2 results about "L glutamate" patented technology

This invention relates to microorganisms that produce L-glutamic acid and a method for producing amino acids. The method involves culturing microorganisms in a culture medium that have the ability to produce one or more amino acids selected from L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline, and L-arginine, and that have been modified to increase the activity of α-ketoglutarate synthase, thereby generating and accumulating the L-amino acids in the culture medium or in the cells, and collecting the L-amino acids from the culture medium or in the cells.

This invention discloses a culture medium and process for alleviating ammonium accumulation in cells. The concentrations of the substances added to the culture medium are as follows: 2 g / L glutamate, 0 g / L glutamine, 2 g / L aspartic acid, 2 g / L asparagine, 1 g / L serine, 1 g / L pyruvate, 0.5 g / L α-ketoglutarate, 0.5 g / L oxaloacetic acid, 0.5 g / L proline, 0 g / L glucose, 8 g / L galactose, and 0.1% glycerol. Compared with the prior art, the advantages of this application are: this method mainly achieves the purpose of maintaining cell viability and reducing the cell metabolic byproduct ammonium ions by adjusting the proportion of substances related to cell metabolic pathways and adding additional cell protectants; using the optimized culture medium of this invention, the cell viability can be increased from 42.38% to 95.21% and the ammonium ion concentration can be reduced from 6.308 mM to 0.914 mM by day 7.