentration of the sample to be detected and the quantity of the carbohydrate-binding protein combined with the carbohydrate form an inverse relation; and (f) recovering the surface of the biosensor through a high-concentration glucose solution.

Description

technical field [0001] The invention relates to a glucose detection method, and in particular to a method for continuously detecting the glucose concentration of a sample to be tested. Background technique [0002] At present, most blood glucose detection is based on enzyme reaction, but the use of enzyme is not stable in continuous detection. In view of this, methods for detecting glucose using glucose acceptor proteins or other specific receptors (such as chelates) have also been developed successively to solve the problem that the enzyme activity is not easy to maintain. Among them, existing studies have demonstrated the feasibility of using a polysaccharide-lectin (polysaccharide-lectin) system to detect glucose. The current polysaccharide-lectin detection system uses the competition and aggregation characteristics of glucose, polysaccharides, and lectins as a detection method, especially in combination with fluorescence resonance energy transfer (FRET). ) effect detec...

AI Technical Summary

Problems solved by technology

However, some studies have pointed out that as the degree of agglutination and time increase, it will increase the difficulty of reversible reaction during detection and increase the time spent, which will affect the accuracy of detection. Therefore, the traditional polysaccharide-lectin system is not suitable for Continuous detection for a long time

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